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    BREWING PROCESS
    6.
    发明公开
    BREWING PROCESS 审中-公开
    酿造工艺

    公开(公告)号：EP2346978A2

    公开(公告)日：2011-07-27

    申请号：EP09783851.0

    申请日：2009-10-08

    申请人： Novozymes A/S

    发明人： KREISZ, Stefan ,  FREDERIKSEN, Anne Mette

    IPC分类号： C12C5/00 ,  C12C7/04

    CPC分类号： C12C5/004 ,  C12C7/04 ,  C12Y302/01041 ,  C12Y302/01068

    摘要： A process for producing a wort comprising the steps of mixing a grist with water, adding a debranching enzyme, wherein said debranching enzyme has above 60% enzyme activity, at 64°C, for a period of 10 minutes, at pH 5.0, resting said mixture at 58-68°C for a period of 10-40 minutes, resting said mixture at 72-80°C, for a period of 5-20 minutes, and separating the wort from solid components.

    METHOD FOR DESIGNING MUTATED ENZYME, METHOD FOR PREPARING THE SAME AND MUTATED ENZYME
    8.
    发明公开
    METHOD FOR DESIGNING MUTATED ENZYME, METHOD FOR PREPARING THE SAME AND MUTATED ENZYME 有权
    法用于生产建筑突变酶，方法以及突变体酶

    公开(公告)号：EP2058394A1

    公开(公告)日：2009-05-13

    申请号：EP07767790.4

    申请日：2007-06-28

    申请人： Amano Enzyme Inc.

    发明人： MIKAMI, Bunzo ,  IWAMOTO, Hiroyuki ,  YAMAGUCHI, Shotaro

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/44

    CPC分类号： C12Y302/01041 ,  C07K2299/00 ,  C12N9/2451 ,  C12N9/2457 ,  C12N9/246 ,  C12Y302/01068

    摘要： It is intended to provide a novel method for improving an enzyme hydrolyzing an a-1,6-glycosidic linkage. A mutated enzyme is designed by specifying one or more amino acids selected from the group shown below in an amino acid sequence of an enzyme (an enzyme to be mutated) that hydrolyzes an a-1,6-glycosidic linkage, that is, the group consisting of an amino acid corresponding to an amino acid at the 292 position, an amino acid corresponding to an amino acid at the 371 position, an amino acid corresponding to an amino acid at the 406 position, an amino acid corresponding to an amino acid at the 407 position, an amino acid corresponding to an amino acid at the 437 position, an amino acid corresponding to an amino acid at the 465 position, an amino acid corresponding to an amino acid at the 475 position, an amino acid corresponding to an amino acid at the 476 position,; an amino acid corresponding to an amino acid at the 525 position, an amino acid corresponding to an amino acid at the 526 position, an amino acid corresponding to an amino acid at the 580 position and an amino acid corresponding to an amino acid at the 582 position of the amino acid represented by in SEQ ID NO: 2 (step (1)) and constructing an amino acid sequence in which the amino acid(s) specified in the step (1) is/are substituted with another amino acid or deleted based on the amino acid sequence of the enzyme to be mutated (step (2)).

    摘要翻译： 它的目的是用于酶的改善水解α-1,6糖苷键提供一种新颖的方法。 突变的酶是通过指定从在酶（待突变的酶）的氨基酸序列在下面所示的基团中选择的一个或多个氨基酸而设计确实水解成α-1,6糖苷键，所做的是，该组 在氨基酸组成的氨基酸在氨基酸在292位对应于，在氨基酸在氨基酸371位对应于，在氨基酸在氨基酸在位置406对应于在氨基酸对应于 在407位，在氨基酸在氨基酸在437位对应于，在氨基酸在氨基酸在465位对应于，在氨基酸在氨基酸475位对应于，在氨基酸对应于氨基 酸在476位置，; 在氨基酸对应于在525位氨基酸，在氨基酸处的526位氨基酸对应于，在氨基酸在氨基酸在580位和氨基酸在582处的氨基酸与对应于 由SEQ ID NO所代表的氨基酸的位置：2（步骤（1）），并且其中所述的氨基酸（S）在所述步骤中指定的氨基酸序列的构建（1）/是substituiertem成其他氨基酸或删除 基于酶的氨基酸序列中突变的（步骤（2））。