公开(公告)号：EP2407545A4

公开(公告)日：2013-06-19

申请号：EP10750256

申请日：2010-03-10

申请人： FUNDACAO OSWALDO CRUZ

发明人： TENDLER MIRIAM ,  VILAR MONICA MAGNO ,  ARMOA GERALDO RODRIGUES GARCIA ,  MCINTOSH DOUGLAS ,  SIMPSON ANDREW J G

IPC分类号： C12N15/76 ,  A61P31/06 ,  A61P33/10 ,  A61P35/00

CPC分类号： A61K35/74 ,  A61K39/0003 ,  A61K39/002 ,  A61K39/04 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/43559

摘要： The present invention relates to a vaccinal strain of recombinant BCG Mycobacterium bovis expressing the Schistossoma mansoni Sm14 protein (BCG Pasteur ”leuD/p”K410-hsp60*-Sm14). The vaccinal strain of the present invention is employed in the control of infections caused by parasites, specially the Schistossoma mansoni. The vaccinal strain is a an auxotrophic strain to leucine amino acid derived from the BCG Pasteur sub-strain complemented to leucine after genetic transformation with the construct p”K410-hsp60*-Sm14. The efficacy of the BCG Pasteur ”leuD/p”K410-hsp60*-Sm14 strain in the control of the Schistossoma mansoni infection based on the recombinant Sm14 antigen in vivo is showed in the present invention.

公开(公告)号：EP1363937B1

公开(公告)日：2008-11-05

申请号：EP02727341.6

申请日：2002-03-01

申请人： Forschungszentrum Borstel Zentrum für Medizin und Biowissenschaften

发明人： SCHRAMM, Gabriele ,  HAAS, Helmut ,  FALCONE, Franco ,  GRONOW, Achim ,  HAISCH, Karin, University at Buffalo

IPC分类号： C07K14/435 ,  C12N15/63 ,  C07K16/28 ,  A61K38/17 ,  A61P37/00 ,  C07K1/36 ,  A61K48/00

CPC分类号： C07K14/43559 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/57 ,  Y02A50/423

摘要： The invention relates to active agents from parasitic worms, in particular Schistosoma mansoni, which induce a T-helper type 2 response (Th2 immune response).

公开(公告)号：EP0466912A1

公开(公告)日：1992-01-22

申请号：EP91904781.0

申请日：1991-02-06

申请人： TRANSGENE S.A.

发明人： TROTTEIN, François ,  CAPRON, André ,  SCHMITT, Doris ,  LECOCQ, Jean-Pierre

IPC分类号： A61K38 ,  A61P33 ,  C07K14 ,  C12N1 ,  C12N9 ,  C12N15 ,  C12R1

CPC分类号： C12N9/1088 ,  A61K38/00 ,  C07K14/43559 ,  Y02A50/423

摘要： L'invention concerne une glutathion-S-transférase de Schistosoma mansoni, ayant un poids moléculaire de 26 Kd, appelée p26 ou Sm26 ainsi que les moyens de production de cette protéine sous une forme substantiellement purifiée.

公开(公告)号：EP0211001B1

公开(公告)日：1991-06-26

申请号：EP86900214.7

申请日：1985-12-20

申请人： NATIONAL RESEARCH DEVELOPMENT CORPORATION

发明人： BENNETT, Clive, Edgar

IPC分类号： A61K39/00 ,  G01N33/53

CPC分类号： C07K16/18 ,  A61K39/00 ,  C07K14/43559

摘要： It has been a problem to combat the liver fluke parasite in ruminant animals such as sheep and cattle. The present invention provides antigens having specificity for the juvenile stage of the organism. They are defined as obtainable from juvenile Fasciola, preferably F. hepatica, organisms by either of two closely related methods. In both methods crude antigen is extracted from juvenile Fasciola organisms and used to raise an antiserum (by injection into rabbits for example). The antiserum is then reacted with adult Fasciola antigens. An adult antigen - adult antibody complex is formed, leaving the other components of the antiserum including juvenile specific antibodies. The juvenile specific antibodies are separated from the complex by spinning and "Millipore" filtering. This is followed either by (1) separating IgG components, including specific IgGs, e.g. on a protein A column or (2) subjecting the post-absorption antiserum to immunoelectrophoresis against crude juvenile-specific antigens, and raising an antiserum against the antigen component of this complex. After step (1) or (2), the juvenile-specific antibodies (purified IgGs or antiserum) are used to purify crude juvenile Fasciola antigens. It is possible to omit step (1), in which case post-absorption antiserum immunoglobuline of all classes are used in this step. Free antigen is liberated from the material bound to the column. The invention also includes a fragment of the antigen carrying a juvenile-specific antigenic determinant, a vaccine comprising the antigen or fragment thereof together with an adjuvant or carrier, and monoclonal and polyclonal antibodies to the antigen or antigenic determinant.

