CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    2.
    发明公开
    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 审中-公开
    纤维素 - 发光/光合细胞藻类化合物大肠杆菌

    公开(公告)号:EP2885406A4

    公开(公告)日:2016-04-27

    申请号:EP13829732

    申请日:2013-08-15

    IPC分类号: C12N9/42 C12N15/00

    摘要: The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

    摘要翻译: 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina(“M. phaseolina”)的核苷酸序列,包括纤维素酶活性,内切葡聚糖酶,纤维二糖水解酶,α-葡糖苷酶,α-葡糖苷酶,木聚糖酶, 甘露聚糖,α-木糖苷酶,α-木糖苷酶,半乳糖苷酶,阿拉伯呋喃糖苷酶,α-岩藻糖苷酶,β-半乳糖苷酶,不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 还提供了载体,表达构建体和包含酶基因的核苷酸序列的和/或由其组成的宿主细胞。 本发明还提供了用于生产酶的方法和用于修饰酶的方法,以便改善其期望的特性。 本发明的酶可以多种但不限于制药,农业,食品和饲料加工,生物燃料,能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成简单的糖,然后将其发酵成液体燃料和化学原料。

    COMPOSITIONS AND METHODS OF USE
    4.
    发明公开
    COMPOSITIONS AND METHODS OF USE 有权
    组合物和使用方法

    公开(公告)号:EP2929023A1

    公开(公告)日:2015-10-14

    申请号:EP13811687.6

    申请日:2013-12-02

    申请人: Danisco US Inc.

    IPC分类号: C12N9/24

    摘要: The present compositions and methods relate to a beta-mannanase from Thermobifida fusca, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

    摘要翻译: 本发明的组合物和方法涉及来自Thermobifida fusca的β-甘露聚糖酶,编码β-甘露聚糖酶的多核苷酸,以及它们的制造和/或使用方法。 含有β-甘露聚糖酶的制剂适用于水解木质纤维素生物质底物,特别是包含可测量水平的半乳糖葡甘露聚糖(GGM)和/或葡甘露聚糖(GM)的那些。

    NOVEL MANNANASE VARIANTS
    5.
    发明公开
    NOVEL MANNANASE VARIANTS 审中-公开
    新的甘露聚糖酶变异

    公开(公告)号:EP2524023A1

    公开(公告)日:2012-11-21

    申请号:EP10788012.2

    申请日:2010-11-24

    申请人: BASF SE

    发明人: KENSCH, Oliver

    摘要: The present disclosure provides novel mannanase variants which have an amino acid sequence that varies from that of the parent/wild type Trichoderma reesei mannanase, and which have one or more advantageous properties like improved thermo stability; temperature/activity profile; pH/activity profile; specific activity; and pH/protease-sensitivity. The novel mannanase variants are useful and used in alcohol fermentations processes and/or productions, for coffee extraction and the processing of coffee waste, as a supplement to food and feed, for enzyme aided bleaching of paper pulps, as bleaching and/or desizing agent in textile industry, for oil and gas well stimulation by hydraulic fracturing, as detergent, as baking ingredients, for removal of biofilms and in delivery systems, for grain processing or for the processing of renewable resources intended for the production of biological fuels, and in the textile, oil drilling, cleaning, laundering, detergent, and cellulose fiber processing industries.

    Endo-Mannanase de café
    9.
    发明公开
    Endo-Mannanase de café 审中-公开
    Endo-Mannanase aus Kaffee

    公开(公告)号:EP1138771A1

    公开(公告)日:2001-10-04

    申请号:EP00201175.7

    申请日:2000-03-30

    摘要: Fragment d'ADN issu de café codant pour au moins une enzyme impliquée dans l'hydrolyse de polysaccharides constitués au moins de molécules de mannanes pures ou ramifiée liées entre elles par un liaison β (1->4), présentant la séquence nucléique SEQ ID NO: 1 ou étant homologue ou s'hybridant à un fragment d'ADN présentant la séquence nucléique SEQ ID NO: 1.

    摘要翻译: 来自咖啡(Coffea arabica)的DNA片段(I)是编码至少一种参与水解多糖(A)的酶,其至少包含通过β(1-4)键连接在一起的直链或支链的甘露聚糖分子。 来自咖啡(Coffea arabica)的DNA片段(I)编码至少一种涉及多糖(A)水解的酶,其至少包含通过β(1-4)键连接在一起的直链或支链的甘露聚糖分子。 (I)是(i)1486bp序列(1),再现; (ii)(1)的同源物,或(iii)能够与(1)的片段杂交。 还包括以下独立权利要求:(A)含有(I)的载体; (B)涉及(A)的水解的咖啡的蛋白质(II),即(i)416个氨基酸序列(2),再现或(ii)与(2)同源的任何序列,其含有三个 特异性肽序列(22,10和17个氨基酸); (C)含有(I)的植物细胞,整合到其基因组中或存在于重组载体上; (D)含有(c)细胞的植物和种子; (E)含有(I)的微生物,整合到基因组中或存在于可复制质粒上,并且能够表达(II); (F)含有(I)或(II)的食品,化妆品或药物组合物; 和(G)使用(II)的全部或部分处理咖啡豆。