摘要:
The present description is related to novel mannanases, compositions comprising mannanase, to methods for producing mannanases and to methods of using mannanases to degrade and modify mannan containing material.
摘要:
The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.
摘要:
The present compositions and methods relate to a beta-mannanase from Thermobifida fusca, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
摘要:
The present disclosure provides novel mannanase variants which have an amino acid sequence that varies from that of the parent/wild type Trichoderma reesei mannanase, and which have one or more advantageous properties like improved thermo stability; temperature/activity profile; pH/activity profile; specific activity; and pH/protease-sensitivity. The novel mannanase variants are useful and used in alcohol fermentations processes and/or productions, for coffee extraction and the processing of coffee waste, as a supplement to food and feed, for enzyme aided bleaching of paper pulps, as bleaching and/or desizing agent in textile industry, for oil and gas well stimulation by hydraulic fracturing, as detergent, as baking ingredients, for removal of biofilms and in delivery systems, for grain processing or for the processing of renewable resources intended for the production of biological fuels, and in the textile, oil drilling, cleaning, laundering, detergent, and cellulose fiber processing industries.
摘要:
A thermostable glycosidase enzymes derived from various thermococcus, staphylothermus and pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.
摘要:
Fragment d'ADN issu de café codant pour au moins une enzyme impliquée dans l'hydrolyse de polysaccharides constitués au moins de molécules de mannanes pures ou ramifiée liées entre elles par un liaison β (1->4), présentant la séquence nucléique SEQ ID NO: 1 ou étant homologue ou s'hybridant à un fragment d'ADN présentant la séquence nucléique SEQ ID NO: 1.
摘要:
The present invention relates to a polypeptide which encodes a mutated mannanase, the sequence of which is derived from a native mannanase of the coprophilic ascomycete filamentous fungus Podospora anserina , and which is characterized by a catalytic efficiency that is increased by at least 25%. The invention also provides a polynucleotide which encodes a polypeptide of the invention, a vector comprising a polynucleotide of the invention, a host cell comprising a vector or a polynucleotide of the invention, and a composition comprising a polypeptide, a polynucleotide, a vector or a host cell of the invention. Finally, the present invention covers the use of a polypeptide, a polynucleotide, a vector, a host cell or a composition of the invention for the degradation of compounds comprising mannans, in particular lignocellulosic biomass, and also the various envisageable uses of a polypeptide, a polynucleotide, a vector, a host cell or a composition of the invention.