摘要:
The present invention relates to new improved peptide or dimeric peptides exhibiting beta-galactosidase enzyme activity wherein the peptide has a pH optimum at acidic conditions.
摘要:
The present invention relates to polypeptides having beta-1,3-galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要:
The present invention relates to an agent for activating mammalian sperm and a method for activating sperm by use of the activating agent. More specifically, the present invention relates to a sperm activating agent containing endo-β-galactosidase and/or FGF, which accelerate motility of mammalian sperm, for use in in-vitro fertilization and artificial insemination by husband and a sperm activating method by adding endo-β-galactosidase and/or FGF.
摘要:
Provided is a method for assaying arylsulfatase activity in aqueous system, which comprises a step for reacting, in an aqueous reaction system with a high ionic strength, arylsulfatase with a substrate thereof, said substrate releasing a fluorophore or a chromophore when subjected to the action of arylsulfatase. Also provided are: a lactase preparation having a lactase activity of 4,000 NLU/g or greater, said lactase activity being assayed by the FCC IV method, and an arylsulfatase activity not greater than 0.1%, said arylsulfatase activity being assayed by the fluorescence method according to the present invention for assaying arylsulfatase activity in aqueous system on the basis of the lactase activity; a method for producing said preparation; and a dairy product with the use of said preparation.
摘要:
The present invention describes an intracellular produced lactase which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.
摘要:
A thermostable glycosidase enzymes derived from various thermococcus, staphylothermus and pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.
摘要:
Methods for providing transgenic plants and parts hereof that, relative to the wild type state, is modified in a complex cell wall polysaccharide structure including pectins and hemicelluloses, the modification being in the overall glycosidic linkage pattern or the monosaccharide profile, comprising transforming a plant cell with a nucleotide sequence that causes an altered production of a complex cell wall polysaccharide-modifying enzyme such as endo-rhamnogalacturonan hydrolase, an endo-rhamnogalacturonan lyase, an endo-galactanase, an endo-arabinanase, an arabinofuranosidase, a galactosidase such as a beta-galactosidase, a xylosidase and an exo-galacturosidase. The modification can occur in vivo or post harvest, in which latter case the modifying enzyme is separated in the growing plant from its substrate, e.g. by targeting the enzyme to the Golgi, the endoplasmic reticulum or a vacuole, or is in a form that is inactive in the plant. After harvest the enzyme is brought into contact with its substrate or it is activated to provide the desired post harvest modification of the cell wall polysaccharide. The transgenic plant materials have improved functionalities and are useful in food and feed manufacturing and as pharmaceutically or medically active substances.
摘要:
The invention describes a method for degrading polysaccharides by using an enzyme preparation comprising endo-β-1,2-galactanase activity. In the process industry and in the paper machine environment in particular, microbes form biofilms on surfaces, consisting of microbes, fibres, and polysaccharide that protects the microbe cells, increases the viscosity of the biofilm, and protects the biofilm against drying. By treating biofilms and other slime deposits in the process industry with the enzyme preparation according to the invention, the problems caused by biofilms to the process industry can be reduced. Endo-β-1,2-galactanase can also be used in the preparation of various mono- or oligosaccharides.