公开(公告)号：EP4352521A1

公开(公告)日：2024-04-17

申请号：EP22820916.9

申请日：2022-06-07

申请人： Martino, Michael, L.

发明人： Martino, Michael, L.

IPC分类号： G01N33/68 ,  G01N33/558

CPC分类号： G01N33/54388 ,  A61K2039/50520130101 ,  A61P31/04 ,  C07K16/1232 ,  C07K2317/5520130101

公开(公告)号：EP3374391A2

公开(公告)日：2018-09-19

申请号：EP16806339.4

申请日：2016-11-10

申请人： Visterra, Inc.

发明人： WOLLACOTT, Andrew, M. ,  PLANTE, Obadiah, Joseph ,  DELANEY, James, C. ,  VISWANATHAN, Karthik ,  RAMAKRISHNAN, Boopathy ,  SHRIVER, Zachary, Holmes

IPC分类号： C07K16/12 ,  C07K16/44 ,  C07K7/00 ,  A61K47/00

CPC分类号： C07K16/1232 ,  A61K38/10 ,  A61K38/16 ,  A61K39/40 ,  A61K45/06 ,  A61K47/6811 ,  A61K47/6835 ,  A61K2039/505 ,  C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C07K16/1203 ,  C07K16/1214 ,  C07K16/1228 ,  C07K16/1235 ,  C07K16/44 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/00 ,  Y02A50/473 ,  Y02A50/475 ,  Y02A50/484

摘要： Antibody molecule-drug conjugates (ADCs) that specifically bind to lipopolysaccharides (LPS) are disclosed. The antibody molecule-drug conjugates can be used to treat, prevent, and/or diagnose bacterial infections and related disorders.

公开(公告)号：EP3311661A1

公开(公告)日：2018-04-25

申请号：EP17196235.0

申请日：2012-09-18

申请人： Kymab Limited

发明人： FRIEDRICH, Glenn A ,  LEE, E-Chiang ,  CLUBE, Jasper ,  ENGLAND, Nicholas

IPC分类号： A01K67/027 ,  C12N15/85 ,  C07K16/46

CPC分类号： C07K16/1242 ,  A01K67/0278 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/085 ,  C07K16/088 ,  C07K16/1018 ,  C07K16/1045 ,  C07K16/1217 ,  C07K16/1232 ,  C07K16/18 ,  C07K16/46 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/56 ,  C12N15/8509 ,  C12N2800/204

摘要： The invention provides improved non-human vertebrates and non-vertebrate cells capable of expressing antibodies comprising human variable region sequences. The present invention is directed to the provision of long HCDR3s from non-human vertebrates and cells. The present invention is also directed to the provision of novel V, D and J pairings in immunoglobulin heavy and light chain loci. Novel, biased antibody diversities and potentially expanded diversities are provided. The invention also provides for novel and potentially expanded diversity or diversity that is biased towards variable gene usage common to antibodies useful for treating and/or preventing certain diseases or conditions, such as infectious diseases. The invention also provides methods of generating antibodies using such vertebrates, as well as the antibodies per se, therapeutic compositions thereof and uses.

摘要翻译： 本发明提供了能够表达包含人可变区序列的抗体的改良的非人脊椎动物和非脊椎动物细胞。 本发明涉及提供来自非人类脊椎动物和细胞的长HCDR3。 本发明还涉及在免疫球蛋白重链和轻链基因座中提供新的V，D和J配对。 提供了新颖，有偏见的抗体多样性和潜在的扩展多样性。 本发明还提供了新的和可能扩展的多样性或多样性，其偏向于可用于治疗和/或预防某些疾病或病症（例如感染性疾病）的抗体常见的可变基因用途。 本发明还提供了使用这种脊椎动物产生抗体的方法，以及抗体本身，其治疗组合物和用途。

公开(公告)号：EP3189075A1

公开(公告)日：2017-07-12

申请号：EP15759793.1

申请日：2015-09-04

申请人： Laboratoire Français du Fractionnement et des Biotechnologies

发明人： COUTARD, François

IPC分类号： C07K16/06 ,  C07K1/36 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34

CPC分类号： B01D15/3809 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34 ,  C07K1/36 ,  C07K16/065 ,  C07K16/08 ,  C07K16/12 ,  C07K16/1232 ,  C07K16/1271 ,  C07K16/1282 ,  C07K2317/76 ,  C07K2319/30 ,  C12M47/12 ,  C12N15/8258 ,  G01N30/02

