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公开(公告)号：JP2009156722A
公开(公告)日：2009-07-16
申请号：JP2007335523
申请日：2007-12-27
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： YOKOSUKA TOSHIYUKI , YOSHINARI KIYOMI , KOBAYASHI KINYA
IPC: G01N27/62
Abstract: PROBLEM TO BE SOLVED: To overcome the problem wherein mapping of components is difficult by a shortage of a separation due to mixture of a plurality of the components, fluctuations in the intensity between measurements, fluctuations in the retention times in chromatography or fluctuations in samples due to pretreatments, even if measurements are implemented under conditions similar to chromatography mass spectrometry of biological samples.
SOLUTION: Ion information (masses, valences, intensities, isotope peak distribution, retention times) on a MS spectrum of the components to be mapped and MS spectrum information on the other components detected in the mass and time zone similar to the components to be mapped are utilized so as to implement mapping of the components among a plurality of the chromatography mass spectrometric data. Information on the other component includes the masses, the valences, the intensities, the isotope peak distribution, the retention times, and the like. The degree of matching is estimated as a score. The mapping accuracy is improved, by utilizing the component information detected in a similar time zone, in addition to the components to be mapped during determination.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题：为了克服由于多个组分的混合而导致的分离不足导致组分的映射困难的问题，测量之间的强度的波动，色谱中的保留时间的波动或波动 在样品中由于预处理，即使在类似于生物样品的色谱质谱的条件下进行测量。 解决方案：要映射的组件的MS频谱上的离子信息（质量，价态，强度，同位素峰值分布，保留时间）和质谱和时间带中检测到的其他成分的质谱信息类似于组分 被使用以便实现多个色谱质谱数据之间的组分的映射。 关于另一部分的信息包括质量，价态，强度，同位素峰分布，保留时间等。 匹配度被估计为一个分数。 除了在确定期间要映射的组件之外，通过利用在相似时区中检测到的分量信息来提高映射精度。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2006329881A
公开(公告)日：2006-12-07
申请号：JP2005156157
申请日：2005-05-27
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： OTAKE ATSUSHI , KOBAYASHI KINYA , YOKOSUKA TOSHIYUKI , YOSHINARI KIYOMI
CPC classification number: H01J49/02 , H01J49/004
Abstract: PROBLEM TO BE SOLVED: To automatically determine and process peptides derived from a very small amount of proteins as targets of tandem mass spectrometry desired by users without any measurement waste.
SOLUTION: In this tandem type analysis system, by ionizing an object to be measured and performing mass spectrometry on generated various ion species, ion species having a specific mass-to-charge ratio are selected from among the generated various ion species and dissociated, and ion mass spectrometry measurement is repeated at n-stages (n=1, 2, etc.). On the basis of ionic strength expressed by a peak to the mass-to-charge ratio of ions in results of MS
n , mass spectrometry at the n-th stage, control contents of the next analysis of MS
n are determined for every ion to be analyzed. An ionization detector 14 highly precisely collates sample measurement and ionization data and determines the peaks of isotopes. When a count number of MS
1 of a parent ion peptide measured for some prescribed period is, for example, I, a data processing part 15 makes the number of times of integration of MS
2 of the peptide or its analysis time proportional to 1/I.