公开(公告)号：EP2407545A1

公开(公告)日：2012-01-18

申请号：EP10750256.9

申请日：2010-03-10

申请人： Fundação Oswaldo Cruz

发明人： TENDLER, Miriam ,  VILAR, Mônica Magno ,  ARMÔA, Geraldo Rodrigues Garcia ,  MCINTOSH, Douglas ,  SIMPSON, Andrew, J., G.

IPC分类号： C12N15/76 ,  A61P33/10 ,  A61P31/06 ,  A61P35/00

CPC分类号： A61K35/74 ,  A61K39/0003 ,  A61K39/002 ,  A61K39/04 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/43559 ,  Y02A50/423

摘要： The present invention relates to a vaccinal strain of recombinant BCG Mycobacterium bovis expressing the Schistossoma mansoni Sm14 protein (BCG Pasteur ΔleuD/pΔK410-hsp60*-Sm14). The vaccinal strain of the present invention is employed in the control of infections caused by parasites, specially the Schistossoma mansoni. The vaccinal strain is a an auxotrophic strain to leucine amino acid derived from the BCG Pasteur sub-strain complemented to leucine after genetic transformation with the construct pΔK410-hsp60*-Sm14. The efficacy of the BCG Pasteur ΔleuD/pΔK410-hsp60*-Sm14 strain in the control of the Schistossoma mansoni infection based on the recombinant Sm14 antigen in vivo is showed in the present invention.

摘要翻译： 本发明涉及重组BCG牛分枝杆菌表达Schistossoma曼氏SM14蛋白（BCG巴斯德“leuD / P” K410-HSP60 * -Sm14）的疫苗菌株。 本发明的疫苗菌株被用在感染由寄生虫引起的，特别是Schistossoma曼氏血吸虫的控制。 该疫苗菌株是营养缺陷型的，从与所述构建体P“K410-HSP60 * -Sm14遗传转化后补充到亮氨酸的BCG巴斯德子菌株的亮氨酸的氨基酸的菌株。 该BCG巴斯德“leuD / P” K410-HSP60 * -Sm14应变在基于体内重组SM14抗原Schistossoma曼氏血吸虫感染的控制的效果在本发明中被表现为。

公开(公告)号：EP1592796B1

公开(公告)日：2011-11-09

申请号：EP04706581.8

申请日：2004-01-30

申请人： FUNDACAO OSWALDO CRUZ (FIOCRUZ)

发明人： TENDLER, Miriam ,  KATZ, Naftale ,  SIMPSON, Andrew, J. ,  RAW, Isaias ,  HO, Paulo, Lee ,  RAMOS, Celso, Raul, Romero

IPC分类号： C12N15/12 ,  A61K38/17 ,  A61K39/00 ,  A61K39/39

CPC分类号： C07K14/43559 ,  A61K38/00 ,  A61K39/00 ,  G01N33/56905

摘要： The primary objective of the present invention is the development of new mutant forms of the Sm14 protein, for producing a greater production volume. The recombinant proteins here obtained were capable of providing protection against schistosome and fasciola infection. The level of protection of Sml4 recombinant proteins obtained in the present invention was similar to that reached in the parasite saline extract. The mutant proteins of the present invention have reached approximately 100% of renaturation after the heating at 80°C, different from wild forms of the Sm14 protein. Moreover, after storage for 2 months at 4°C, mutant proteins have shown smaller (3-structure loss than wild forms that have shown formation with random structure, as demonstrated by the circular dichroism analysis, indicating the success of mutations.

公开(公告)号：EP0992582A3

公开(公告)日：2003-03-26

申请号：EP99307832.8

申请日：1999-10-05

申请人： University of Wales, Bangor

发明人： Doenhoff, Michael ,  Sayers, Jon, Univ. Div. of Molecular and Gen. Med.

IPC分类号： C12N15/09 ,  A61K39/00 ,  C12N15/62

CPC分类号： C12N9/1088 ,  A61K39/00 ,  C07K14/43559 ,  C07K2319/00 ,  Y02A50/423

摘要： A vaccine for eliciting immunity against Schistosoma parasites, comprises a recombinant fusion protein capable of comprising the 27/28 kDa cercarial elastase sequence ofS. mansoni or an active fragment, homologue or variant thereof, fused to a suitable bacterial, phage or viral protein, together with a pharmaceutically acceptable excipient or carrier. The vaccine can be used to combat S. mansoni, S. japonicum and/or S. haematobium in mammals, especially humans.

公开(公告)号：EP0554064A1

公开(公告)日：1993-08-04

申请号：EP93300573.8

申请日：1993-01-27

申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD.

