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公开(公告)号：EP0173276A3
公开(公告)日：1988-08-24
申请号：EP85110679
申请日：1985-08-26
摘要： 57 The determination of a substrate or an activity of an enzyme in a sample containing ammonia by using a reaction system which forms ammonia can be performed by decomposing ammonia in the sample with an ammonia-decomposing reagent system, adding a reagent system necessary for the formation of ammonia from the substrate or the substrate for the enzyme to form ammonia and determining the formed ammonia.
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公开(公告)号：EP0079792A3
公开(公告)日：1983-11-09
申请号：EP82306073
申请日：1982-11-15
发明人： Tamura, Shigeaki , Endo, Kenichi , Katsumata, Hideo , Arai, Yuko
摘要： Urease having 1 ~ 5 x 10 2 Km value is produced by culturing a microorganism of the genus Corynebactenum, Breivibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella. The enzyme is useful for the determination of urea in the rate assay method.
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公开(公告)号：EP0207493A3
公开(公告)日：1987-09-02
申请号：EP86108933
申请日：1986-07-01
摘要： Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of a chelating agent in a system wherein NADP + formed from NADPH is reproduced into NADPH in the conjoint presence of an isocitrate, metallic ions such as magnesium or manganese ions, and isocitrate dehydrogenase in assaying a substance by means of a reaction of NADPH to NADP + .
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4.发明公开
公开(公告)号：EP0204438A3
公开(公告)日：1987-05-27
申请号：EP86303493
申请日：1986-05-08
CPC分类号： C12Q1/04 , C12Q1/58 , G01N2333/205 , Y02A50/451
摘要： The invention relates to a composition for the detection of preformed urease to provide a diagnosis of gastrointestinal disorders which comprises
(a) urea; (b) a bactericide; (c) a pH indicator which undergoes a colour change upon an increase of pH, at an effective concentration; and (d) water; wherein said composition has an acid pH of at least 5.0 and the pH of said composition is at least about one pH unit lower than the pK a of said indicator.-
公开(公告)号：EP0164119A3
公开(公告)日：1988-09-14
申请号：EP85107001
申请日：1985-06-05
摘要： Bei der kolorimetrischen Harnstoffbestimmung in Milch wird die Eignung der Milchprobe durch Ermittlung des Blind wertes nach Abbau des Harnstoffs mittels Urease festgestellt. Bei Blindwerten über 5 mg/100 ml ist die Milchprobe unge eignet.
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6.发明公开Method for quantitative analysis of analyte in liquid sample using analytical element 失效使用分析元素的液体样品中分析物的定量分析方法
公开(公告)号：EP0212660A3
公开(公告)日：1988-01-13
申请号：EP86111800
申请日：1986-08-26
发明人： Iwata, Yuzo c/o Fuji Photo Film Co., Ltd. , Makiuchi, Hajima c/o Fuji Photo Film Co., Ltd. , Kitajima, Masao c/o Fuji Photo Film Co., Ltd.
CPC分类号： C12Q1/58 , C12Q1/54 , G01N21/274 , G01N21/4738 , G01N21/8483 , G01N33/525
摘要： In a method for quantitatively analyzing an analyte in a liquid sample by applying the liquid sample on one of a set of analytical elements and measuring a color developed in the analytical element through reflection photometory in which said set of analytical elements are the same elements as a standard element except for deviation of sensitivity to the analyte, but said deviation of sensitivity is essentially equivalent to each other among the set of analytical elements, the calibration curve of the standard element with respect to said analyte being predetermined, an improvement involving preparation of a linear equation for defining a relationship between an optical reflection density value OD x to be measured on an analytical element belonging to said set of the analytical elements and an optical reflection density value OD s being predetermined on the standard analytical element, in which the optical reflection density values OD x and OD s are those to be determined on colors developed on the respective elements upon application of a liquid sample containing the analyte in the same content C x .
