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1.发明专利
公开(公告)号：JP2006068011A
公开(公告)日：2006-03-16
申请号：JP2005246323
申请日：2005-08-26
Inventor： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
CPC classification number: C12Q1/6851
Abstract: PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: This method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme, and then calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding background fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。 解决方案：用于从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或扩增 对应于不包括背景荧光信号的最大荧光信号的强度的一半，对应于最大扩增效率的放大循环次数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。 因此，可以不使用分化/积分法来确定核酸的早期浓度。 版权所有（C）2006，JPO＆NCIPI
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2.发明专利Fet base sensor for ion material detection, detection device of ion material equipped therewith, and detection method of ion material utilizing it 审中-公开用于离子材料检测的FET基座传感器，其配备的离子材料的检测装置以及利用其的离子材料的检测方法
公开(公告)号：JP2007183259A
公开(公告)日：2007-07-19
申请号：JP2006332288
申请日：2006-12-08
Inventor： LEE KYU-SANG , YOO KYU-TAE , SHIM JEO-YOUNG , KIM JIN-TAE , CHO YEON-JA
IPC: G01N27/414 , C12M1/00 , C12N15/09 , G01N27/26
CPC classification number: G01N33/54373 , C12Q1/6825 , G01N27/4145
Abstract: PROBLEM TO BE SOLVED: To provide an FET base sensor for ion material detection having high sensitivity, a detection device of the ion material equipped therewith, and a detection method of the ion material utilizing it.
SOLUTION: This sensor is equipped with a sensing chamber 10 equipped with one reference electrode 14 and a plurality of sensing FETs 12, and a reference chamber 11 equipped with another reference electrode 14 and a plurality of reference FETs 13.
COPYRIGHT: (C)2007,JPO&INPITAbstract translation: 要解决的问题：提供一种具有高灵敏度的离子材料检测用FET基传感器，配备有离子材料的检测装置以及利用该离子材料的检测方法。 解决方案：该传感器配备有一个传感室10，它具有一个参考电极14和多个检测FET 12，以及一个配有另一参考电极14和多个参考FET 13的参考室11。 版权所有（C）2007，JPO＆INPIT
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公开(公告)号：JP2008116446A
公开(公告)日：2008-05-22
申请号：JP2007268238
申请日：2007-10-15
Inventor： KIM SU-HYEON , KIM JIN-TAE
CPC classification number: G01N21/645 , G01N2021/6482
Abstract: PROBLEM TO BE SOLVED: To provide an ultrasmall fluorescence detector for detecting a reaction in a prescribed volume of microchamber of a microifine fluid chip, in a short time. SOLUTION: This fluorescence detector for measuring PCR amplification in the microifine fluid chip includes the prescribed volume of micro chamber in an actual time includes a sample flow-in port, a sample discharge port, a micro channel, the microifine fluid chip having the wide microchamber, a microheater for regulating a reaction temperature in the microchamber, a light emitting diode light source for excitation light, the first optical system mechanism for irradiating the microchamber with the excitation light, the first detector, and the second optical system mechanism for reflecting a guided fluorescent beam toward the first detector in the microchamber. The light from the light source is focused between the first mirror and an objective lens to be converted into a spot size irradiating the whole micro chamber, the spot size of the excitation light passed through the objective lens is formed widely to irradiate the whole microchamber of the microifine fluid chip with the excitation light, and the fluorescent beam is detected thereby in a wide area. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 要解决的问题：提供一种用于在短时间内检测微量流体芯片的规定体积的微室中的反应的超小型荧光检测器。 解决方案：用于测量微流体芯片中的PCR扩增的荧光检测器包括在实际时间内的规定体积的微室包括样品流入口，样品排出口，微通道，微流体芯片具有 宽微室，用于调节微室中的反应温度的微加热器，用于激发光的发光二极管光源，用于用激发光照射微室的第一光学系统机构，第一检测器和第二光学系统机构， 将引导的荧光束反射到微室中的第一检测器。 来自光源的光聚焦在第一反射镜和物镜之间，以转换成照射整个微室的光斑尺寸，通过物镜的激发光的光斑尺寸广泛地形成以照射整个微室 具有激发光的微流体芯片，并且在广泛的区域中检测荧光束。 版权所有（C）2008，JPO＆INPIT
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公开(公告)号：JP2006158393A
公开(公告)日：2006-06-22
申请号：JP2005341097
申请日：2005-11-25
Inventor： CHO YOON-KYOUNG , LEE KYU-SANG , KIM SOOK-YOUNG , KIM JIN-TAE
CPC classification number: C12N15/1006 , C12Q1/6806 , C12Q1/70 , Y10S977/924 , C12Q2563/155 , C12Q2523/308