发明人： Schechter, Israel ,  Markovics, Alexander ,  Ram, Daniela

IPC分类号： A61K39/00 ,  C12N15/62 ,  C07K15/08 ,  C07K13/00 ,  C07H21/04 ,  A61K39/395 ,  C12N15/63 ,  G01N33/566 ,  G01N33/68

CPC分类号： C07K14/43559 ,  A61K39/00 ,  C07K2319/35 ,  C07K2319/40 ,  Y02A50/423

摘要： An immunogenically and antigenically active protein capable of eliciting immunity against Schistosoma parasites is provided, as are vaccines containing the protein, that are capable of eliciting in humans or animals an immune response that affords protection against infection by Schistosoma parasites. Antibodies raised against the protein, DNA molecules encoding the protein, vectors containing the DNA molecules, host cells transformed by the vectors, and diagnostic methods using the antibodies or the proteins are also provided.

摘要翻译： 提供了能够引起针对血吸虫寄生虫免疫的免疫原性和抗原活性蛋白质，以及能够在人或动物中引发免疫应答的免疫原性和含有蛋白质的疫苗，其提供了针对血吸虫寄生虫感染的免疫应答。 还提供了针对蛋白质的抗体，编码蛋白质的DNA分子，含有DNA分子的载体，由载体转化的宿主细胞，以及使用抗体或蛋白质的诊断方法。

公开(公告)号：EP0268501A1

公开(公告)日：1988-05-25

申请号：EP87401915.1

申请日：1987-08-20

申请人： TRANSGENE S.A.

发明人： Sondermeyer, Paul ,  Balloul, Jean-Marc ,  Pierce, Raymond ,  Grzych, Jean-Marie ,  Kieny, Marie-Paule ,  Loison, Gérard ,  Capron, André ,  Lecocq, Jean-Pierre

IPC分类号： C12N15/00 ,  C07K13/00 ,  C12N1/20 ,  C12N7/00 ,  C12N5/00 ,  C12P21/02 ,  A61K37/02 ,  C07K15/00 ,  C12N9/10 ,  A61K39/00

CPC分类号： C12N15/86 ,  A61K39/00 ,  C07K14/005 ,  C07K14/4354 ,  C07K14/43559 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/1088 ,  C12N9/12 ,  C12N15/73 ,  C12N15/81 ,  C12N2710/24143 ,  C12N2760/20122 ,  Y02A50/423

摘要： La présente invention vise à la mise au point d'un vaccin contre la schistosomose. Elle concerne une protéine comprenant les épitopes de la protéine p28, un poxvirus comportant un gène codant pour ladite protéine, une cellule incorporant un vecteur d'expression de ladite protéine, un procédé de préparation de ladite protéine, une séquence d'ADN codant pour la protéine p28, une composition pharmaceutique, des anticorps dressés contre ladite protéine et leur application à titre d'agents de diagnostic de la schistosomose.
La présente invention concerne aussi l'application de ladite protéine à titre d'agent présentant une activité glutathion-S-transferase.

摘要翻译： 本发明是在开发针对schistosomosis疫苗瞄准。 本发明涉及包含所述蛋白P28的表位的蛋白质，痘病毒包含基因编码所述蛋白质，所述的蛋白质的表达载体的细胞结合，为对所述蛋白质的制备方法，DNA序列编码 蛋白质P28，药物组合物，竖起抗体针对该蛋白质。所述及其作为schistosomosis诊断试剂的应用。 因此，本发明涉及所述蛋白质的应用作为代理的表现出谷胱甘肽-S-转移酶活性。

公开(公告)号：EP2617829A1

公开(公告)日：2013-07-24

申请号：EP11824375.7

申请日：2011-09-13

申请人： Fundação Oswaldo Cruz ,  Alvos - Consultoria, Desenvolvimento E Comercializ

发明人： TENDLER, Miriam ,  RAMOS, Celso Raul Romero Ramos ,  SIMPSON, Andrew, J.G.

IPC分类号： C12N15/12 ,  C12N15/63 ,  C12N15/79 ,  A61K38/17 ,  A61K39/17

CPC分类号： C07K1/36 ,  A61K38/00 ,  A61K39/0003 ,  C07K14/395 ,  C07K14/43559 ,  C12N15/815 ,  Y02A50/423

摘要： The present invention is related to the field of recombinant protein production using a synthetic gene associated with high protein expression in Pichia pastoris. More specifically, this invention describes the production of Sm14 Schistosoma mansoni recombinant protein. A synthetic gene was created to promote high Sm14 expression, and with this gene we obtained and genetically manipulated a Pichia pastoris strain for effectively producing a vaccine. We have also improved that protein's production and purification processes from P. pastoris cells; the industrial production of such processes may be scheduled.

摘要翻译： 本发明涉及使用具有在毕赤酵母的高蛋白质表达相关的合成基因的重组蛋白生产的领域。 更具体地说，本发明描述了生产重组蛋白SM14曼氏血吸虫的。 这创造，以促进高SM14表达，并用该基因我们获得和基因的合成基因操纵的巴斯德毕赤酵母株为有效生产疫苗。 因此，我们必须改善并从毕赤酵母细胞中蛋白质的生产和纯化过程; 工业生产检索处理可以被调度。