公开(公告)号：EP1606415A4

公开(公告)日：2006-08-02

申请号：EP03729793

申请日：2003-05-06

申请人： UNIV TSINGHUA ,  CAPITAL BIOCHIP CO LTD

发明人： TAO SHENGE ,  CHENG JING ,  MAX XUMEI ,  ZHOU YUXIANG ,  ZHANG QIONG

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14

CPC分类号： B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

摘要： This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

公开(公告)号：EP1639127A4

公开(公告)日：2007-04-11

申请号：EP03817285

申请日：2003-07-18

申请人： UNIV TSINGHUA ,  CAPITALBIO CORP

发明人： GAO HUAFANG ,  MA XUEMEI ,  ZHANG CHI ,  CHEN QIAN ,  ZHOU YIMING ,  WANG DONG ,  ZHANG YIZHE ,  TIAN YUE ,  ZHANG RUI ,  LAN GENGXIN ,  ZHOU YUXIANG ,  CHENG JING

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837

公开(公告)号：EP1951894A4

公开(公告)日：2009-04-08

申请号：EP06705658

申请日：2006-02-20

申请人： CAPITALBIO CORP ,  UNIV TSINGHUA

发明人： GAO HUAFANG ,  LI ZE ,  WANG DONG ,  LIU YANHUA ,  LIU XIANG ,  JIANG YANGZHOU ,  ZHAO CHUANZAN ,  LI LI ,  LAN GENGXIN ,  GUO TAO ,  CAI BIN ,  XING WANLI ,  ZHOU YUXIANG ,  CHENG JING

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6837 ,  C12Q2600/156

摘要： The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.

公开(公告)号：EP1654381A4

公开(公告)日：2006-10-18

申请号：EP03818072

申请日：2003-08-27

申请人： UNIV TSINGHUA ,  CAPITALBIO CORP

发明人： WANG DONG ,  LI GANG ,  MA XUEMEI ,  LIU CHENGXUN ,  ZHOU YUXIANG ,  CHENG JING

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6806 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2565/513 ,  C12Q2565/507

摘要： This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification.

公开(公告)号：EP1816476A4

公开(公告)日：2008-08-20

申请号：EP04797371

申请日：2004-11-23

申请人： CAPITALBIO CORP ,  UNIV TSINGHUA

发明人： SHAO WEI ,  ZHAO YONGCHAO ,  SUN YIMIN ,  QIAO JIYING ,  WEI HUAJIANG ,  YANG WEIPING ,  ZHOU YUXIANG ,  CHENG JING

IPC分类号： G01N33/68 ,  C12Q1/68 ,  G01N33/543

CPC分类号： G01N33/54366 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2565/531 ,  C12Q2565/501 ,  C12Q2563/131

摘要： A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.

公开(公告)号：EP1495088A4

公开(公告)日：2007-07-04

申请号：EP03720096

申请日：2003-04-15

申请人： UNIV TSINGHUA ,  CAPITALBIO CORP

发明人： YI GUANGSHUN ,  SUN BAOQUAN ,  CHEN DEPU ,  ZHOU YUXIANG ,  CHENG JING ,  YANG WENJUN ,  GE YUE ,  GUO LIANGHONG

IPC分类号： C09K11/85 ,  C09K11/08 ,  C09K11/77 ,  C09K11/61

CPC分类号： C09K11/7772 ,  C09K11/7773 ,  Y10S977/83 ,  Y10S977/834

摘要： Nanometer-scaled up-converting fluoride phosphor particles and processes of making them are disclosed. In the process, an aqueous solution consisting of soluble salts of rare-earth metal ions at a molar ratio of (yttrium, lanthanum or gadolinium): ytterbium:(erbium, holmium, terbium or thulium) = (70-90):(0-29):(0.001-15) is mixed a rare-earth metal chelator and a soluble fluoride salt to form precipitates, which are then annealed at an elevated temperature to produce nanometer-scaled up-converting fluoride phosphor particles. The particle size is between 35nm and 200nm, and can be controlled by the amount of the metal chelator added to the solution. The nanometer-sized particle is applicable to many biological assays.