摘要： The present invention relates to a method for purification of monoclonal antibodies or of a fusion protein between the Fc segment of an antibody and a second polypeptide, comprising a) an affinity chromatography step on a resin having as a matrix a crosslinked methacrylate polymer gel, on which the protein A is grafted, b) a viral inactivation step, c) a chromatography step exchanging cations on a resin having a crosslinked agarose gel matrix, on which sulfonate groups (-SO
3 -) are grafted using dextran-based spacer arms, d) a chromatography step exchanging anions on a hydrophilic membrane of polyethersulfone coated with a crosslinked polymer on which quaternary amine groups (Q)are grafted, and e) a nanofiltration step using a filter having an asymmetric polyethersulfone double membrane with a porosity of approximately 20 nm.

摘要翻译： 本发明涉及用于纯化抗体的Fc片段和第二多肽之间的单克隆抗体或融合蛋白的方法，其包括a）在具有作为基质的交联的甲基丙烯酸酯聚合物凝胶的树脂上的亲和层析步骤， 其中蛋白质A被接枝，b）病毒灭活步骤，c）在具有交联琼脂糖凝胶基质的树脂上交换阳离子的层析步骤，其上使用基于葡聚糖的间隔臂接枝磺酸盐基团（-SO 3 - ），d ）色层分离步骤，交换聚醚砜的亲水膜上的阴离子，所述聚醚砜涂覆有接枝有季胺基团（Q）的交联聚合物，和e）使用具有孔径约20nm的不对称聚醚砜双膜的过滤器的纳滤步骤 。

公开(公告)号：EP3182997A1

公开(公告)日：2017-06-28

申请号：EP15834013.3

申请日：2015-08-24

申请人： Nectagen Inc.

发明人： SUDERMAN, Richard J. ,  CHAO, David M. ,  RICE, Daren

IPC分类号： A61K39/00 ,  C12P21/08 ,  C07K16/12

CPC分类号： C07K16/4283 ,  C07K16/00 ,  C07K16/1228 ,  C07K16/1232 ,  C07K16/18 ,  C07K16/40 ,  C07K2318/20 ,  C12N15/1044

摘要： In general, the invention features a novel CBM32-derived affinity scaffold. In certain embodiments, the scaffold comprises two types of regions: constant regions (CRs) and variable loop regions (VLRs) as depicted in the structure in FIG. 1. We have discovered that the CRs provide structural features that enable overall conformational stability while the intervening sequences corresponding to VLRs tolerate amino acid sequence randomization.

摘要翻译： 一般而言，本发明具有新颖的CBM32衍生亲和支架。 在某些实施方案中，支架包含两类区域：如图1中的结构所示​​的恒定区（CR）和可变环区（VLR）。我们已经发现CRs提供了结构特征，该结构特征能够实现总体构象稳定性，同时介入 对应于VLR的序列耐受氨基酸序列随机化。

公开(公告)号：EP2381954B1

公开(公告)日：2016-11-02

申请号：EP10733840.2

申请日：2010-01-21

申请人： The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.

发明人： O'BRIEN, Alison ,  MELTON-CELSA, Angela ,  SMITH, Michael ,  SINCLAIR, James

IPC分类号： A61K38/00 ,  C07K14/00

CPC分类号： A61K39/0283 ,  A61K38/00 ,  A61K39/0258 ,  A61K2039/505 ,  C07K7/06 ,  C07K7/08 ,  C07K14/25 ,  C07K16/1232 ,  C07K2317/34 ,  Y02A50/473

摘要： The invention is based on the discovery of the epitope in the Stx2 protein for the 11 E1O antibody. The invention features compositions containing non-full length Stx2 polypeptides that include the 11 E1O monoclonal antibody epitope. The invention also features methods of producing anti-Stx2 antibodies specific for the 11 E1O epitope of the Stx2 protein. Additionally, the invention features methods for treating a subject having, or at risk of developing, a Shiga toxin associated disease (e.g., hemolytic uremia syndrome and diseases associated with E. coli and S. dysenteriae infection) with a polypeptide that includes the 11 E1O epitope or with an anti-Stx2 antibody developed using the methods of the invention. Furthermore, the invention features the detection of Stx2 in a sample using the antibodies developed using the methods of the invention.