Abstract: PROBLEM TO BE SOLVED: To provide a method for purifying a nucleic acid by using silver nanoparticles. SOLUTION: The method for purifying a target substance using silver nanoparticles comprises (a) a step to mix a specimen containing a molecule having a thiol group with silver nanoparticles to form a mixture containing a complex of the molecule and the silver nanoparticles and (b) a step to separate and remove the complex from the mixture. The method is extremely useful in LOC compared with conventional nucleic acid purification method because the method enables the DNA purification enabling quick PCR amplification by a three-stage operation even if including a work to dissolve the specimen containing a molecule having a thiol group. COPYRIGHT: (C)2006,JPO&NCIPI
Abstract translation: 待解决的问题：提供通过使用银纳米粒子来纯化核酸的方法。 解决方案：使用银纳米颗粒纯化目标物质的方法包括（a）将含有硫醇基的分子的样品与银纳米颗粒混合以形成含有分子和银纳米颗粒络合物的混合物的步骤，以及 （b）从混合物中分离并除去复合物的步骤。 与常规核酸纯化方法相比，该方法在LOC中非常有用，因为即使包括溶解含有具有硫醇基的分子的样品的工作，该方法也能够通过三级操作实现快速PCR扩增的DNA纯化。 版权所有（C）2006，JPO＆NCIPI
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5.发明专利Light-detecting device for multichannel and multicolor measurement, and multichannel sample analyzer adopting the same 有权用于多通道和多重测量的光检测装置，以及采用该方法的多通道样品分析仪
公开(公告)号：JP2006078481A
公开(公告)日：2006-03-23
申请号：JP2005252610
申请日：2005-08-31
Inventor： OK GYEONG-SIK , KIM JIN-TAE , OH KWANG-WOOK , KIM SANG-HYO
CPC classification number: G01N21/645 , G01J3/51 , G01N2021/6421 , G01N2021/6471
Abstract: PROBLEM TO BE SOLVED: To provide a light detecting device for multichannel and multicolor measurement and a multichannel sample analyzer that adopts it.
SOLUTION: The light detecting device comprises a photodetector 10, a filter wheel 12 where at least two color filters are mutually bonded disk-like, a plurality of optical channels 17 for making a plurality of beams enter the filter wheel 12, and a mirror portion 20 where a plurality of mirrors for sequentially reflecting a plurality of the beams that pass through the filter wheel 12, to the photodetector 10, respectively. The mirror portion 20 rotates with the filter wheel 12.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 要解决的问题：提供多通道和多色测量的光检测装置和采用它的多通道样品分析仪。 光检测装置包括光电检测器10，滤光轮12，其中至少两个滤色器相互贴合盘状，多个光通道17用于使多个光束进入滤光轮12，以及 反射镜部分20，其中分别将通过滤光轮12的多个光束顺序地反射的多个反射镜分别连接到光电检测器10。 镜部分20与滤光轮12一起旋转。（C）2006，JPO＆NCIPI
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Patent Agency Ranking6.发明专利Optical system for multi-channel fluorometry, and analyzer for multi-channel fluorescence sample adopting same 审中-公开用于多通道荧光分析的光学系统和用于多通道荧光样品的分析仪
公开(公告)号：JP2006215026A
公开(公告)日：2006-08-17
申请号：JP2006011741
申请日：2006-01-19
Inventor： OK GYEONG-SIK , KIM SU-HYEON , KIM JIN-TAE , OH KWANG-WOOK
IPC: G01N21/64
CPC classification number: G01J3/10 , G01J3/02 , G01J3/0218 , G01J3/0256 , G01J3/4406 , G01J2003/1213 , G01N21/05 , G01N21/6452 , G01N21/6456 , G01N2021/0346 , G01N2021/3174 , G01N2021/6419 , G01N2021/6421 , G01N2021/6471 , G01N2021/6484 , G01N2201/0221 , G01N2201/0407 , G01N2201/0461 , G01N2201/0612 , G01N2201/0627 , G01N2201/0631 , G01N2201/0806 , G01N2201/0833
Abstract: PROBLEM TO BE SOLVED: To provide an optical system for multi-channel fluorometry. SOLUTION: This optical system for the multi-channel fluorometry for emitting light to a plurality of sample channels to detect fluorescence emitted from samples includes a light source 10; an integrator 20 for bringing the light emitted from the light source into light having a uniform intensity distribution; a sample holder 30 provided with the plurality of sample channels for mounting the samples fluorescence-reacting with the light emitted from the integrator; and a beam splitter 33 arranged between the integrator and the sample holder to separate incident light at a prescribed ratio. COPYRIGHT: (C)2006,JPO&NCIPI
Abstract translation: 要解决的问题：提供用于多通道荧光测定的光学系统。 解决方案：用于将光发射到多个样品通道以检测从样品发射的荧光的用于多通道荧光测定的光学系统包括光源10; 用于将从光源发射的光引入具有均匀强度分布的光的积分器20; 设置有多个样品通道的样品保持器30，用于安装与从积分器发射的光发生荧光反应的样品; 以及布置在积分器和样品架之间的分束器33，以以规定的比例分离入射光。 版权所有（C）2006，JPO＆NCIPI
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7.发明专利
公开(公告)号：JP2009106298A
公开(公告)日：2009-05-21
申请号：JP2008313681
申请日：2008-12-09
Inventor： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
CPC classification number: C12Q1/6851
