公开(公告)号：EP2942391B1

公开(公告)日：2018-05-23

申请号：EP15167706.9

申请日：2005-08-15

申请人： The Trustees of The University of Pennsylvania

发明人： Paterson, Yvonne ,  Verch, Thorsten

IPC分类号： C12N1/21 ,  C12N15/74 ,  C12N9/10 ,  C12N9/90 ,  A61K35/74

CPC分类号： A61K39/0011 ,  A61K35/74 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/585 ,  C12N7/00 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N2710/00034 ,  C12N2710/20033 ,  C12Y206/01 ,  C12Y206/01021 ,  C12Y501/01001

摘要： A method of engineering a recombinant Listeria vaccine strain to express an HPV antigen, comprising: contacting a Listeria strain auxotrophic for D-alanine synthesis comprising mutations in a D-alanine racemase and a D-amino acid transferase gene in the Listeria's chromosome with a plasmid, the plasmid comprising a first nucleic acid sequence encoding a polypeptide that comprises a human papilloma virus E7 antigen, and a second nucleic acid sequence encoding a D-alanine racemase, wherein the plasmid does not confer antibiotic resistance to the auxotrophic Listeria vaccine strain, whereby the auxotrophic Listeria strain takes up the plasmid and the D-alanine racemase complements the mutation in the D-alanine racemase gene, wherein the auxotrophic Listeria strain grows in the absence of D-alanine, and wherein the first nucleic acid sequence is operably linked to a prokaryotic promoter, thereby engineering a recombinant Listeria vaccine strain to express the HPV antigen.

公开(公告)号：EP3046583A1

公开(公告)日：2016-07-27

申请号：EP14845738.5

申请日：2014-09-18

申请人： Aura Biosciences Inc. ,  The United States Of America, As Represented By The Secretary, Department Of Health And Human Services

发明人： DE LOS PINOS, Elisabet ,  SCHILLER, John, T. ,  KINES, Rhonda, C. ,  MACDOUGALL, John

IPC分类号： A61K39/42

CPC分类号： A61K47/6901 ,  A61K39/12 ,  A61K41/0057 ,  A61K41/0071 ,  A61K47/64 ,  A61K2039/545 ,  A61K2039/585 ,  A61N5/062 ,  C07K16/084 ,  C07K16/32 ,  C07K2317/33 ,  C07K2317/76 ,  C12N7/00 ,  C12N2710/20023 ,  C12N2710/20033 ,  C12N2710/20034

摘要： The present disclosure is directed to methods and compositions for the diagnosis and/or treatment of tumors, such as ocular tumors, using virus-like particles conjugated to photosensitive molecules.

公开(公告)号：EP3046583A4

公开(公告)日：2017-05-03

申请号：EP14845738

申请日：2014-09-18

申请人： AURA BIOSCIENCES INC ,  US HEALTH

发明人： DE LOS PINOS ELISABET ,  SCHILLER JOHN T ,  KINES RHONDA C ,  MACDOUGALL JOHN

IPC分类号： A61K39/42 ,  C07K16/32

CPC分类号： A61K47/48246 ,  A61K39/12 ,  A61K41/0057 ,  A61K47/64 ,  A61K2039/545 ,  A61K2039/585 ,  A61N5/062 ,  C07K16/084 ,  C07K16/32 ,  C07K2317/33 ,  C07K2317/76 ,  C12N7/00 ,  C12N2710/20023 ,  C12N2710/20033 ,  C12N2710/20034

摘要： The present disclosure is directed to methods and compositions for the diagnosis and/or treatment of tumors, such as ocular tumors, using virus-like particles conjugated to photosensitive molecules.

摘要翻译： 本公开涉及使用缀合至光敏分子的病毒样颗粒来诊断和/或治疗肿瘤（例如眼部肿瘤）的方法和组合物。

公开(公告)号：EP2942391A1

公开(公告)日：2015-11-11

申请号：EP15167706.9

申请日：2005-08-15

申请人： The Trustees of The University of Pennsylvania

发明人： Paterson, Yvonne ,  Verch, Thorsten

IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N9/10 ,  C12N9/90 ,  A61K39/12

CPC分类号： A61K39/0011 ,  A61K35/74 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/585 ,  C12N7/00 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N2710/00034 ,  C12N2710/20033 ,  C12Y206/01 ,  C12Y206/01021 ,  C12Y501/01001

摘要： A method of engineering a recombinant Listeria vaccine strain to express an HPV antigen, comprising: contacting a Listeria strain auxotrophic for D-alanine synthesis comprising mutations in a D-alanine racemase and a D-amino acid transferase gene in the Listeria's chromosome with a plasmid, the plasmid comprising a first nucleic acid sequence encoding a polypeptide that comprises a human papilloma virus E7 antigen, and a second nucleic acid sequence encoding a D-alanine racemase, wherein the plasmid does not confer antibiotic resistance to the auxotrophic Listeria vaccine strain, whereby the auxotrophic Listeria strain takes up the plasmid and the D-alanine racemase complements the mutation in the D-alanine racemase gene, wherein the auxotrophic Listeria strain grows in the absence of D-alanine, and wherein the first nucleic acid sequence is operably linked to a prokaryotic promoter, thereby engineering a recombinant Listeria vaccine strain to express the HPV antigen.