COPYRIGHT: (C)2007,JPO&INPITAbstract translation: 要解决的问题：自动确定和处理由非常少量的蛋白质衍生的肽作为用户所期望的串联质谱的目标，而没有任何测量浪费。 解决方案：在这种串联型分析系统中，通过电离待测物体并对生成的各种离子种类进行质谱分析，从生成的各种离子种类中选出具有特定质荷比的离子种类， 离解和离子质谱测量在n阶段重复（n = 1,2等）。 基于离子强度表示的离子强度与离子的质荷比在MS
n 的结果中，第n阶段的质谱法，下一步MS分析的控制内容 对于要分析的每个离子确定 n 。 电离检测器14高度精确地对照样品测量和电离数据并确定同位素的峰。 当在某个规定时间内测量的母离子肽的MS 1 的计数是例如I时，数据处理部15使MS 2的积分次数< / SP>或其分析时间与1 / I成比例。 版权所有（C）2007，JPO＆INPIT -
公开(公告)号：JP2004257922A
公开(公告)日：2004-09-16
申请号：JP2003050485
申请日：2003-02-27
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： OTAKE ATSUSHI , KOBAYASHI KINYA , YOSHINARI KIYOMI , HIRABAYASHI TSUDOI , WAKE IZUMI
CPC classification number: H01J49/004 , H01J49/0036 , Y10T436/24
Abstract: PROBLEM TO BE SOLVED: To enhance measurement throughput and specimen identification accuracy in a tandem mass spectrometry. SOLUTION: In this analysis system for a mass spectrometry spectrum using the tandem mass spectrometry for repeating, by n-steps, selection, dissociation, and measurement for an ionic species serving as a measuring object, an ionic species to be measured at MS n is selected based on a mass-to-charge ratio (m/z value) as a spectrum analysis result of MS n-1 (n≥2), and this is repeated until a required number of amino-acid sequences are determined. COPYRIGHT: (C)2004,JPO&NCIPI
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公开(公告)号：JP2014175220A
公开(公告)日：2014-09-22
申请号：JP2013048263
申请日：2013-03-11
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： YOSHINARI KIYOMI , TERUI YASUSHI
CPC classification number: H01J49/426 , H01J49/0031 , H01J49/0422 , H01J49/0431 , H01J49/4215 , H01J49/4225 , H01J49/429
Abstract: PROBLEM TO BE SOLVED: To provide a mass spectrometry system which allows for acquisition of mass spectrum with higher resolution for ions of higher mass number.SOLUTION: In a mass spectrometry system, a control unit 8 controls a mass analyzer 4 so that the DC voltage U, the high frequency voltage amplitude value V, and the high frequency voltage frequency f, being applied to a quadrupole electrode 13, are increased as the mass to charge ratio m/z of analyzed ions increases. Since the ion frequency of ions having a higher mass number increases more when passing through the mass analyzer 4, mass spectrum of higher resolution can be acquired.
Abstract translation: 要解决的问题：提供一种允许以更高质量的离子获得具有更高分辨率的质谱的质谱系统。解决方案：在质谱系统中，控制单元8控制质量分析器4，使得DC电压 U，施加到四极电极13的高频电压振幅值V和高频电压频率f随分析离子的质荷比m / z增加而增加。 由于在通过质量分析器4时质量分数较高的离子的离子频率增加，所以可以获得更高分辨率的质谱。
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公开(公告)号：JP2009115724A
公开(公告)日：2009-05-28
申请号：JP2007291460
申请日：2007-11-09
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： YOSHINARI KIYOMI , HIRABAYASHI TSUDOI , YOKOSUKA TOSHIYUKI , KOBAYASHI KINYA
IPC: G01N27/62
Abstract: PROBLEM TO BE SOLVED: To provide a mass spectrograph and a mass spectrometry capable of performing analysis in real time by reducing the number of parent ion species that should be dissociated for mass spectrometry.
SOLUTION: The mass spectrograph 1 has: a mass spectrometry section 13 for performing mass spectrometry of dissociated ions obtained by separating a substance included in a sample 10 and dissociating parent ions selected from ionized ion species; and a database 7 for storing the mass-to-charge ratio of ion species acquired by the mass spectrometry section 13 and characteristic data capable of identifying a plurality of ion species acquired by a preprocessing system. In the mass spectrometry section 13, the selection of parent ions and the mass spectrometry of dissociated ions are repeated by replacing the dissociated ions with ion species and parent ions that are not ion species that can be identified from the database 7 are selected. In the database 7, the mass-to-charge ratio of ion species where a substance labeled by a reagent has been ionized and characteristic data is reproduced, and the reproduced mass-to-charge ratio is rewritten from the increase/decrease in the number of masses when the substance is labeled by the reagent.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题：提供能够通过减少用于质谱分离的母离子种类的数量来实时进行分析的质谱仪和质谱仪。 质谱仪1具有：用于对通过分离样品10中包含的物质并离解选自离子化离子物质的亲本离子获得的离解离子进行质谱分析的质谱部13; 以及数据库7，用于存储由质谱分析部13获取的离子种类的质荷比和能够识别由预处理系统获取的多种离子种类的特征数据。 在质谱分析部分13中，通过用离子种类代替离解的离子并选择不能从数据库7中识别的离子种类的亲本离子，重复离子离子的选择和质谱分离。 在数据库7中，将通过试剂标记的物质离子化的离子物质的质荷比与再生特征数据进行比较，并且从数量的增加/减少重写质量 - 质荷比 当物质被试剂标记时。 版权所有（C）2009，JPO＆INPIT
