公开(公告)号：EP2941481A4

公开(公告)日：2016-11-02

申请号：EP14735413

申请日：2014-01-06

申请人： NOVOGY INC

发明人： SOUTH COLIN R ,  SHAW ARTHUR J IV

IPC分类号： C12N1/00 ,  C12N15/74

CPC分类号： C12N15/815 ,  C12N1/20 ,  C12N9/14 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12P3/00 ,  C12Y308/01 ,  C12Y402/01069

摘要： Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.

摘要翻译： 公开了具有代谢非典型氮源（例如三聚氰胺和氨腈）的能力的遗传工程生物，例如酵母和细菌。 还描述了使用遗传工程生物的发酵方法。 本发明的方法是用于各种化合物（包括商品，精细化学品和药物）的工业生物生产的可靠工艺。

公开(公告)号：EP2941481A2

公开(公告)日：2015-11-11

申请号：EP14735413.8

申请日：2014-01-06

申请人： Novogy Inc.

发明人： SOUTH, Colin, R. ,  SHAW, Arthur, J., IV

IPC分类号： C12N1/00 ,  C12N15/74

CPC分类号： C12N15/815 ,  C12N1/20 ,  C12N9/14 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12P3/00 ,  C12Y308/01 ,  C12Y402/01069

摘要： Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.

公开(公告)号：EP2502990A3

公开(公告)日：2013-04-17

申请号：EP12153531.4

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzins, Aldis ,  Zhao, Kate Qin ,  Encell, Lance P. ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V. ,  Wood, Monika G. ,  Zimmerman, Kris ,  Slater, Michael R.

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

公开(公告)号：EP2374875A2

公开(公告)日：2011-10-12

申请号：EP11075056.9

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzin, Aldis ,  Encell, Lance P ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V ,  Wood, Monika G ,  Zimmerman, Kris ,  Slater, Michael R ,  Zhao, Kate Qin

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

公开(公告)号：EP2087107A2

公开(公告)日：2009-08-12

申请号：EP07867352.2

申请日：2007-10-30

申请人： PROMEGA CORPORATION

发明人： DARZINS, Aldis ,  ENCELL, Lance, P. ,  OHANA, Rachel, Friedman ,  OTTO, Paul ,  VIDUGIRIS, Gediminas ,  WOOD, Keith, V. ,  WOOD, Monika, G. ,  ZIMMERMAN, Kris ,  SLATER, Michael, R. ,  ZHAO, Kate, Qin

IPC分类号： C12N9/14

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

摘要翻译： 本发明提供了具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。

公开(公告)号：EP2502990B1

公开(公告)日：2015-07-22

申请号：EP12153531.4

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzins, Aldis ,  Zhao, Kate Qin ,  Encell, Lance P. ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V. ,  Wood, Monika G. ,  Zimmerman, Kris ,  Slater, Michael R.

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

公开(公告)号：EP2492342B1

公开(公告)日：2015-06-03

申请号：EP12153529.8

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzins, Aldis ,  Zhao, Kate Qin ,  Encell, Lance P. ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V. ,  Wood, Monika G. ,  Zimmerman, Kris ,  Slater, Michael R.

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

公开(公告)号：EP2502990A2

公开(公告)日：2012-09-26

申请号：EP12153531.4

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzins, Aldis ,  Zhao, Kate Qin ,  Encell, Lance P. ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V. ,  Wood, Monika G. ,  Zimmerman, Kris ,  Slater, Michael R.

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

摘要翻译： 本发明提供了具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸和使用该多核苷酸和突变蛋白的方法。

公开(公告)号：EP2374875B1

公开(公告)日：2015-09-02

申请号：EP11075056.9

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzin, Aldis ,  Encell, Lance P ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V ,  Wood, Monika G ,  Zimmerman, Kris ,  Slater, Michael R ,  Zhao, Kate Qin

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

摘要翻译： 本发明提供了具有增强的动力学和功能表达的突变水解酶蛋白质，以及编码突变蛋白质的多核苷酸以及使用多核苷酸和突变蛋白质的方法。

公开(公告)号：EP2374875A3

公开(公告)日：2012-10-10

申请号：EP11075056.9

申请日：2007-10-30

申请人： Promega Corporation

发明人： Darzin, Aldis ,  Encell, Lance P ,  Ohana, Rachel Friedman ,  Otto, Paul ,  Vidugiris, Gediminas ,  Wood, Keith V ,  Wood, Monika G ,  Zimmerman, Kris ,  Slater, Michael R ,  Zhao, Kate Qin

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.