Abstract: PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises: a step of amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme; a step of generating a function representing correlation with strength of fluorescent signal by amplification amount shown in real time by amplification cycle number or amplification time of the nucleic acid; a step of calculating a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals of the nucleic acid by utilizing the function; and a step of obtaining initial concentration of the nucleic acid from the calculated unamplified fluorescent light signal strength. Thereby, the early concentration of the nucleic acid can be determined without using a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。 解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括：用酶扩增从生物体或病毒提取的核酸（DNA或RNA）的步骤; 通过扩增循环次数或核酸扩增时间实时显示荧光信号强度的相关性函数的步骤; 通过利用该功能计算除了核酸的背景荧光信号之外的未扩增核酸荧光信号的强度的步骤; 以及从计算的未扩增的荧光信号强度获得核酸的初始浓度的步骤。 因此，可以不使用分化/积分法来确定核酸的早期浓度。 版权所有（C）2009，JPO＆INPIT
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8.发明专利
公开(公告)号：JP2009072206A
公开(公告)日：2009-04-09
申请号：JP2008313682
申请日：2008-12-09
Inventor： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
CPC classification number: C12Q1/6851
Abstract: PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data includes amplifying a nucleic acids (DNA or RNA) extracted from an organism or virus with an enzyme, and then, calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding back ground fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。 解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或 对应于不包括背景荧光信号的最大荧光信号的强度的一半的放大时间，对应于最大扩增效率的扩增循环数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。 因此，可以不使用分化/积分法来确定核酸的早期浓度。 版权所有（C）2009，JPO＆INPIT
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9.发明专利Microfluidic system comprising microchannel in which a plurality of electromagnets are arranged, and methods of mixing sample and dissolving cell with the microfluidic system 审中-公开微电子系统包含多个电磁网络的微通道，以及混合样品和溶解电池与微流控系统的方法
公开(公告)号：JP2006142294A
公开(公告)日：2006-06-08
申请号：JP2005332185
申请日：2005-11-16
Inventor： KIM JIN-TAE , OH KWANG-WOOK , CHO YOON-KYOUNG , PAEK SANGHYUN , KIM SOOK-YOUNG , PARK CHIN-SUNG , NAMKOONG KAK
CPC classification number: C12N1/066 , B01F13/0059 , B01F13/0809
Abstract: PROBLEM TO BE SOLVED: To provide a microfluidic system comprising a microchannel in which a plurality of electromagnets are arranged, and methods of mixing a sample and dissolving a cell with the microfluidic system.
SOLUTION: There is provided a microfluidic system comprising at least an inlet and at least an outlet, the inlet and the outlet being connected through a microchannel 400, wherein two or more electromagnets are arranged in a predetermined direction to a wall surface of the microchannel 400 relative to the proceeding direction of a fluid passing through the microchannel 400.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 要解决的问题：提供一种微流体系统，其包括其中布置有多个电磁体的微通道，以及混合样品并将细胞溶解于微流体系统的方法。 解决方案：提供了一种微流体系统，其至少包括入口和至少一个出口，入口和出口通过微通道400连接，其中两个或多个电磁体沿预定方向布置在 微通道400相对于通过微通道400的流体的行进方向。（C）2006年，JPO和NCIPI
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10.发明专利Optical system for analyzing multi-channel sample and multi-channel sample analyzer using it 有权用于分析多通道样品的光学系统和使用其的多通道样品分析仪
公开(公告)号：JP2006084465A
公开(公告)日：2006-03-30
申请号：JP2005256561
申请日：2005-09-05
Inventor： OK GYEONG-SIK , CHO YOON-KYOUNG , KIM JIN-TAE , OH KWANG-WOOK
IPC: G01N21/64
CPC classification number: G01N21/645
Abstract: PROBLEM TO BE SOLVED: To provide an optical system for analyzing multi-channel samples which uses a high-speed rotating mirror and an aspherical mirror, and a multi-channel analyzer using the optical system.
SOLUTION: This optical system comprises: a light source unit 10 emitting light traveling along an optical axis; a semi-spheroid aspherical mirror 30 disposed in rotational symmetry about the optical axis; and an inclined mirror 41 reflecting the light exiting the light source unit to the semi-spheroid aspherical mirror, while rotating about the optical axis, wherein an opening is formed in the center of the semi-spheroid aspherical mirror so that the light emitted from the light source unit can enter the inclined mirror through the semi-spheroid aspherical mirror. According to this, a plurality of samples are measured while preventing optical strokes between the samples, and parts to be used are miniaturized.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 解决的问题：提供一种用于分析使用高速旋转镜和非球面镜的多通道样本的光学系统和使用该光学系统的多通道分析仪。 解决方案：该光学系统包括：光源单元10，其发射沿着光轴行进的光; 围绕光轴旋转对称设置的半球体非球面镜30; 以及在围绕光轴旋转的同时将离开光源单元的光反射到半球形非球面镜的倾斜镜41，其中在半球体非球面镜的中心形成有开口，使得从 光源单元可以通过半球体非球面镜进入倾斜镜。 据此，在防止样品之间的光笔冲击的同时测量多个样品，并且使用的部件被小型化。 版权所有（C）2006，JPO＆NCIPI
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