酵素含有液体培養方法及び酵素含有粉末の製造方法

    公开(公告)号:JP2018134029A

    公开(公告)日:2018-08-30

    申请号:JP2017030194

    申请日:2017-02-21

    Inventor: 森 慎一郎

    Abstract: 【課題】菌増殖を目的とした前培養を行うことを必要としない酵素含有液体培養、及び、難消化性糖質を液体培地中に使用せずに酵素を生産できる酵素含有液体培養方法の提供。 【解決手段】調整液体培地に第一の穀類麹を添加すること、第一の穀類麹を添加した調整液体培地を用いて第一の通気培養を行うこと、第一の通気培養を行った調整液体培地に第二の穀類麹を添加すること、及び第二の穀類麹を添加した調整液体培地を用いて第二の通気培養を行うこと、を含む酵素含有液体培養方法。好ましくは、第一の穀類麹が、玄米麹、大豆麹、米糠麹、粟麹又は稗麹から一つ以上選定され、第二の穀類麹が、大豆麹である、酵素含有液体培養方法。 【選択図】なし

    Method of manufacturing a filamentous fungus culture

    公开(公告)号:JP4966346B2

    公开(公告)日:2012-07-04

    申请号:JP2009192762

    申请日:2009-08-24

    Abstract: It is an object of the present invention to provide a method of adjusting productivity of enzymes, in particular, amylolytic enzymes, plant fiber degradation enzymes and proteolytic enzymes in a filamentous fungus culture product, by controlling releasing rate of nutrients from the culture raw material into the culture system when a filamentous fungus culture product is produced by culturing filamentous fungi in liquid medium containing as the culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa. The present invention provides a method of producing filamentous fungus culture product by using liquid medium containing as culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa comprising; culturing filamentous fungi while releasing rate of nutrients from the culture raw material into the culture system is controlled to adjust productivity of enzymes in the filamentous fungus culture product.

    Method for producing protein by continuous fermentation
    10.
    发明专利
    Method for producing protein by continuous fermentation 有权
    通过连续发酵生产蛋白质的方法

    公开(公告)号:JP2009039074A

    公开(公告)日:2009-02-26

    申请号:JP2007209936

    申请日:2007-08-10

    CPC classification number: Y02P20/52

    Abstract: PROBLEM TO BE SOLVED: To provide a method for producing a protein by continuous fermentation under simple operational conditions so as to maintain its high productivity stably for a long period of time. SOLUTION: The method for producing a protein by continuous fermentation process comprises separating a fermented culture liquid of microorganisms having the ability to secretorily produce the protein into a filtrate and an unfiltrate through separation membranes and retrieving the protein as the desired fermentation product while keeping or refluxing the unfiltrate in or to the fermented culture liquid. In this method, specific porous membranes with both high permeability and cell-blocking ability and high plugging resistance are used as the separation membranes, and a filtration treatment is conducted at specific low intermembrane differential pressure, thus enabling the production efficiency of the objective protein to be markedly improved stably at low cost by fermentation. COPYRIGHT: (C)2009,JPO&INPIT

    Abstract translation: 待解决的问题:提供在简单的操作条件下通过连续发酵来生产蛋白质的方法,以便长时间稳定地保持其高生产率。 解决方案:通过连续发酵方法生产蛋白质的方法包括将具有分泌蛋白质的能力的微生物发酵培养液分离成滤液和通过分离膜进行未过滤,并将蛋白质作为所需发酵产物回收,同时 将未滤过液保持或回流到发酵培养液中或加到发酵培养液中。 在该方法中,使用具有高渗透性和细胞阻断能力和高耐堵塞性的特定多孔膜作为分离膜,并且在特定的低膜间差压下进行过滤处理,从而使目标蛋白的生产效率 通过发酵以低成本稳定地显着地改善。 版权所有(C)2009,JPO&INPIT

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