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Patent Agency Ranking
公开(公告)号：JP2005091344A
公开(公告)日：2005-04-07
申请号：JP2004152693
申请日：2004-05-24
Applicant: Hitachi High-Technologies Corp , Hitachi Ltd , 株式会社日立ハイテクノロジーズ , 株式会社日立製作所
Inventor： YOSHINARI KIYOMI , YOKOSUKA TOSHIYUKI , SANO AKIHIRO , HIRABAYASHI TSUDOI , TERUI YASUSHI , KOBAYASHI KINYA , UCHIDA NORITAKA
CPC classification number: G01N33/6851 , G01N33/6848
Abstract: PROBLEM TO BE SOLVED: To avoid a peptide ion as a tandem mass spectrometry analysis target derived from the large quantity of a manifested protein previously measured, when structures of a protein and a peptide are analyzed by tandem mass spectrometry, and automatically determine and process the peptide as the tandem mass spectrometry analysis target derived from the small quantity of the protein conventionally difficult to be analyzed in real-time during measurement. SOLUTION: A process for selecting a peptide peak not measured as the next tandem analysis target is implemented in real time during the measurement, by automatically storing data of the protein previously measured and the peptide derived from it in an internal database, accurately comparing them with measured data and determining an isotope peak. The duplicated measurement of the peptide derived from the same protein is avoided. COPYRIGHT: (C)2005,JPO&NCIPI
Abstract translation: 要解决的问题：为了避免肽离子作为源自大量先前测量的表现蛋白质的串联质谱分析目标，当通过串联质谱法分析蛋白质和肽的结构时，并自动确定 并且作为来自在测量期间通常难以实时分析的少量蛋白质的串联质谱分析目标进行处理。 解决方案：在测量期间，通过自动将先前测量的蛋白质的数据和从其获得的肽准确地存储在内部数据库中，准确地实现了用于选择未被测量为下一个串联分析目标的肽峰的方法。 将其与测量数据进行比较并确定同位素峰。 避免了衍生自相同蛋白质的肽的重复测量。 版权所有（C）2005，JPO＆NCIPI
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公开(公告)号：JP2004245699A
公开(公告)日：2004-09-02
申请号：JP2003035928
申请日：2003-02-14
Applicant: Hitachi High-Technologies Corp , Hitachi Ltd , 株式会社日立ハイテクノロジーズ , 株式会社日立製作所
Inventor： YOSHINARI KIYOMI , KOBAYASHI KINYA , OTAKE ATSUSHI , OKUMOTO TOYOJI
CPC classification number: H01J49/26 , H01J49/0036 , H01J49/004
Abstract: PROBLEM TO BE SOLVED: To provide a mass spectrometry data analysis system, a mass spectrometry data analysis program, and a compound analysis system for precisely identifying a parent ion or estimating the structure of the parent ion by utilizing multistage dissociation mass spectrometry data (MS
n (n≥3) data), where no data are present on a database, when performing mass spectrometry by performing the multistage dissociation of the parent ion.
SOLUTION: A molecular orbital analysis, and molecular dynamic calculation are performed for the structure of a preestimated parent ion and a precursor ion at each dissociation stage, thus precisely deriving/displaying the structure of the parent ion and the structure of the precursor ion at each dissociation stage.
COPYRIGHT: (C)2004,JPO&NCIPI-
公开(公告)号：JP2003346706A
公开(公告)日：2003-12-05
申请号：JP2002156647
申请日：2002-05-30
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： BABA TAKASHI , HASHIMOTO YUICHIRO , YOSHINARI KIYOMI
CPC classification number: H01J49/424 , H01J49/004 , H01J49/401 , H01J49/427
Abstract: PROBLEM TO BE SOLVED: To solve the problem of an ion trap time-of-flight mass spectrometer method (IT-T OF MS) by thoroughly widening a mass window. SOLUTION: Heavy ion and light ion in a state of almost zero-energy are successively taken out in a sequence of weight, and accelerated by a prescribed voltage to guide to a pusher of a TOF spectrometric part. By applying a method of taking out the ion by forming an electric field gradient to the ion trap, and linearly reducing the high frequency voltage, a condition for dimensionally converging the ions of all kinds of mass can be detected. The TOF spectrometry is carried out by vertically accelerating the converged ions by the pusher. By the above, the ions having a wide range of mass number, requested at a protein analysis, can be analyzed by one shot of the TOF spectrometric operation. By the above, a high speed protein structure analysis is made possible. COPYRIGHT: (C)2004,JPO
