公开(公告)号：EP1356070A2

公开(公告)日：2003-10-29

申请号：EP01973503.4

申请日：2001-09-21

申请人： Virxsys

发明人： CHANG, Yung-Nien ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  CONDE, Betty ,  DAVIS, Brian ,  YU, Qiao ,  YANG, Yanping ,  MERLING, Randal ,  HAN, Wei ,  NI, Yajin ,  LI, Yuexia ,  DROPULIC, Boro

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043 ,  C12N2800/108 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention provides improved conditionally replicating vectors that have improved safety against the generation of replication competent vectors or virus. Also disclosed are methods of making, propagating and selectively packaging, modifying, and using such vectors. Included are improved helper constructs, host cells, for use with the improved vectors as well as pharmaceutical compositions and host cells comprising the vectors, the use of vector containing host cells to screen drugs, and methods of using the vectors to determine gene function. The methods also include the prophylatic and therapeutic treatment of disease, especially viral infection, and HIV infection in particular.

公开(公告)号：EP1315823A2

公开(公告)日：2003-06-04

申请号：EP01966434.1

申请日：2001-08-29

申请人： VIRxSYS Corporation

发明人： HUMEAU, Laurent ,  HAN, Wei ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  LESHER, Mechelle ,  DAVIS, Brian ,  CHANG, Yung-Nien ,  DROPULIC, Boro

IPC分类号： C12N15/87 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043

摘要： The present invention provides methods, as well as compositions related thereto, for the efficient transduction of cells using viral vectors. The efficiency of transduction is increased by contacting the cell to be transduced with one or more molecules that bind the cell surface. Contact with a cell surface binding molecule may occur before, after, or simultaneously with contact between the viral vector and the cell. The transduced vectors may be constructed to express a gene of interest, permitting the transduced cells to be used as therapeutic and prophylactic agents.