公开(公告)号：EP2417165B1

公开(公告)日：2016-05-25

申请号：EP10761153.5

申请日：2010-04-06

申请人： UNIVERSITY OF SASKATCHEWAN

发明人： POTTER, Andrew, A. ,  ASPER, David ,  ROGAN, Dragan

IPC分类号： C07K19/00 ,  A61K39/108 ,  A61K39/385 ,  A61P31/04 ,  A61P37/04 ,  C07K14/245

CPC分类号： A61K39/0258 ,  A61K2039/552 ,  A61K2039/55566 ,  A61K2039/6037 ,  C07K16/1232 ,  Y02A50/474

摘要： Compositions and methods for stimulating an immune response against Shiga toxin-producing Escherichia coli (STEC) antigens are disclosed. The compositions include a multiple epitope fusion protein comprising more than one epitope of an immunogenic STEC protein from more than one STEC serotype. Additional compositions include at least two purified STEC proteins, wherein the STEC proteins are selected from a full-length STEC protein, an immunogenic fragment or variant thereof, wherein at least one of the STEC proteins generates antibodies that react with STEC O157 and at least one other STEC serotype.

公开(公告)号：EP3003362A1

公开(公告)日：2016-04-13

申请号：EP14738894.6

申请日：2014-06-04

申请人： Wroclawskie Centrum Badan EIT + Sp. z o.o.

发明人： GOZDZIEWICZ, Tomasz ,  LUKASIEWICZ, Jolanta ,  LUGOWSKI, Czeslaw

IPC分类号： A61K39/02 ,  C08B37/00

CPC分类号： A61K47/4823 ,  A61K39/025 ,  A61K39/0258 ,  A61K39/0266 ,  A61K39/0275 ,  A61K39/0283 ,  A61K39/39 ,  A61K47/61 ,  A61K2039/55572 ,  A61K2039/6037 ,  C07K16/1228 ,  C07K16/1232 ,  C07K16/1235 ,  C07K2317/76 ,  C08B37/00 ,  Y02A50/476

摘要： The subject of the present invention is an isolated antigen being an immunogenic form of the common enterobacterial antigen (ECA) of Gram-negative bacteria of the family Enterobacteriaceae: ECA combined with lipopolysaccharide (ECALPS); a glycoconjugate of inactivated form of this antigen with a protein, as well as compositions and vaccines containing such an antigen/glycoconjugate designed for the prevention and treatment infections caused by Gram-negative bacteria.

公开(公告)号：EP2972367A1

公开(公告)日：2016-01-20

申请号：EP14709835.4

申请日：2014-02-24

申请人： Silver Lake Research Corporation

发明人： DINELLO, Robert K. ,  GEISBERG, Mark

IPC分类号： G01N33/569 ,  A61K39/40 ,  C07K16/12 ,  G01N33/68

CPC分类号： G01N33/56911 ,  C07K16/1203 ,  C07K16/1232 ,  C07K2317/30 ,  C07K2317/34 ,  G01N33/56916 ,  G01N33/56922 ,  G01N33/56927 ,  G01N33/56933 ,  G01N33/56938 ,  G01N33/56944 ,  G01N33/5695 ,  G01N33/6854

摘要： In some aspects, provided are methods relating to the use of bacterial N-methyl-2 superfamily proteins as a biomarker for the presence of bacteria in a sample. The invention also relates to novel methods of diagnosis of the presence of bacteria in a liquid or solid sample, detection of bacterial infections in humans or animals, and use of antibodies or other specific binding molecules capable of binding to N-methyl-2 superfamily proteins.

摘要翻译： 在某些方面，所提供的方法涉及使用细菌N-甲基-2超家族蛋白作为样品中细菌存在的生物标志物。 本发明还涉及诊断液体或固体样品中细菌存在的新方法，检测人或动物中的细菌感染，以及使用能够结合N-甲基-2超家族蛋白的抗体或其它特异性结合分子 。

公开(公告)号：EP2328927B1

公开(公告)日：2015-12-23

申请号：EP09752002.7

申请日：2009-09-27

申请人： Instytut Immunologii i Terapii Doswiadczalnej Polskiej Akademii Nauk

发明人： LIPINSKI, Tomasz ,  RYBKA, Jacek ,  GAMIAN, Andrzej

IPC分类号： C08B37/00 ,  C07K16/12

CPC分类号： C07K16/1232 ,  C08B37/0003