公开(公告)号：EP1356070A2

公开(公告)日：2003-10-29

申请号：EP01973503.4

申请日：2001-09-21

申请人： Virxsys

发明人： CHANG, Yung-Nien ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  CONDE, Betty ,  DAVIS, Brian ,  YU, Qiao ,  YANG, Yanping ,  MERLING, Randal ,  HAN, Wei ,  NI, Yajin ,  LI, Yuexia ,  DROPULIC, Boro

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043 ,  C12N2800/108 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention provides improved conditionally replicating vectors that have improved safety against the generation of replication competent vectors or virus. Also disclosed are methods of making, propagating and selectively packaging, modifying, and using such vectors. Included are improved helper constructs, host cells, for use with the improved vectors as well as pharmaceutical compositions and host cells comprising the vectors, the use of vector containing host cells to screen drugs, and methods of using the vectors to determine gene function. The methods also include the prophylatic and therapeutic treatment of disease, especially viral infection, and HIV infection in particular.

公开(公告)号：EP1677832A1

公开(公告)日：2006-07-12

申请号：EP04788661.9

申请日：2004-09-09

申请人： VIRxSYS Corporation

发明人： LU, Xiaobin ,  DROPULIC, Boro

IPC分类号： A61K48/00 ,  A61K39/21

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/86 ,  C12N2510/02 ,  C12N2740/16034 ,  C12N2740/16043 ,  C12N2760/20234

摘要： The present invention relates to multiple novel approaches for the generation of an immune response in humans using lentivirus-based vector technology. The invention provides for the ability to mimic the efficacy of a live attenuated (LA) vaccine, without exposing the patient to the risk of disease as possible with some LA vaccines. The invention thus provides for the systems of complementary conditionally replicating vectors, vectors that produce replication deficient virus like particles, and multi-antigen constructs that target a virus or microbial pathogen. The use of these materials in the practice of the invention permits the generation of robust cellular and humoral responses to the antigens presented thereby.

公开(公告)号：EP1315823A2

公开(公告)日：2003-06-04

申请号：EP01966434.1

申请日：2001-08-29

申请人： VIRxSYS Corporation

发明人： HUMEAU, Laurent ,  HAN, Wei ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  LESHER, Mechelle ,  DAVIS, Brian ,  CHANG, Yung-Nien ,  DROPULIC, Boro

IPC分类号： C12N15/87 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043

摘要： The present invention provides methods, as well as compositions related thereto, for the efficient transduction of cells using viral vectors. The efficiency of transduction is increased by contacting the cell to be transduced with one or more molecules that bind the cell surface. Contact with a cell surface binding molecule may occur before, after, or simultaneously with contact between the viral vector and the cell. The transduced vectors may be constructed to express a gene of interest, permitting the transduced cells to be used as therapeutic and prophylactic agents.

公开(公告)号：EP1746877A2

公开(公告)日：2007-01-31

申请号：EP05750613.1

申请日：2005-05-17

申请人： VIRxSYS Corporation

发明人： LU, Xiaobin ,  DROPULIC, Boro ,  SLEPUSHKIN, Vladimir ,  BINDER, Gwendolyn

IPC分类号： A01K67/027

CPC分类号： C12N15/86 ,  C12N15/111 ,  C12N2310/121 ,  C12N2310/127 ,  C12N2740/16052

摘要： The present invention provides a recombinant transcription unit capable of producing an RNA transcript of a predetermined size comprising a regulatory sequence operably linked to a nucleotide sequence comprising a transcribed region such that the transcription of said transcribed region is controlled by said regulatory sequence. The transcribed region comprises a region that encodes for a viral sequence, and a non-coding region downstream of the region encoding for said viral sequence, wherein the non-coding region comprises a nucleotide sequence encoding a cis-acting ribozyme. Methods of using the recombinant transcription unit, and cells containing vectors comprising the recombinant transcription unit are also disclosed.