公开(公告)号：EP3350337A1

公开(公告)日：2018-07-25

申请号：EP16847136.5

申请日：2016-09-13

申请人： Advaxis, Inc. ,  The Trustees of the University of Pennsylvania

发明人： WALLECHA, Anu ,  PATERSON, Yvonne

IPC分类号： C12P21/00 ,  C12N15/74 ,  C07K14/005 ,  C07K14/195 ,  A61K38/16

CPC分类号： C12N15/74 ,  A61K35/74 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/585 ,  A61K2039/6068 ,  A61K2039/6075 ,  A61K2039/892 ,  A61P35/00 ,  C07K14/005 ,  C07K14/025 ,  C07K14/195 ,  C07K19/00 ,  C07K2319/06 ,  C07K2319/55 ,  C12N1/20 ,  C12N5/00 ,  C12N2330/50 ,  C12N2511/00 ,  C12N2710/20022 ,  C12N2710/20033 ,  C12N2710/20034 ,  C12N2710/20043 ,  C12N2710/20051 ,  C12N2740/16234 ,  C12N2800/101 ,  C12P21/00

摘要： The present invention discloses a process for manufacturing a formulation comprising a drug substance, said drug substance comprising a recombinant Listeria comprising a human papilloma virus (HPV) antigen fused to a Listeriolysin O (LLO) protein fragment. The invention further discloses methods of using treating, protecting against, and inducing an immune response against cervical cancer comprising administration of the recombinant Listeria strain. The present invention also provides methods for inducing an anti-E7 CTL response in a human subject and treating HPV-mediated diseases, disorders, and symptoms, comprising administration of the recombinant Listeria strain.

公开(公告)号：EP3261667A1

公开(公告)日：2018-01-03

申请号：EP16756299.0

申请日：2016-02-24

申请人： TheVax Genetics Vaccine Co., Ltd.

发明人： WU, Chia-Mao ,  WU, Jiun-Ming ,  CHIU, Yi-Tsui ,  LIN, Yin-Ching ,  CHUANG, Hsien-Kai ,  HSIEH, Fu-Tan ,  CHEN, Kuan-Ming

IPC分类号： A61K39/21 ,  A61K47/50

CPC分类号： A61K39/39 ,  A61K38/164 ,  A61K38/1774 ,  A61K39/12 ,  A61K2039/545 ,  A61K2039/55511 ,  A61K2039/55561 ,  A61K2039/55572 ,  A61K2039/55577 ,  A61K2039/585 ,  A61K2039/6031 ,  A61K2039/6037 ,  C12N7/00 ,  C12N2710/20033 ,  C12N2710/20034 ,  C12N2710/20071 ,  Y02A50/386

摘要： Vaccine compositions for use in inducing enhanced antigen-specific T cell-mediated immune responses in a subject in need thereof are disclosed. The composition comprises (a) a therapeutically effective amount of an immunogenic protein comprising at least an antigen of a pathogen; (b) a saponin-base adjuvant selected from the group consisting of GPI-0100, Quil A, QS-21; and (c) a Toll-like receptor (TLR) agonist adjuvant selected from the group consisting of monophosphoryl lipid A (MPL), and CpG1826.

公开(公告)号：EP2968500A4

公开(公告)日：2016-06-29

申请号：EP14767446

申请日：2014-03-13

申请人： UNIV PENNSYLVANIA

发明人： WEINER DAVID ,  MORROW MATTHEW ,  YAN JIAN ,  FERRARO BERNADETTE ,  HOKEY DAVID

IPC分类号： A61K39/00

CPC分类号： A61K39/39 ,  A61K9/0009 ,  A61K39/015 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55527 ,  C07K14/54 ,  C12N7/00 ,  C12N15/03 ,  C12N2710/20033 ,  C12N2710/20034 ,  C12P21/02

摘要： Disclosed herein is a vaccine comprising an antigen and IL-23. Also disclosed herein are methods for increasing an immune response in a subject. The methods may comprise administering the vaccine to the subject in need thereof.

