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公开(公告)号：EP3011062A2
公开(公告)日：2016-04-27
申请号：EP14813675.7
申请日：2014-06-19
发明人： KOERIS, Michael Sandor , THOMASON, Julie , CAPPILLINO, Michael , SHIVERS, Robert Patrick , BROWNELL, Daniel Robert , BOWERS, Jayson L. , LU, Timothy Kuan Ta , KRZYMANSKA-OLEJNIK, Edyta
IPC分类号： C12Q1/70
CPC分类号： C12Q1/66 , C12N7/00 , C12Q1/10 , G01N33/82 , G01N2333/245 , G01N2333/255
摘要： Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
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公开(公告)号：EP3320108A1
公开(公告)日：2018-05-16
申请号：EP16824938.1
申请日：2016-07-08
发明人： KOERIS, Michael Sandor , CAPPILLINO, Michael , KRZYMANSKA-OLEJNIK, Edyta , BROWNELL, Daniel Robert , SHIVERS, Robert Patrick , BOWERS, Jayson L. , LU, Timothy Kuan-Ta
CPC分类号： C12Q1/04 , C12N7/00 , C12N15/86 , C12N2795/00022 , C12N2795/00043
摘要： Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is Listeria.
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公开(公告)号：EP2761014A4
公开(公告)日：2015-04-22
申请号：EP12835856
申请日：2012-09-26
发明人： LU TIMOTHY KUAN TA , KOERIS MICHAEL SANDOR , CHEVALIER BRETT SMITH , HOLDER JASON WYATT , MCKENZIE GREGORY JOHN , BROWNELL DANIEL ROBERT
IPC分类号： C12P19/34
CPC分类号： C12N7/00 , C12N15/10 , C12N15/81 , C12N15/86 , C12N2795/10143 , C12N2795/10221 , C12N2795/10243 , C12N2795/10251 , C12N2800/206 , C12P19/34
摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。
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公开(公告)号：EP2883154A4
公开(公告)日：2016-01-27
申请号：EP13828124
申请日：2013-08-09
摘要： Methods and systems are provided herein for monitoring pathogens in various environments and on various items, wherein data from monitoring is trackable, analyzable and comparable versus various standards or thresholds. The methods and systems disclosed herein also include a platform for managing the detection and reporting of pathogens across a number of locations within a number of environments, and using such detection for a wide variety of purposes.
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公开(公告)号：EP2883154A1
公开(公告)日：2015-06-17
申请号：EP13828124.1
申请日：2013-08-09
摘要： Methods and systems are provided herein for monitoring pathogens in various environments and on various items, wherein data from monitoring is trackable, analyzable and comparable versus various standards or thresholds. The methods and systems disclosed herein also include a platform for managing the detection and reporting of pathogens across a number of locations within a number of environments, and using such detection for a wide variety of purposes.
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公开(公告)号：EP2761014A2
公开(公告)日：2014-08-06
申请号：EP12835856.1
申请日：2012-09-26
发明人： LU, Timothy, Kuan Ta , KOERIS, Michael, Sandor , CHEVALIER, Brett, Smith , HOLDER, Jason, Wyatt , MCKENZIE, Gregory, John , BROWNELL, Daniel, Robert
IPC分类号： C12P19/34
CPC分类号： C12N7/00 , C12N15/10 , C12N15/81 , C12N15/86 , C12N2795/10143 , C12N2795/10221 , C12N2795/10243 , C12N2795/10251 , C12N2800/206 , C12P19/34
摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
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