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1.发明公开SPINOSAD HETEROLOGOUS EXPRESSION STRAIN AND CONSTRUCTION METHOD THEREOF AND USE 审中-公开SPINOSAD异源表达菌株及其构建方法和应用
公开(公告)号：EP3219788A1
公开(公告)日：2017-09-20
申请号：EP14905979.2
申请日：2014-11-14
发明人： HUANG, Jun , YU, Zhen , LI, Meihong , ZHENG, Linghui , LI, Na , WANG, Haibin , BAI, Hua
摘要： The invention provides a construction method of spinosad heterologous expression strain, a spinosad heterologous expression strain obtained by the method and use thereof in preparing spinosad. The method utilizes a plurality of homologous recombination to replace the erythromycin synthetic gene cluster of Saccharopolyspora erythraea with the spinosad synthetic gene cluster and the rhamnose synthetic gene cluster, such that the Saccharopolyspora erythraea produces spinosad.
摘要翻译： 本发明提供了多杀菌素异源表达菌株的构建方法，由该方法获得的多杀菌素异源表达菌株及其在制备多杀菌素中的应用。 该方法利用多杀菌素同源重组，用多杀菌素合成基因簇和鼠李糖合成基因簇代替红色糖多孢菌红霉素合成基因簇，以使糖多孢菌红霉菌产生多杀菌素。
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2.发明公开RECOMBINANT MICROORGANISM EXPRESSING AVERMECTIN ANALOGUE AND USE THEREOF 有权AVEMECTIN-ANALOGON EXPRIMIERENDER REKOMBINANTER MIKROORGANISMUS UND DAVON VERWENDUNG
公开(公告)号：EP3118305A1
公开(公告)日：2017-01-18
申请号：EP14885605.7
申请日：2014-04-28
发明人： HUANG, Jun , WANG, Jidong , CHEN, Anliang , DENG, Aiwen , LIN, Jiatan , YU, Zhen , LI, Meihong , LI, Na , WANG, Haibin , ZHENG, Linghui , BAI, Hua
CPC分类号： A01N43/90 , C12N15/76 , C12P19/623 , C12R1/465
摘要： The present invention relates to a recombinant microorganism expressing avermectin or analogues thereof and construction method thereof, and also relates to a method of producing avermectin or analogues thereof using the recombinant microorganism, and avermectin or analogues thereof obtained using the method. In addition, the present invention further relates to uses of the avermectin or analogues thereof as insecticides. Using the recombinant microorganism of the present invention to produce avermectin or analogues thereof has numerous advantages, for example comprising at least one of the following: good stability, high yield, simple process, environmentally friendly, and greatly saving of production costs.
摘要翻译： 本发明涉及表达除虫菌素或其类似物的重组微生物及其构建方法，还涉及使用该方法得到的重组微生物和除虫菌素或其类似物来生产除虫菌素或其类似物的方法。 此外，本发明还涉及除虫菌素或其类似物作为杀虫剂的用途。 使用本发明的重组微生物来生产除虫菌素或其类似物具有许多优点，例如包括以下至少一种：良好的稳定性，高产率，简单的工艺，环保且大大节省生产成本。
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公开(公告)号：EP3118305B1
公开(公告)日：2019-08-14
申请号：EP14885605.7
申请日：2014-04-28
发明人： HUANG, Jun , WANG, Jidong , CHEN, Anliang , DENG, Aiwen , LIN, Jiatan , YU, Zhen , LI, Meihong , LI, Na , WANG, Haibin , ZHENG, Linghui , BAI, Hua
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