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    RECOMBINANT PHAGE AND METHODS
    1.
    发明公开
    RECOMBINANT PHAGE AND METHODS 有权
    REKOMBINANTER PHAGE UND VERFAHREN

    公开(公告)号：EP2761014A4

    公开(公告)日：2015-04-22

    申请号：EP12835856

    申请日：2012-09-26

    申请人： SAMPLE6 TECHNOLOGIES INC

    发明人： LU TIMOTHY KUAN TA ,  KOERIS MICHAEL SANDOR ,  CHEVALIER BRETT SMITH ,  HOLDER JASON WYATT ,  MCKENZIE GREGORY JOHN ,  BROWNELL DANIEL ROBERT

    IPC分类号： C12P19/34

    CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

    摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

    摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。

    PRODUCTION OF LYSIN-FREE PHAGE INOCULUM AND PRODUCTION OF LYSIN
    6.
    发明公开
    PRODUCTION OF LYSIN-FREE PHAGE INOCULUM AND PRODUCTION OF LYSIN 失效
    生产不含赖氨酸的菌液和生产赖氨酸

    公开(公告)号：EP0324018A1

    公开(公告)日：1989-07-19

    申请号：EP88906660.0

    申请日：1988-07-22

    申请人： IGENE BIOTECHNOLOGY, INC.

    发明人： YANG, Huei-Hsiung ,  HIU, Stephen, F. ,  HARRIS, John, L.

    IPC分类号： C12N9 ,  C12N7 ,  C12R1

    CPC分类号： C12N7/00 ,  C12N9/14 ,  C12N2795/10251 ,  Y10S435/885

    摘要： A method for producing a lysin-free phage inoculum, which comprises: a) inoculating a growing Streptococcal cul­ ture with phage; b) incubating the culture for plurality of lytic cycles of phage until the cells are completely lysed to obtain a lysate; and c) removing cell debris and free lysin from the lysate to form a lysin-free phage suitable for use as an inocu­ lum. Also disclosed is a method of producing lysin.

    摘要翻译： 生产不含赖氨酸的噬菌体接种物的一种方法是：a）用噬菌体接种正在生长的链球菌培养物; b）将培养物温育几个噬菌体裂解周期，直到细胞完全裂解以获得裂解物; 和c）从裂解物中提取细胞碎片和游离赖氨酸以形成适合用作接种物的不含赖氨酸的噬菌体。 还描述了生产赖氨酸的方法。