-
公开(公告)号：EP1809664A1
公开(公告)日：2007-07-25
申请号：EP04821383.9
申请日：2004-11-09
申请人： USV Limited
发明人： BORBHUIYA, Ahmed Monsur , RAO, Yada Madhava , RAO, Laxmi Srinivas , MAITI, Dipanwita , NIPHADKAR, Prabhakar Milind , MISHRA, Shrikant
摘要： The present invention relates to a commercial process for purification of human Growth hormone obtained by DNA recombinant technique using hydrophobic interactive chromatography, efficient to separate clipped molecules of human growth hormone present as an impurity arising due to clipping positions present on the megamolecule of human growth hormone from the target human growth hormone molecule using a phenyl column, mixture of aqueous buffers and organic solvents as an eluant, followed by desalting, lypholization and the invention also relates to the process wherein the elution of clipped molecules of human growth hormone precedes the elution of target human growth hormone to obtain hGH of purity > 99.5%.
-
公开(公告)号：EP2356240A2
公开(公告)日：2011-08-17
申请号：EP09810850.9
申请日：2009-10-28
申请人： USV Limited
发明人： RAO, Laxmi Srinivas , MISHRA, Shrikant , NIPHADKAR, Milind , THAKUR, Priti , NAZARETH, Genevieve
CPC分类号： C12N15/70
摘要： An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E. coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between −55 to −10 and ribosome binding site from the transcription initiation site.
-
公开(公告)号：EP1809664B1
公开(公告)日：2008-05-14
申请号：EP04821383.9
申请日：2004-11-09
申请人： USV Limited
发明人： BORBHUIYA, Ahmed Monsur , RAO, Yada Madhava , RAO, Laxmi Srinivas , MAITI, Dipanwita , NIPHADKAR, Prabhakar Milind , MISHRA, Shrikant
摘要： The present invention relates to a commercial process for purification of human Growth hormone obtained by DNA recombinant technique using hydrophobic interactive chromatography, efficient to separate clipped molecules of human growth hormone present as an impurity arising due to clipping positions present on the megamolecule of human growth hormone from the target human growth hormone molecule using a phenyl column, mixture of aqueous buffers and organic solvents as an eluant, followed by desalting, lypholization and the invention also relates to the process wherein the elution of clipped molecules of human growth hormone precedes the elution of target human growth hormone to obtain hGH of purity > 99.5%.
-
-
搜索结果
3 条记录 (耗时 0.008 秒)
