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    VECTOR AND METHOD FOR SCREENING FUNCTIONAL ANTIGEN-BINDING PROTEIN
    2.
    发明公开
    VECTOR AND METHOD FOR SCREENING FUNCTIONAL ANTIGEN-BINDING PROTEIN 审中-公开

    公开(公告)号：EP4317549A1

    公开(公告)日：2024-02-07

    申请号：EP22779050.8

    申请日：2022-03-30

    申请人： Ddbio. Co, Ltd., (Shang Hai)

    发明人： ZHOU, Chen

    IPC分类号： C40B50/06 ,  C40B40/02

    摘要： A method for selecting a functional antigen-binding protein. The method comprises the step of constructing an expression vector, by means of a phage display technique and/or a cell display technique, using a light chain or a fragment thereof of a reference antigen-binding protein with a known sequence and function, or a heavy chain or a fragment thereof of the reference antigen-binding protein, thereby selecting a functional antigen-binding protein that binds to the same epitope as the reference antigen-binding protein. Further provided is a functional antigen-binding protein obtained by means of the method.

    METHOD FOR RAPIDLY CONSTRUCTING CYCLIZED LIBRARY AND RING-FORMING LINKER
    5.
    发明公开
    METHOD FOR RAPIDLY CONSTRUCTING CYCLIZED LIBRARY AND RING-FORMING LINKER 审中-公开

    公开(公告)号：EP4273307A1

    公开(公告)日：2023-11-08

    申请号：EP20967409.2

    申请日：2020-12-29

    申请人： MGI Tech Co., Ltd.

    发明人： YU, Youqian ,  FU, Shujin ,  GENG, Chunyu ,  LIANG, Xinming

    IPC分类号： C40B40/06 ,  C40B50/06 ,  C40B80/00

    摘要： The present invention falls within the field of biotechnology, and specifically discloses a method for constructing a cyclized library and a ring-forming linker. The method comprises: 1) breaking a DNA sequence into fragments; 2) enabling two ends of the broken fragment to form 3' end protrusions; and 3) cyclizing the fragment with 3' end protrusions to form a ring-shaped library by means of a ring-forming linker, wherein the ring-forming linker is a double chain which is not completely paired and has 3' end protrusion at both ends, and the 3' end protrusion of the ring-forming linker is complementary to the 3' end protrusion of the broken fragment. According to the present invention, an A-sticky end of the end is formed by means of repairing the end of and the adding A to the broken DNA fragment, and same is then complementary to the specifically designed linker T sticky end to form a cyclized structure, and the connection at the gap is completed under the action of a ligase.

    IN-VITRO CULTURE, INDUCTION, ACTIVATION AND CRYOPRESERVATION METHOD AND CELL BANK ESTABLISHMENT FOR IMMUNE CELLS
    7.
    发明公开
    IN-VITRO CULTURE, INDUCTION, ACTIVATION AND CRYOPRESERVATION METHOD AND CELL BANK ESTABLISHMENT FOR IMMUNE CELLS 审中-公开

    公开(公告)号：EP4148123A1

    公开(公告)日：2023-03-15

    申请号：EP20934683.2

    申请日：2020-05-26

    申请人： Qingdao Restore Biotechnology Co., Ltd.

    发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng

    IPC分类号： C12N5/0783 ,  C40B50/06 ,  A01N1/02

    摘要： The present invention discloses an in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells. The method includes the follows: using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells; using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells; using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions; using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells; and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file of immune cells for retrieval to construct an immune cell bank.

    METHOD FOR BUILDING RNA LIBRARY AND KIT
    9.
    发明公开
    METHOD FOR BUILDING RNA LIBRARY AND KIT 审中-公开

    公开(公告)号：EP3848473A1

    公开(公告)日：2021-07-14

    申请号：EP18932607.7

    申请日：2018-09-05

    申请人： BGI Shenzhen

    发明人： YANG, Xi ,  WANG, Wenjing ,  XING, Yanru ,  XU, Jinjin ,  CHEN, Fang ,  ZHU, Shida

    IPC分类号： C12Q1/68 ,  C40B50/06

    摘要： Provided are an RNA library construction method and a specialized kit thereof. The method for preparing an RNA library includes the following steps: extracting RNA and performing fragmentation; adding a tail to 3' end; ligating an adaptor to 5' end and hybridizing with a DNA probe mixture; the DNA probe mixture is composed of several DNA probes that are reverse complementary to an RNA that is expected to be removed; removing RNA from the hybrid and removing DNA; performing reverse transcription and PCR amplification to obtain a library solution. The present invention combines poly A tailing and 5'end ligation. The RNA content in the system can be quickly increased by polyA tailing, thereby avoiding subsequent purification losses.