Abstract translation: 要解决的问题：通过彻底扩大质量窗口来解决离子阱飞行时间质谱仪方法（IT-T OF MS）的问题。 解决方案：将几乎零能量状态的重离子和轻离子依次取出，并以规定的电压加速，以引导到TOF光谱测量部件的推动器。 通过应用通过对离子阱形成电场梯度而取出离子的方法，并且线性降低高频电压，可以检测出用于尺寸收敛各种质量的离子的条件。 TOF光谱法是通过推动器垂直加速会聚离子进行的。 通过上述，可以通过TOF光谱测量操作的一次分析在蛋白质分析中要求的具有宽范围质量数的离子。 通过上述，可以进行高速蛋白质结构分析。 版权所有（C）2004，JPO
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公开(公告)号：JP2010117377A
公开(公告)日：2010-05-27
申请号：JP2010044603
申请日：2010-03-01
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： YOSHINARI KIYOMI , YOKOSUKA TOSHIYUKI , SANO AKIHIRO , HIRABAYASHI TSUDOI , TERUI YASUSHI , KOBAYASHI KINYA , UCHIDA NORITAKA
CPC classification number: G01N33/6851 , G01N33/6848 , H01J49/0031 , H01J49/0036 , H01J49/004
Abstract: PROBLEM TO BE SOLVED: To avoid a peptide ion derived from a protein that has already been measured and that is expressed in great quantities as a tandem mass spectroscopy target, and to determine a peptide or the like derived from a minute amount of protein, which has heretofore been difficult to analyze as a tandem mass spectroscopy target, automatically within the real time of measurement, during the structural analysis of a protein or peptide by tandem mass spectroscopy. SOLUTION: Data concerning a protein that has already been measured and a peptide derived from the protein are automatically stored in an internal database. The stored data are collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. COPYRIGHT: (C)2010,JPO&INPIT
Abstract translation: 待解决的问题：为了避免衍生自已经测量并且作为串联质谱目标大量表达的蛋白质的肽离子，并且确定衍生自微量量的肽的肽等 蛋白质在蛋白质或肽通过串联质谱法的结构分析期间，在测量的实际时间内自动地被分析为串联质谱目标。 解决方案：已经测量的蛋白质和源自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据以高精度进行核对以确定同位素峰。 以这种方式，可以在测量的实时内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2008256667A
公开(公告)日：2008-10-23
申请号：JP2007204874
申请日：2007-08-07
Applicant: Hitachi High-Technologies Corp , 株式会社日立ハイテクノロジーズ
Inventor： HIRABAYASHI TSUDOI , ISHIMARU MASAKO , YOSHINARI KIYOMI , MARI NAOKI
Abstract: PROBLEM TO BE SOLVED: To provide a method for evaluating reliability of analysis data in the case where a quantitative analysis is carried out without using any isotopic labeling method. SOLUTION: An internal standard substance detected simultaneously with an analytical component is mixed in a mobile phase or an eluate of a liquid chromatograph, and a mass chromatogram of an ion derived from the internal standard substance is acquired and recorded by using a data analyzing section. Then, an analysis sample is mixed to obtain analysis data of the sample, and the strength of the ion derived from the internal standard substance is compared with that of a blank sample at an analysis actual time in the data analyzing section. If a miscompare is detected, such the judgement is made that a quantitative analysis inhibitory factor is generated, and its analysis mode is changed from a quantitative analysis mode in which the priority of a tandem mass spectrometry is low, to a qualitative analysis mode in which a high priority is put on the tandem mass spectrometry. If the strength of the ion derived from the internal standard substance matches with that of the blank sample in the analysis actual time, the analysis mode is changed into the quantitative analysis mode again. In a matching time zone, the analysis data are collected, and analyzing is carried out efficiently and precisely. COPYRIGHT: (C)2009,JPO&INPIT
Abstract translation: 要解决的问题：提供在不使用任何同位素标记方法进行定量分析的情况下评估分析数据的可靠性的方法。 解决方案：将与分析成分同时检测的内标物质在液相色谱仪的流动相或洗脱液中混合，通过使用数据获取并记录来自内标物质的离子的质谱图 分析部分。 然后，将分析样品混合，得到样品的分析数据，并在数据分析部分的分析实际时间将来自内标物质的离子的强度与空白样品的强度进行比较。 如果检测到错误比较，则判断产生了定量分析抑制因子，并且将其分析模式从串联质谱的优先级的定量分析模式改变为定性分析模式，其中 高优先级的串联质谱法。 如果在分析实际时间内，由内标物质衍生的离子强度与空白样品的强度相符，则分析模式再次变为定量分析模式。 在匹配的时区，收集分析数据，并进行有效和准确的分析。 版权所有（C）2009，JPO＆INPIT
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