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1.发明公开DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA's and their use in transforming microorganisms 失效编码各种成熟THAUMATIN的DNA序列，以及包含所述DNA的克隆载体及其在转化微生物中的用途
公开(公告)号：EP0054330A3
公开(公告)日：1982-09-22
申请号：EP81201354
申请日：1981-12-11
申请人： UNILEVER NV , UNILEVER PLC
发明人： Verrips, Cornelis Theodorus , Ledeboer, Adrianus Marinus , Edens, Luppo , Klok, Robert , Maat, Jan
摘要： The invention relates to DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA sequences and their use in transforming microorganisms.
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2.发明公开DNA molecules comprising the genes for preprochymosin and its maturation forms, and microorganisms transformed thereby 无效包含前体蛋白基因及其成熟形式的DNA分子和转化的微生物
公开(公告)号：EP0077109A3
公开(公告)日：1983-06-29
申请号：EP82201272
申请日：1982-10-13
申请人： UNILEVER PLC , UNILEVER NV
IPC分类号： C12N15/00 , C12N09/52 , C12N09/60 , C12N01/00 , C12R01/19 , C12R01/46 , C12R01/225 , C12R01/07 , C12R01/465
CPC分类号： C12N9/6481 , C12N9/52 , C12N9/60 , C12N15/102 , C12N15/71
摘要： The present invention relates to recombinant DNA and plasmids comprising specific structural genes of mammalian origin coding for the various allelic and maturation forms of preprochymosin, particularly those of bovine origin, and the use of said recombinant plasmids to transform microorganisms in which said genes are expressed.
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3.发明公开Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant DNA technology 失效在漂白和/或洗涤剂组合物中使用氧化剂及其由重组DNA技术工程的微生物制备
公开(公告)号：EP0173378A3
公开(公告)日：1986-08-06
申请号：EP85201235
申请日：1985-07-25
申请人： UNILEVER NV , UNILEVER PLC
发明人： Ledeboer, Adrianus Marinus , Maat, Jan , Verrips, Cornelis Theodorus , Visser, Christiaan , Janowicz, Zbigniew Alojzy , Hollenberg, Cornelis Petrus
IPC分类号： C12N15/00 , C12N01/16 , C11D03/386 , C11D03/395 , C12P21/02 , C07H21/04 , C12N09/02
CPC分类号： C12N9/0022 , A01K2217/05 , C07K14/60 , C07K2319/00 , C11D3/38654 , C12N9/0006 , C12N9/1022 , C12N15/68 , C12N15/81 , C12N15/815 , C12N15/90
摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.
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4.发明公开Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant DNA technology 失效在漂白和/或洗涤剂组合物中使用氧化剂及其由重组DNA技术工程制备的微生物
公开(公告)号：EP0423890A3
公开(公告)日：1991-07-03
申请号：EP90202731.7
申请日：1985-07-25
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： Ledeboer, Adrianus Marinus , Maat, Jan , Verrips, Cornelis Theodorus , Visser, Christiaan , Janowicz, Zbigniew Alojzy , Hollenberg, Cornelis Petrus
CPC分类号： C12N9/0022 , A01K2217/05 , C07K14/60 , C07K2319/00 , C11D3/38654 , C12N9/0006 , C12N9/1022 , C12N15/68 , C12N15/81 , C12N15/815 , C12N15/90
摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.
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5.发明公开Yeast of the genus Kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms 失效克鲁维酵母属，修改后用于Preprothaumatin的表达或它的各种等位基因和修饰形式或成熟形式的酵母。
公开(公告)号：EP0096910A1
公开(公告)日：1983-12-28
申请号：EP83200712.4
申请日：1983-05-19
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： Edens, Luppo , Ledeboer, Adrianus Marinus , Verrips, Cornelis Theodorus , Van Den Berg, Johannes Abel
CPC分类号： C12N9/2428 , C07K14/43 , C07K2319/036 , C12N9/6481 , C12N15/68 , C12N15/815 , C12N15/90
摘要： The invention relates to yeasts of the genus Kluyveromyces, in particular K. lactis, acting as hosts for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, which yeasts contain an rDNA plasmid comprising a structural gene encoding for a thaumatin-like protein, an autonomous replicating sequence derived from K. lactis (KARS), a yeast regulon derived from the GAPDH-genes of S. cerevisiae, and a selection marker, e.g. the trp-1 or the lac-4 gene from S. cerevisiae and K. lactis, respectively. Optionally the plasmid also contains a transcription terminator from a yeast. Further the preparation of such yeasts and the preparation of thaumatin-like proteins with such yeasts and the proteins so obtained are claimed. The yeast described give a better expression than obtained with E. coli as described in EP-PA (A2) 0054330 and 0054331, published on 23 June 1982.