摘要翻译： 在通过将液体样品应用于一组分析元件之一并通过反射光度测量在分析元件中显影的颜色来定量分析液体样品中的分析物的方法中，其中所述一组分析元件是与 除了对分析物的敏感性偏差之外，所述灵敏度的偏差在所述一组分析元件中基本相同，所述标准元件相对于所述分析物的校准曲线是预定的，改进涉及制备 用于定义属于所述分析单元组的分析单元的光反射浓度值ODx与标准分析单元上规定的光反射浓度值OD之间的关系的线性方程式，其中光学反射浓度 值ODx和OD是在颜色设计上确定的值 在将含有相同含量C x的分析物的液体样品应用于相应的元件上。
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公开(公告)号：EP0069488A3
公开(公告)日：1983-03-30
申请号：EP82303157
申请日：1982-06-17
IPC分类号： C12Q01/58
CPC分类号： C12N9/0016 , C12N9/80 , C12Q1/58
摘要： A urea assay mixture, particularly for use in the determination of urea in physiological fluids such as blood, which comprises urease and glutamate dehydrogenase enzymes from the same micro-organism source together with cofactors. The application also discloses a process for the production of the mixture, a method for the purification together of the enzymes urease and glutamate dehydrogenase and a method for testing for urea in a fluid using the assay mixture.
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公开(公告)号：EP0251689A3
公开(公告)日：1988-06-01
申请号：EP87305639
申请日：1987-06-24
申请人： ALLELIX INC.
IPC分类号： G01N33/543 , G01N33/76 , C12Q01/58
CPC分类号： G01N33/5302 , G01N33/54366 , Y10S436/807 , Y10S436/81
摘要： A device for performing a plurality of immunochemical assays simultaneously comprises a waste reservoir (11) about which are positioned at least two assay tubes (16) connected in series by means of conduits (32) (38) formed in the base (12,30) and top (25) of the reservoir structure. A compound syringe (20) is insertable into an opening (29) formed through the top of the waste reservoir, the syringe being held in place by a receiving structure (47) formed in the base of the reservoir and connected to the entrance end of the series of assay tubes. The various compartments of the compound syringe are emptied in sequence to cause the flow of sample and assay reagents to flow through the assay tubes. The device is structured to allow for the reflow of sample through the tubes if desired.
摘要翻译： 用于执行多个免疫化学测定的装置同时包括废物储存器（11），废物储存器（11）通过形成在基座（12,30）中的管道（32）（38）定位在至少两个测定管（16） ）和储层结构的顶部（25）。 复合注射器（20）可插入通过废物储存器的顶部形成的开口（29）中，注射器通过形成在储存器的底部中的接收结构（47）保持就位，并且连接到 一系列测定管。 复合注射器的各个隔室依次排空，导致样品和测定试剂的流动流过测定管。 如果需要，该装置被构造成允许样品通过管的回流。
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公开(公告)号：EP0135092A3
公开(公告)日：1987-04-22
申请号：EP84109133
申请日：1984-08-01
摘要： The quantity of ammonia in a sample to be measured for ammonia content can be determined by:
(A) contacting the sample with L-glutamate dehydrogenase in the presence of reduced nicotinamide-adenine dinucleotide or reduced nicotinamide-adenine dinucleotide phosphate and a-ketoglutarate to produce glutamate in a quantity corresponding to the ammonia content in the sample; (B) catalyzing the resultant mixture by L-glutamate oxidase in the presence of oxygen to produce hydrogen peroxide in a quantity corresponding to the glutamate content in the resultant mixture; (C) measuring the quantity of hydrogen peroxide produced or the production rate thereof; and (D) calculating the ammonia content from the quantity of hydrogen peroxide produced or the production rate thereof thus measured. In accordance with the method of the present invention described above, it has become possible to determine the quantity of ammonia with high sensitivity in a short period of time by using as a signal a measurable value associated with hydrogen peroxide via a continuous autoanalyzing system.-
公开(公告)号：EP0133681A3
公开(公告)日：1986-03-12
申请号：EP84108995
申请日：1984-07-30
申请人： Miles Italiana SpA
发明人： Fossati, Piero
IPC分类号： C12Q01/58
CPC分类号： C12Q1/58
摘要： A composition, device and method useful as an enzymatic urea assay based on the use of urea amidolyase comprising urea amidolyase, pyruvate kinase, pyruvate oxidase, mono and divalent cations, phosphoenol pyruvate, thyamine pyrophosphate, ATP, bicarbonate, phosphate, a color indicator system and optionally inorganic phosphate, a buffer having a pH range of from about 6.5 to 9.5, and sodium or potassium ferrocyanide. After contacting the test solution or body fluid sample with the assay composition, reading can be accomplished visually or instrumentally.
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