公开(公告)号：EP2173768A1

公开(公告)日：2010-04-14

申请号：EP07870535.7

申请日：2007-12-06

申请人： USV Limited

发明人： SANKARARAMAN, Uma ,  MAITI, Dipanwita ,  SANKARANKUTTY, Meera ,  SHEKHAWAT, Rakesh ,  KOPPARAPU, Narasimha, Kumar ,  ZAIDI, Gulnaz ,  RATHOD, Bipinchandra ,  THAKAR, Niren, Praful ,  THAKUR, Priti ,  CHUTKE, Anjali ,  MISHRA, Shrikant

IPC分类号： C07K14/635 ,  C07K1/36

CPC分类号： C07K14/635 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/50 ,  C07K2319/61

摘要： The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragemt as set forth in SEQ. ID. No.:l encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partrner consisting of 41 amino acids belonging to Escherichia coli β-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transforming Escherichia coli with the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in Escherichia coli. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP- HPLC to yield a target protein of ≥ 99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).

公开(公告)号：EP2356240A2

公开(公告)日：2011-08-17

申请号：EP09810850.9

申请日：2009-10-28

申请人： USV Limited

发明人： RAO, Laxmi Srinivas ,  MISHRA, Shrikant ,  NIPHADKAR, Milind ,  THAKUR, Priti ,  NAZARETH, Genevieve

IPC分类号： C12N15/70 ,  C12R1/19

CPC分类号： C12N15/70

摘要： An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E. coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between −55 to −10 and ribosome binding site from the transcription initiation site.

公开(公告)号：EP2173768B1

公开(公告)日：2014-03-05

申请号：EP07870535.7

申请日：2007-12-06

申请人： USV Limited

发明人： SANKARARAMAN, Uma ,  MAITI, Dipanwita ,  SANKARANKUTTY, Meera ,  SHEKHAWAT, Rakesh ,  KOPPARAPU, Narasimha Kumar ,  ZAIDI, Gulnaz ,  RATHOD, Bipinchandra ,  THAKAR, Niren Praful ,  THAKUR, Priti ,  CHUTKE, Anjali ,  MISHRA, Shrikant

IPC分类号： C07K14/635 ,  C07K1/36

CPC分类号： C07K14/635 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/50 ,  C07K2319/61

公开(公告)号：EP1981978B1

公开(公告)日：2011-01-19

申请号：EP07736519.5

申请日：2007-02-07

申请人： USV Limited

发明人： RAO, Laxmi, S. ,  MISHRA, Shrikant ,  BORBHUIYA, Monsur, Ahmed ,  MHATRE, Deepa ,  THAKUR, Priti

IPC分类号： C12N15/62

CPC分类号： C12N15/62

摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.

公开(公告)号：EP1981978A2

公开(公告)日：2008-10-22

申请号：EP07736519.5

申请日：2007-02-07

申请人： USV Limited

发明人： RAO, Laxmi, S. ,  MISHRA, Shrikant ,  BORBHUIYA, Monsur, Ahmed ,  MHATRE, Deepa ,  THAKUR, Priti

IPC分类号： C12N15/62

CPC分类号： C12N15/62

摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.

摘要翻译： 本公开提供了用于纯化重组生物活性蛋白或多肽的亲和多肽。 此外，本公开内容提供了融合重组蛋白或多肽，其中融合重组蛋白包含至少两种组分，感兴趣的生物活性多肽或蛋白质或蛋白质和亲和性多肽。 生物活性多肽可通过共价结合直接或间接连接至亲和性多肽。 本公开提供了用于在宿主细胞中产生所述融合重组蛋白的重组表达载体。 此外，本公开内容提供了从宿主细胞纯化重组蛋白的改进方法。 此外，本公开提供了通过固定金属离子螯合色谱法纯化重组生物活性多肽或蛋白质的方法。