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    Detection method and reagent for detection and red blood cell membrane partial dissolution reagent used in this malaria-infected red blood cells
    1.
    发明专利
    Detection method and reagent for detection and red blood cell membrane partial dissolution reagent used in this malaria-infected red blood cells 有权

    公开(公告)号：JP4969115B2

    公开(公告)日：2012-07-04

    申请号：JP2006051981

    申请日：2006-02-28

    申请人： シスメックス株式会社

    发明人： 欣也 内橋 ,  歩 吉田 ,  綾 小西 ,  裕司 糸瀬 ,  啓光 飯塚

    IPC分类号： C12Q1/04 ,  G01N33/48

    CPC分类号： Y02A50/58

    摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a malaria parasite in which malaria-infected red blood cell can simply and rapidly be detected and falciparum malaria can be discriminated from other malaria and infection rate can be determined and to provide a reagent for detection used for the method and to provide a reagent for partial lysis of cell membrane of red blood cell. SOLUTION: The reagent for partial lysis of a cell membrane of a red blood cell infected with a malaria parasite comprises a first surfactant which has predetermined lysing ability with respect to the red blood cell membrane and a second surfactant which has weaker lysing ability than that of the first surfactant; wherein the reagent has a pH of 5 to 7 and an osmotic pressure of 200 to 300 mOsm/(kg H 2 O); and wherein the reagent partially lyses the cell membrane of the malaria infected red blood such that a malaria parasite is retained in the red blood cell and fluorescent dye transmits the cell membrane. The malaria infected red blood cell is detected from scattered light information and fluorescent information obtained by introducing a sample in which the detection reagent is mixed with a whole blood specimen into a flow cytometer. COPYRIGHT: (C)2007,JPO&INPIT

    Method, apparatus and reagent for measuring malaria-infected erythrocyte, and method, apparatus and reagent for measuring plasmodium
    3.
    发明专利
    Method, apparatus and reagent for measuring malaria-infected erythrocyte, and method, apparatus and reagent for measuring plasmodium 审中-公开
    用于测量疟疾感染性红细胞的方法，装置和试剂，以及用于测量疟原虫的方法，装置和试剂

    公开(公告)号：JP2006101708A

    公开(公告)日：2006-04-20

    申请号：JP2004289028

    申请日：2004-09-30

    申请人： Sysmex Corp ,  シスメックス株式会社

    发明人： ITOSE YUUJI ,  OKITSU NAOYA ,  KONISHI AYA

    IPC分类号： C12Q1/02 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/78 ,  G01N33/48 ,  G01N33/50

    CPC分类号： Y02A50/58

    摘要： PROBLEM TO BE SOLVED: To detect malaria-infected erythrocytes accurately and simply. SOLUTION: A method for detecting the malaria-infected erythrocytes is provided by adding an anthraquinone-based fluorescent pigment dyeing DNA specifically to a blood sample to prepare a specimen for measurement, detecting an optical information from the prepared specimen for measurement and detecting the malaria-infected erythrocytes based on the detected optical information. COPYRIGHT: (C)2006,JPO&NCIPI

    摘要翻译： 要解决的问题：准确，简单地检测疟疾感染的红细胞。 解决方案：通过将基于蒽醌的荧光颜料染色DNA特异性地添加到血液样品中以制备测量样本来提供用于检测疟疾感染的红细胞的方法，从所制备的样品中检测来自测量和检测的光学信息 基于检测到的光学信息的疟疾感染的红细胞。 版权所有（C）2006，JPO＆NCIPI

    Method for detecting mutation of cyp2c19, and nucleic acid probe therefor
    4.
    发明专利
    Method for detecting mutation of cyp2c19, and nucleic acid probe therefor 有权
    检测CYP2C19的突变体及其核酸探针的方法

    公开(公告)号：JP2005058114A

    公开(公告)日：2005-03-10

    申请号：JP2003293702

    申请日：2003-08-15

    申请人： Arkray Inc ,  アークレイ株式会社

    发明人： INOSE TAKESHI

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/542 ,  G01N33/58 ,  G01N37/00

