公开(公告)号：EP1356070A2

公开(公告)日：2003-10-29

申请号：EP01973503.4

申请日：2001-09-21

申请人： Virxsys

发明人： CHANG, Yung-Nien ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  CONDE, Betty ,  DAVIS, Brian ,  YU, Qiao ,  YANG, Yanping ,  MERLING, Randal ,  HAN, Wei ,  NI, Yajin ,  LI, Yuexia ,  DROPULIC, Boro

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043 ,  C12N2800/108 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention provides improved conditionally replicating vectors that have improved safety against the generation of replication competent vectors or virus. Also disclosed are methods of making, propagating and selectively packaging, modifying, and using such vectors. Included are improved helper constructs, host cells, for use with the improved vectors as well as pharmaceutical compositions and host cells comprising the vectors, the use of vector containing host cells to screen drugs, and methods of using the vectors to determine gene function. The methods also include the prophylatic and therapeutic treatment of disease, especially viral infection, and HIV infection in particular.

公开(公告)号：EP1769078A1

公开(公告)日：2007-04-04

申请号：EP05768137.1

申请日：2005-06-30

申请人： VIRxSYS Corporation

发明人： HUMEAU, Laurent ,  SLEPUSHKIN, Vladimir ,  PASZKIET, Brian ,  NI, Yajin

IPC分类号： C12N15/867 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2740/15052 ,  C12N2810/6081 ,  C12N2810/80 ,  C12N2810/855

摘要： The invention relates to, a method of increasing vector transduction in target cells. The invention provides for the recombinant engineering of a packaging cell line to be capable of expressing one or more membrane proteins which facilitate binding to, and activation of, a target cell. The invention also provides for recombinant engineering of a cell that endogenously expresses one or more such membrane proteins into a packaging cell line. A vector packaged into viral particles via use of such cell lines would comprise an outer envelope containing these proteins. The particles would be specifically suited for binding and targeting to a target cell to facilitate transduction thereof with the vector. The target cell may also be simultaneously activated (stimulated) by the packaged vector in the absence of exogenously supplied stimulatory molecules.

摘要翻译： 本发明涉及增加靶细胞中载体转导的方法。 本发明提供了包装细胞系的重组工程改造，使其能够表达一种或多种促进结合和激活靶细胞的膜蛋白。 本发明还提供了将一种或多种这种膜蛋白内源地表达到包装细胞系中的细胞的重组工程。 通过使用这种细胞系包装成病毒颗粒的载体将包含含有这些蛋白质的外包膜。 该颗粒特别适合于结合并靶向靶细胞以促进其与载体的转导。 靶细胞也可以在没有外源提供的刺激分子的情况下由包装的载体同时激活（刺激）。

公开(公告)号：EP1315823A2

公开(公告)日：2003-06-04

申请号：EP01966434.1

申请日：2001-08-29

申请人： VIRxSYS Corporation

发明人： HUMEAU, Laurent ,  HAN, Wei ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  LESHER, Mechelle ,  DAVIS, Brian ,  CHANG, Yung-Nien ,  DROPULIC, Boro

IPC分类号： C12N15/87 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043

摘要： The present invention provides methods, as well as compositions related thereto, for the efficient transduction of cells using viral vectors. The efficiency of transduction is increased by contacting the cell to be transduced with one or more molecules that bind the cell surface. Contact with a cell surface binding molecule may occur before, after, or simultaneously with contact between the viral vector and the cell. The transduced vectors may be constructed to express a gene of interest, permitting the transduced cells to be used as therapeutic and prophylactic agents.

公开(公告)号：EP2271754A1

公开(公告)日：2011-01-12

申请号：EP09724469.3

申请日：2009-03-27

申请人： VIRxSYS Corporation

发明人： LEMIALE, Franck, Yann ,  KOROKHOV, Nikolay ,  HUMEAU, Laurent, M. ,  SLEPUSHKIN, Vladimir

IPC分类号： C12N15/00

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10043 ,  C12N2740/16234 ,  C12N2740/16322 ,  C12N2740/16334 ,  C12N2760/20234 ,  C12N2830/48 ,  C12N2830/60

摘要： The present invention provides for lentiviral vectors for vaccine delivery comprising a 5' long terminal repeat (LTR) and a 3' LTR, an integrated nucleic acid sequence, wherein the integrated nucleic acid sequence is expressed by the 5' LTR; and a nucleic acid sequence encoding functional REV coding sequence and a rev response element (RRE)-containing sequence, wherein the RRE-containing sequence is located upstream of the REV coding sequence, and wherein transcription of said first nucleic acid sequence and said second nucleic acid sequence is driven by said 5' LTR. Also provided for are pharmaceutical compositions, methods of making and using the lentiviral vectors of the present invention.

公开(公告)号：EP1888758A2

公开(公告)日：2008-02-20

申请号：EP06770822.2

申请日：2006-05-22

申请人： VIRxSYS Corporation

发明人： SLEPUSHKIN, Vladimir ,  HUMEAU, Laurent

IPC分类号： C12N15/867 ,  A61K48/00

CPC分类号： A01K67/0271 ,  A61K48/00 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2501/26 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2799/027

摘要： Methods, compositions, and systems relating to the stable transduction of primary cells of the hematopoietic system and/or hematopoietic stem cells. A method for stable transduction of primary cells of the hematopoietic system and/or hematopoietic stem cells, comprising contacting, in vitro or ex vivo, the surface of the cells with both a lentiviral vector and at least one molecule which binds the cell surface, and culturing the cells in a ventilated vessel comprising two or more layers under conditions conducive to growth and/or proliferation, wherein the vessel is suitable for culturing at least about 100 million cells. Use of the transduced primary cells to treat, diagnose, alleviate or prevent a tumor or infection in a subject is also disclosed. A system comprising a vessel or flask to grow the primary cells in is also described.

公开(公告)号：EP1746877A2

公开(公告)日：2007-01-31

申请号：EP05750613.1

申请日：2005-05-17

申请人： VIRxSYS Corporation

发明人： LU, Xiaobin ,  DROPULIC, Boro ,  SLEPUSHKIN, Vladimir ,  BINDER, Gwendolyn

IPC分类号： A01K67/027

CPC分类号： C12N15/86 ,  C12N15/111 ,  C12N2310/121 ,  C12N2310/127 ,  C12N2740/16052

摘要： The present invention provides a recombinant transcription unit capable of producing an RNA transcript of a predetermined size comprising a regulatory sequence operably linked to a nucleotide sequence comprising a transcribed region such that the transcription of said transcribed region is controlled by said regulatory sequence. The transcribed region comprises a region that encodes for a viral sequence, and a non-coding region downstream of the region encoding for said viral sequence, wherein the non-coding region comprises a nucleotide sequence encoding a cis-acting ribozyme. Methods of using the recombinant transcription unit, and cells containing vectors comprising the recombinant transcription unit are also disclosed.