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    SYSTEMS AND METHODS FOR CONTINUOUS FLOW PCR SYSTEMS
    3.
    发明公开
    SYSTEMS AND METHODS FOR CONTINUOUS FLOW PCR SYSTEMS 审中-公开
    系统和方法PCR系统与直通

    公开(公告)号：EP2694982A1

    公开(公告)日：2014-02-12

    申请号：EP12714909.4

    申请日：2012-04-06

    申请人： Stokes Bio Limited

    发明人： AGUANNO, Mauro ,  GAUGHRAN, Mark ,  KINAHAN, David ,  KORENKE, Mark ,  LOUGH, Matthew ,  TALBOT, Ryan

    IPC分类号： G01N35/08

    CPC分类号： C12Q3/00 ,  B01L7/5255 ,  B01L2300/0627 ,  C12Q1/686 ,  G01N35/08 ,  G01N2035/00366

    摘要： A liquid handling system of a PCR system is instructed to obtain a matrix of samples and reagents for a PCR experiment. A fluid pumping system of the PCR system is instructed to maintain a continuous flow of a transport fluid through a plurality of micro-channels that allows the mixture of the samples and the reagents producing a plurality of mixed sample droplets. One or more post-bridge detection values are received from a post-bridge detection system of the PCR system for each mixed sample droplet to determine if the mixed sample droplet is mixed correctly. A thermocycler of the PCR system is instructed to maintain one or more temperatures for cycling the temperature of the plurality of mixed sample droplets. One or more endpoint detection values are received from an endpoint detection system of the PCR system for each mixed sample droplet to analyze the PCR experiment.

    SAMPLING DEVICE
    10.
    发明公开
    SAMPLING DEVICE 审中-公开
    采样装置

    公开(公告)号：EP2553420A2

    公开(公告)日：2013-02-06

    申请号：EP11760345.6

    申请日：2011-03-25

    申请人： Stokes Bio Limited

    发明人： DAVIES, Mark ,  DALTON, Tara ,  SAYERS, Michael ,  CHAWKE, Brian

    IPC分类号： G01N1/10 ,  G01N35/10 ,  B01L3/00

    CPC分类号： B01L3/021 ,  B01L3/0293 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  Y10T436/25 ,  Y10T436/25625

    摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfludic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.

    摘要翻译： 本发明一般涉及用于在不将气体引入微流体系统（例如液体桥系统）中的情况下获取和/或分配样品的装置，系统和方法。 示例性实施例提供了一种采样装置，其包括外护套; 所述护套内的多个管，其中所述管中的至少一个获取样本，并且至少一个管排出与所述样本不混溶的流体，其中获取所述样本的所述至少一个管可延伸超过 护套的远端并且可缩回到护套内; 以及连接到护套远端部分的阀，当管延伸超过远端时阀门打开，并且当管缩回到护套内时关闭。