    CPC分类号： Y02A50/58

    摘要： PROBLEM TO BE SOLVED: To provide a method for detecting mutation (G681A mutation) at the 681 position of the cDNA of a CYP2C19 gene. SOLUTION: The method for detecting the mutation comprises carrying out a melting curve analysis by amplifying a region containing a G681A mutation by a PCR, using a nucleic acid probe having a terminus labeled with a fluorochrome, regulated so that the fluorescence may be reduced when hybridized, having a base sequence complementary to a base sequence started from the base number 189 in a specific base sequence and having 20-35 base length, and further having the 3'-terminus labeled with the fluorochrome, and measuring the fluorescence of the fluorochrome, and detecting the mutation based on the result of the melting curve analysis. COPYRIGHT: (C)2005,JPO&NCIPI

    摘要翻译： 待解决的问题：提供在CYP2C19基因的cDNA的681位置检测突变（G681A突变）的方法。 检测突变的方法包括通过使用具有用荧光染料标记的末端的核酸探针通过PCR扩增含有G681A突变的区域来进行解链曲线分析，使得荧光可以是 杂交时减少，具有与碱基序列互补的碱基序列，其碱基序列从碱基序列189开始并具有20-35碱基长度，并进一步具有用荧光染料标记的3'末端，并测量荧光 荧光染料，并根据熔解曲线分析的结果检测突变。 版权所有（C）2005，JPO＆NCIPI

    Method for synthesizing highly sulfated chondroitin sulfates, highly sulfated chondroitin sulfates, and reagent for analysis
    9.
    发明专利
    Method for synthesizing highly sulfated chondroitin sulfates, highly sulfated chondroitin sulfates, and reagent for analysis 有权
    用于合成高度硫化的硫酸氢醌的方法，高硫酸化的硫酸铁和用于分析的试剂

    公开(公告)号：JP2012157271A

    公开(公告)日：2012-08-23

    申请号：JP2011018629

    申请日：2011-01-31

    申请人： Aichi Medical Univ ,  学校法人 愛知医科大学

    发明人： SUGIURA NOBUO

    IPC分类号： C12P19/26 ,  A61K49/00 ,  C08B37/08 ,  G01N33/536 ,  G01N33/566

    CPC分类号： Y02A50/58

    摘要： PROBLEM TO BE SOLVED: To provide a method for synthesizing highly sulfated chondroitin sulfates.SOLUTION: The method for synthesizing highly sulfated chondroitin sulfates includes: a step (A1) of forming A structure of a chondroitin sulfate by making sulfotransferase (C4ST-1) act on the chondroitin; a step (A2) of forming E structure of a chondroitin sulfate by making a sulfotransferase (Ga1NAc4S-6ST) act on the A structure; and a step (A3) of forming triS structure of a chondroitin sulfate by making a sulfotransferase UST act on the E structure.

    摘要翻译： 待解决的问题：提供一种合成高度硫酸化硫酸软骨素的方法。 解决方案：高硫酸化硫酸软骨素合成方法包括：通过使磺基转移酶（C4ST-1）作用于软骨素上形成硫酸软骨素A结构的步骤（A1） 通过使磺酰转移酶（Ga1NAc4S-6ST）作用于A结构而形成硫酸软骨素的E结构的步骤（A2） 以及通过使磺化转移酶UST作用于E结构而形成硫酸软骨素的三硫结构的步骤（A3）。 版权所有（C）2012，JPO＆INPIT

    Nucleic acid detection method and apparatus, as well as nucleic acid detection for container
    10.
    发明专利
    Nucleic acid detection method and apparatus, as well as nucleic acid detection for container 有权

    公开(公告)号：JP3677237B2

    公开(公告)日：2005-07-27

    申请号：JP2001367484

    申请日：2001-11-30

    申请人： 株式会社東芝

    发明人： 幸二 橋本

    IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68

    CPC分类号： Y02A50/58

    摘要： PROBLEM TO BE SOLVED: To provide a method and an apparatus for detecting nucleic acids useful when the detection of the many species of nucleic acids handling a plurality of samples is readily carried out with a high accuracy in simple equipment, and a container for detecting the nucleic acids. SOLUTION: This apparatus for detecting the nucleic acids is equipped with a nucleic acid-immobilized electrode in which nucleic acid probes are immobilized on a conductor, a plurality of containers for bringing the nucleic acid probes into contact with a test substance, a counter electrode installed at the base or the inner surface of each container and an electric circuit for applying a voltage across the nucleic acid-immobilized electrode and the counter electrode. The nucleic acid-immobilized electrode is inserted into each container in which the test substance is poured to detect the nucleic acids having a specific sequence while electrically controlling the reaction using the counter electrode at the base of the container.