3-dimensional confocal observation apparatus and observation focal plane displacement and correction unit

    公开(公告)号:JP5221614B2

    公开(公告)日:2013-06-26

    申请号:JP2010208971

    申请日:2010-09-17

    Abstract: Provided is a 3-dimensional confocal microscopy apparatus which is manufactured by combining a confocal microscope and an optical tweezers technique, wherein a pair of lenses for focal plane displacement where one lens is movable in the optical axis direction is arranged between a fixed objective lens and a fluorescent light imaging camera, and the 3-dimensional confocal microscopy apparatus also includes a mean which corrects the aberration of a fluorescent confocal image obtained by the fluorescent imaging camera. Accordingly, it is possible to provide a 3-dimensional confocal microscopy apparatus which can acquire a 3-dimensional image of a specimen during a manipulation of the specimen using optical tweezers without affecting an optical trap.

    Abstract translation: 提供一种通过组合共聚焦显微镜和光学镊子技术制造的三维共焦显微镜装置,其中一对透镜在光轴方向上可移动的用于焦平面位移的透镜被布置在固定物镜和 荧光成像照相机和三维共聚焦显微镜装置还包括校正由荧光成像相机获得的荧光共焦图像的像差的平均值。 因此,可以提供一种三维共聚焦显微镜装置,其可以在不影响光阱的情况下使用光学镊子在使用镊子的操作期间获取样本的3维图像。

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