摘要翻译： 本发明涉及一种克鲁维酵母属的酵母，特别是乳酸克鲁维酵母，充当用于preprothaumatin的表达或它的各种等位基因主机和修饰的形式或它们的成熟形式，其含有酵母rDNA的质粒，其包含的结构基因编码用于索马甜 样蛋白，从乳酸克鲁维酵母（KARS）衍生的自主复制序列，来自酿酒酵母的GAPDH基因的酵母调节子和选择标记，例如 在TRP-1或从分别酿酒酵母和乳酸克鲁维酵母，lac启动-4基因。 可选地，所以质粒含有酵母转录终止。 进一步寻求酵母菌的准备和索马甜样蛋白与酵母搜索编制和蛋白质这样获得的声称。 描述的酵母得到更好的表达比用大肠杆菌获得，如EP-PA（A2）中所描述0054330和0054331，6月23日公布的第一千九百八十二
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6.发明授权Structural genes encoding the various allelic and maturation forms of preprothaumatin and mutations thereof, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells 失效结构基因编码预处理药物及其突变体的各种异常和成熟形式，包含结构基因的重组克隆载体及其在转化的微生物宿主细胞中的表达
公开(公告)号：EP0054331B1
公开(公告)日：1992-06-03
申请号：EP81201355.5
申请日：1981-12-11
申请人： UNILEVER N.V. , UNILEVER PLC
CPC分类号： C07K14/43
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7.发明授权DNA molecules comprising the genes for preprochymosin and its maturation forms, and microorganisms transformed thereby 无效包含原核生物基因及其成熟形式的DNA分子和转化的微生物
公开(公告)号：EP0077109B1
公开(公告)日：1989-08-23
申请号：EP82201272.0
申请日：1982-10-13
申请人： UNILEVER N.V. , UNILEVER PLC
IPC分类号： C12N15/00 , C12N9/52 , C12N9/60 , C12N1/00 , C12R1/19 , C12R1/46 , C12R1/225 , C12R1/07 , C12R1/465
CPC分类号： C12N9/6481 , C12N9/52 , C12N9/60 , C12N15/102 , C12N15/71
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8.发明授权Yeast of the genus Kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms 失效基因改造的耶鲁大学学士学位论文修改为普伐他汀或其各种修饰形式或其成熟形式的表达
公开(公告)号：EP0096910B1
公开(公告)日：1989-04-19
申请号：EP83200712.4
申请日：1983-05-19
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： Edens, Luppo , Ledeboer, Adrianus Marinus , Verrips, Cornelis Theodorus , Van Den Berg, Johannes Abel
CPC分类号： C12N9/2428 , C07K14/43 , C07K2319/036 , C12N9/6481 , C12N15/68 , C12N15/815 , C12N15/90
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9.发明授权Process for preparing a polypeptide by culturing a transformed microorganism, a transformed microorganism suitable therefor and DNA sequences suitable for preparing such microorganism 失效通过培养转化的微生物制备多肽的方法，适用于其的转化微生物和适用于制备这种微生物的DNA序列
公开(公告)号：EP0173378B1
公开(公告)日：1991-06-12
申请号：EP85201235.0
申请日：1985-07-25
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： Ledeboer, Adrianus Marinus , Maat, Jan , Verrips, Cornelis Theodorus , Visser, Christiaan , Janowicz, Zbigniew Alojzy , Hollenberg, Cornelis Petrus
CPC分类号： C12N9/0022 , A01K2217/05 , C07K14/60 , C07K2319/00 , C11D3/38654 , C12N9/0006 , C12N9/1022 , C12N15/68 , C12N15/81 , C12N15/815 , C12N15/90
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10.发明公开Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant DNA technology 失效在漂白使用氧化还原酶和清洗剂和通过重组DNA转化的微生物制备它们。
公开(公告)号：EP0423890A2
公开(公告)日：1991-04-24
申请号：EP90202731.7
申请日：1985-07-25
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： Ledeboer, Adrianus Marinus , Maat, Jan , Verrips, Cornelis Theodorus , Visser, Christiaan , Janowicz, Zbigniew Alojzy , Hollenberg, Cornelis Petrus
CPC分类号： C12N9/0022 , A01K2217/05 , C07K14/60 , C07K2319/00 , C11D3/38654 , C12N9/0006 , C12N9/1022 , C12N15/68 , C12N15/81 , C12N15/815 , C12N15/90
摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts.
Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale.
Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.摘要翻译： 结构基因和醇氧化酶（MOX）和多形汉逊的二羟基丙酮合酶（DHAS）的它们的调节DNA序列已经被分离和核苷酸序列确定性开采。 本发明涉及使用了MOX基因，以及组合使用MOX和/或DAS的调节DNA序列的与MOX基因，修饰后OPTIONALLY物，或其他氧化酶的基因，或其他的基因，以产生 改造的微生物，特别是酵母。 所述工程微生物可以产生在产量氧化酶或其它酶确实允许大规模工业应用。 更完了，所述改造的微生物可产生具有相对于它们在氧化反应中的应用和/或在漂白和洗涤剂产品改进的特性的氧化酶。
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