公开(公告)号：JP2014140341A

公开(公告)日：2014-08-07

申请号：JP2013012013

申请日：2013-01-25

申请人： Bonac Corp ,  株式会社ボナック

发明人： HAMAZAKI TOMOHIRO ,  MIZUTANI TAKAYUKI ,  KATO SHIORI ,  OKI TADAAKI

IPC分类号： C12N15/09

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule that can inhibit expression of RPN2 gene in treatment of drug-resistant breast cancer.SOLUTION: The nucleic acid molecule for inhibiting the expression of RPN2 gene comprises any one of the nucleotide sequences of the following (as1), (as2) and (as3) as the sequence to inhibit the expression of RPN2 gene, where (as1) is nucleotides of a specified base sequence; (as2) is nucleotides having deletion, substitution and/or addition of one or several bases in the base sequence of (as1) and having a function inhibiting the expression of RPN2 gene being a target of drug-resistant breast cancer treatment; and (as3) is nucleotides having 90% or more homology to the base sequence of (as1).

摘要翻译： 要解决的问题：提供能够抑制RPN2基因在耐药性乳腺癌治疗中的表达的新型核酸分子。解决方案：用于抑制RPN2基因表达的核酸分子包含任何一个核苷酸序列 （as1），（as2）和（as3）作为抑制RPN2基因表达的序列，其中（as1）是指定碱基序列的核苷酸; （as2）是具有在（as1）的碱基序列中缺失，取代和/或添加一个或几个碱基并具有抑制作为耐药性乳腺癌治疗靶标的RPN2基因表达的功能的核苷酸; 和（as3）是与（as1）的碱基序列具有90％或更高同源性的核苷酸。

公开(公告)号：JP2013138681A

公开(公告)日：2013-07-18

申请号：JP2013048777

申请日：2013-03-12

申请人： Bonac Corp ,  株式会社ボナック

发明人： OKI TADAAKI ,  HAYASHI HIROTAKE ,  SHIRAMIZU HISAO ,  HAMAZAKI TOMOHIRO ,  ITO AKIHIRO ,  SUZUKI HIROSHI

IPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K48/00 ,  A61P29/00 ,  C12N15/09

CPC分类号： C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/51 ,  C12N2330/30

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule that can be produced by an easy and efficient production method and can inhibit expression of a gene.SOLUTION: There is provided a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene, wherein, the molecule includes a region (X), a linker region (Lx), and a region (Xc). The linker region (Lx) is linked between the regions (X) and (Xc), the region (Xc) is complementary to the region (X), at least one of the regions (X) and (Xc) includes the expression inhibitory sequence, and the linker region (Lx) has a non-nucleotide structure including at least one of a pyrrolidine skeleton and a piperidine skeleton. According to this single-stranded nucleic acid molecule, it is possible to inhibit the expression of the target gene.

摘要翻译： 要解决的问题：提供可以通过简单且有效的制备方法制备并可以抑制基因表达的新型核酸分子。解决方案：提供包含抑制基因表达的表达抑制性序列的单链核酸分子 目标基因的表达，其中，分子包括区域（X），接头区域（Lx）和区域（Xc）。 接头区域（Lx）连接在区域（X）和（Xc）之间，区域（Xc）与区域（X）互补，区域（X）和（Xc）中的至少一个包括表达抑制 序列，并且接头区域（Lx）具有包括吡咯烷骨架和哌啶骨架中的至少一种的非核苷酸结构。 根据该单链核酸分子，可以抑制靶基因的表达。

公开(公告)号：JP2012130342A

公开(公告)日：2012-07-12

申请号：JP2012009515

申请日：2012-01-20

申请人： Bonac Corp ,  株式会社ボナック

发明人： OKI TADAAKI ,  HAYASHI HIROTAKE ,  SHIRAMIZU HISAO ,  HAMAZAKI TOMOHIRO ,  ITO AKIHIRO ,  SUZUKI HIROSHI

IPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K48/00 ,  A61P29/00

CPC分类号： C12N15/111 ,  C12N2310/14 ,  C12N2310/318 ,  C12N2310/53 ,  C12N2310/531 ,  C12N2310/533

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule which can control gene expression and can be easily and efficiently produced.SOLUTION: There is disclosed a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits the expression of a target gene. The single-stranded nucleic acid molecule includes a 5' side region (Xc), an inner region (Z), and a 3' side region (Yc) in the order from the 5' side to the 3' side. The inner region (Z) is composed of an inner 5' side region (X) and an inner 3' side region (Y) that are linked to each other. The 5' side region (Xc) is complementary to the inner 5' side region (X). The 3' side region (Yc) is complementary to the inner 3' side region (Y). At least one of the inner region (Z), the 5' side region (Xc), and the 3' side region (Yc) includes the expression inhibitory sequence. According to this single-stranded nucleic acid molecule, it is possible to inhibit the expression of the target gene.