公开(公告)号：EP2968500A1

公开(公告)日：2016-01-20

申请号：EP14767446.9

申请日：2014-03-13

申请人： The Trustees Of The University Of Pennsylvania

发明人： WEINER, David ,  MORROW, Matthew ,  YAN, Jian ,  FERRARO, Bernadette ,  HOKEY, David

IPC分类号： A61K39/00

CPC分类号： A61K39/39 ,  A61K9/0009 ,  A61K39/015 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55527 ,  C07K14/54 ,  C12N7/00 ,  C12N15/03 ,  C12N2710/20033 ,  C12N2710/20034 ,  C12P21/02 ,  Y02A50/412

摘要： Disclosed herein is a vaccine comprising an antigen and IL-23. Also disclosed herein are methods for increasing an immune response in a subject. The methods may comprise administering the vaccine to the subject in need thereof.

摘要翻译： 本文公开了包含抗原和IL-23的疫苗。 本文还公开了增加受试者的免疫应答的方法。 所述方法可以包括向有需要的受试者施用疫苗。

公开(公告)号：EP1789559B1

公开(公告)日：2015-05-20

申请号：EP05808671.1

申请日：2005-08-15

申请人： The Trustees of The University of Pennsylvania

发明人： PATERSON, Yvonne ,  VERCH, Thorsten

IPC分类号： C12N15/68 ,  C12N1/20 ,  A61K39/00 ,  C12N15/65 ,  C12N15/74 ,  A61K35/74 ,  C12N15/61

CPC分类号： A61K39/0011 ,  A61K35/74 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/585 ,  C12N7/00 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N2710/00034 ,  C12N2710/20033 ,  C12Y206/01 ,  C12Y206/01021 ,  C12Y501/01001

摘要： A method of engineering a recombinant Listeria vaccine strain to express an HPV antigen, comprising: contacting a Listeria strain auxotrophic for D-alanine synthesis comprising mutations in a D-alanine racemase and a D-amino acid transferase gene in the Listeria's chromosome with a plasmid, the plasmid comprising a first nucleic acid sequence encoding a polypeptide that comprises a human papilloma virus E7 antigen, and a second nucleic acid sequence encoding a D-alanine racemase, wherein the plasmid does not confer antibiotic resistance to the auxotrophic Listeria vaccine strain, whereby the auxotrophic Listeria strain takes up the plasmid and the D-alanine racemase complements the mutation in the D-alanine racemase gene, wherein the auxotrophic Listeria strain grows in the absence of D-alanine, and wherein the first nucleic acid sequence is operably linked to a prokaryotic promoter, thereby engineering a recombinant Listeria vaccine strain to express the HPV antigen.

公开(公告)号：EP1789559A4

公开(公告)日：2009-02-11

申请号：EP05808671

申请日：2005-08-15

申请人： UNIV PENNSYLVANIA

发明人： PATERSON YVONNE ,  VERCH THORSTEN

IPC分类号： C12N15/68 ,  A61K35/74 ,  A61K39/00 ,  C12N1/20 ,  C12N15/61 ,  C12N15/65 ,  C12N15/74

CPC分类号： A61K39/0011 ,  A61K35/74 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/585 ,  C12N7/00 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N2710/00034 ,  C12N2710/20033 ,  C12Y206/01 ,  C12Y206/01021 ,  C12Y501/01001

摘要： A method of engineering a recombinant Listeria vaccine strain to express an HPV antigen, comprising: contacting a Listeria strain auxotrophic for D-alanine synthesis comprising mutations in a D-alanine racemase and a D-amino acid transferase gene in the Listeria's chromosome with a plasmid, the plasmid comprising a first nucleic acid sequence encoding a polypeptide that comprises a human papilloma virus E7 antigen, and a second nucleic acid sequence encoding a D-alanine racemase, wherein the plasmid does not confer antibiotic resistance to the auxotrophic Listeria vaccine strain, whereby the auxotrophic Listeria strain takes up the plasmid and the D-alanine racemase complements the mutation in the D-alanine racemase gene, wherein the auxotrophic Listeria strain grows in the absence of D-alanine, and wherein the first nucleic acid sequence is operably linked to a prokaryotic promoter, thereby engineering a recombinant Listeria vaccine strain to express the HPV antigen.

摘要翻译： 一种重组利斯特氏菌疫苗菌株的表达HPV抗原的方法，其特征在于包括：将具有营养缺陷型营养缺陷型营养缺陷型D-丙氨酸合成，其包含利斯特氏菌染色体中的D-丙氨酸消旋酶和D-氨基酸转移酶基因中的突变， ，所述质粒包含编码包含人乳头状瘤病毒E7抗原的多肽的第一核酸序列和编码D-丙氨酸消旋酶的第二核酸序列，其中所述质粒不赋予营养缺陷型李斯特菌属疫苗株抗生素抗性，由此 营养缺陷型李斯特氏菌菌株吸收质粒，D-丙氨酸消旋酶补充D-丙氨酸消旋酶基因中的突变，其中营养缺陷型李斯特菌属菌株在不存在D-丙氨酸的情况下生长，并且其中第一核酸序列可操作地连接到 一种原核启动子，从而工程化重组利斯特氏菌疫苗株，以表达HPV抗原。