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公开(公告)号：EP1309726B2
公开(公告)日：2018-10-03
申请号：EP01922870.9
申请日：2001-03-30
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , University of Massachusetts
IPC分类号： C12N15/11
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
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公开(公告)号：EP1309726B1
公开(公告)日：2009-12-02
申请号：EP01922870.9
申请日：2001-03-30
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , University of Massachusetts
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
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公开(公告)号：EP1309726A2
公开(公告)日：2003-05-14
申请号：EP01922870.9
申请日：2001-03-30
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , Max-Planck-Gesellschaft zur Förderungder Wissenschaften e.V. , Massachusetts Institute of Technology , UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
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公开(公告)号：EP3199631B1
公开(公告)日：2019-01-30
申请号：EP17160119.8
申请日：2001-11-29
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公开(公告)号：EP2386637B1
公开(公告)日：2018-05-16
申请号：EP11175587.2
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP2428569B1
公开(公告)日：2018-05-23
申请号：EP11175595.5
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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7.发明公开INDUCIBLE SMALL INTERFERING RNA (SIRNA) EXPRESSION CONSTRUCTS FOR TARGETED GENE SILENCING 有权基因诱导siRNA表达构建了靶向基因沉默
公开(公告)号：EP1583832A2
公开(公告)日：2005-10-12
申请号：EP04702315.5
申请日：2004-01-15
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , A01K2217/05 , A01K2217/075 , A61K38/00 , A61K48/0058 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
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公开(公告)号：EP2428570B1
公开(公告)日：2018-05-23
申请号：EP11175598.9
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1430128B1
公开(公告)日：2018-04-25
申请号：EP02782801.1
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： C12N15/113 , A61K38/00 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1407044B1
公开(公告)日：2007-09-19
申请号：EP01985833.1
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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