摘要翻译： 要解决的问题：提供可以控制基因表达并且可以容易且有效地产生的新的核酸分子。 解决方案：公开了包含抑制靶基因表达的表达抑制性序列的单链核酸分子。 单链核酸分子以从5'侧到3'侧的顺序包括5'侧区域（Xc），内部区域（Z）和3'侧面区域（Yc）。 内部区域（Z）由彼此连接的内部5'侧区域（X）和内部3'侧区域（Y）构成。 5'侧区域（Xc）与内部5'侧区域（X）互补。 3'侧区域（Yc）与内3'侧区域（Y）互补。 内部区域（Z），5'侧区域（Xc）和3'侧区域（Yc）中的至少一个包括表达抑制序列。 根据该单链核酸分子，可以抑制靶基因的表达。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2013153736A

公开(公告)日：2013-08-15

申请号：JP2012026746

申请日：2012-02-09

申请人： Bonac Corp ,  株式会社ボナック

发明人： OKI TADAAKI ,  SUZUKI HIROSHI ,  HAMAZAKI TOMOHIRO

IPC分类号： C12N15/113 ,  A61K31/7115 ,  A61K48/00 ,  A61P11/00 ,  A61P29/00 ,  A61P43/00

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule allowing easy production method thereof with good efficiency, and inhibiting the expression of a gene.SOLUTION: This single strand nucleic molecule including an expression inhibitory sequence inhibiting the expression of a target gene, includes a region (X), a linker region (Lx) and a region (Xc). The linker region (Lx) is connected between the region (Xc) and region (Xc). The region (Xc) is complemental to the region (X). At least one of the region (X) and region (Xc) includes the expression inhibitory sequence, and the linker region (Lx) includes a peptide structure. By the single strand nucleic molecule, the expression of the target gene can be inhibited.

摘要翻译： 要解决的问题：提供一种新的核酸分子，其能够容易地制备其制备方法，并且抑制基因的表达。解决方案：包括抑制靶基因表达的表达抑制性序列的单链核酸分子包括 区域（X），接头区域（Lx）和区域（Xc）。 连接区域（Lx）连接在区域（Xc）和区域（Xc）之间。 区域（Xc）与区域（X）互补。 区域（X）和区域（Xc）中的至少一个包括表达抑制性序列，并且接头区域（Lx）包括肽结构。 通过单链核酸分子，可以抑制靶基因的表达。

公开(公告)号：JP2013055913A

公开(公告)日：2013-03-28

申请号：JP2011196742

申请日：2011-09-09

申请人： Bonac Corp ,  株式会社ボナック

发明人： OKI TADAAKI ,  HAYASHI HIROTAKE ,  HAMAZAKI TOMOHIRO ,  SHIRAMIZU HISAO

IPC分类号： C12N15/113

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule suppressible of gene expression.SOLUTION: This single-stranded RNA molecule includes an expression-suppressing sequence suppressing expression of a target gene, including, from 5' side to 3' side, a 5' side region (Xc), a linker region (Lx) and a 3' side region (X) in this order; where the 5' side region (Xc) is complementary to the 3' side region (X), at least one of the 5' side region (Xc) and the 3' side region (X) contains the expression-suppressing sequence, and the molecule satisfies at least either condition of the following (1) and (2). The condition (1): a 3' terminal region of the 5' side region (Xc) and a 5' terminal region of the 3' side region (X) hybridize by a hydrogen bonding between an adenine base and a urasil base. The condition (2): at least either of the 3' terminal region of the 5' side region (Xc) and the 5' terminal region of the 3' side region (X) has a deoxyribonucleotide residue.

摘要翻译： 要解决的问题：提供可抑制基因表达的新型核酸分子。 解决方案：该单链RNA分子包括抑制靶基因表达的表达抑制序列，包括从5'侧至3'侧的5'侧区（Xc），接头区（Lx） 和3'侧区域（X）; 其中5'侧区域（Xc）与3'侧区域（X）互补，5'侧区域（Xc）和3'侧区域（X）中的至少一个包含表达抑制序列，并且 分子满足以下（1）和（2）的至少任一条件。 条件（1）：5'侧区域（Xc）的3'末端区域和3'侧区域（X）的5'末端区域通过腺嘌呤碱基和尿嘧啶碱基之间的氢键杂交。 条件（2）：5'侧区域（Xc）的3'末端区域和3'侧区域（X）的5'末端区域中的至少任一个具有脱氧核糖核苷酸残基。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2013034470A

公开(公告)日：2013-02-21

申请号：JP2012026878

申请日：2012-02-10

申请人： BONAC CORP

发明人： OKI TADAAKI ,  HAYASHI HIROTAKE ,  SHIRAMIZU HISAO ,  HAMAZAKI TOMOHIRO ,  ITO AKIHIRO ,  SUZUKI HIROSHI

IPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K47/22 ,  A61K47/34 ,  A61K47/48 ,  A61K48/00 ,  A61P29/00 ,  A61P43/00 ,  C07D207/08

摘要： PROBLEM TO BE SOLVED: To provide a new nucleic acid molecule that can be produced easily and efficiently, and can inhibit the expression of a gene.SOLUTION: This single-stranded nucleic acid molecule includes an expression inhibitory sequence that inhibits the expression of a target gene, containing a region (X); a linker region (Lx); and a region (Xc). The linker region (Lx) is linked between the region (X) and region (Xc), and the region (Xc) is complementary to the region (X). At least one of the region (X) and region (Xc) contains the expression inhibitory sequence, and the linker region (Lx) has a non-nucleotide structure containing at least one of a pyrrolidine skeleton and a piperidine skeleton. The expression of the target gene can be inhibited according to the single-stranded nucleic acid molecule.