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    Improvements in the expression of newly introduced genes in yeast cells
    1.
    发明公开
    Improvements in the expression of newly introduced genes in yeast cells 失效
    在YEAST细胞中新引入基因表达的改进

    公开(公告)号：EP0129268A3

    公开(公告)日：1985-06-19

    申请号：EP84200735

    申请日：1984-05-21

    申请人： UNILEVER NV ,  UNILEVER PLC

    发明人： Edens, Luppo ,  Russell, Stephen William ,  Visser, Christiaan ,  Verrips, Cornelis Theodorus

    IPC分类号： C12N15/00 ,  C12P21/02 ,  C07H21/04 ,  C12N01/16 ,  C12R01/645

    CPC分类号： C12N9/6481 ,  C07K14/43 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/625 ,  C12N15/81 ,  C12N15/815

    摘要： The invention relates to an improved expression system for newly introduced genes in yeast and comprises a yeast regulon, and preferably a transcription terminator, derived from one of the GAPDH genes of S. cerevisiae. The about 850 nucleotides long GAPDH regulon described proved to be almost ten times as effective as smaller regulons described previously. Said regulon and/orterminator can be introduced into yeasts either as part of plasmid molecules or by incorporating into the yeast genome. Vectors containing the expression system preferably comprise an autonomously replicating sequence derived from K. lactis (KARS) or an origin of replication originating from the S. cerevisiae 2 micron yeast plasmid. After transformation of yeasts, in particular of the genera Kluyveromyces and Saccharomyces, with said vectors, the yeasts transformed can produce foreign proteins more effectively. In the case ofthaumatin the presence of signal polypeptide (the pre-part) appears to be essential for expression in yeast. DNA sequence encoding other signal polypeptides are also described. The use of codons preferred by yeast in both structural genes, preferably those encoding thaumatin- like proteins and chymosin-like proteins, and DNA sequences encoding signal polypeptides is also described.

    Process for the preparation of an oil-in-water emulsion
    2.
    发明公开
    Process for the preparation of an oil-in-water emulsion 失效
    制备水包油乳液的方法

    公开(公告)号：EP0028428A3

    公开(公告)日：1981-05-27

    申请号：EP80200978

    申请日：1980-10-16

    申请人： UNILEVER NV ,  UNILEVER PLC

    发明人： Verrips, Cornelis Theodorus ,  Van Rhee, Renée

    IPC分类号： A23L01/34 ,  A23L01/24

    CPC分类号： A23L27/60

    摘要： Oil-in-water emulsions, which are microbiologically stable upon storage, consisting of oil, water, thickeners, emulsifiers, and optional further ingredients, are obtained by subjecting at least one of these components to an osmotic and/or acid shock and to a temperature shock by heating said component(s) within 3 minutes, preferably within 1 minute, to 40°-55°C, preferably to 45°-50°C, and processing the thus treated component(s) with the remaining ingredients into a final emulsion, during which the temperature of the final emulsion up to and including the filling and hermetically sealing of the containers into which it is being packed, is maintained at 40°-55°C. Preferably the temperature shock is achieved by adding during the preparation of the final emut- sion a thickener suspension having a temperature of 70°-95°C to the remaining components which have been subjected to an osmotic and/or acid shock.