公开(公告)号：US20140044866A1

公开(公告)日：2014-02-13

申请号：US13979129

申请日：2012-01-10

Applicant: Jan Behrends ,  Gerhard Baaken

Inventor： Jan Behrends ,  Gerhard Baaken

IPC: B81C1/00

CPC classification number: B05D1/40 ,  B05C11/023 ,  B05C11/08 ,  B81C1/0038 ,  G01N33/5076

Abstract: The invention is related to a method and an apparatus for the automated fabrication of a molecular layer made from amphiphilic molecules, in particular lipids for a lipid bilayer membrane, in the apparatus, which has a support substrate for supporting the molecular layer, a rotation element, which can be rotated on top of the support substrate, and an actuator device, by means of which the rotation element can be automatically rotated, wherein the rotation element has an outer surface, which is formed hydrophobic at least in sections, the method having the following steps: placing a first solvent, which contains amphiphilic molecules, in a region above the support substrate; causing the automatic rotation of the rotation element above the support substrate; moving the first solvent between the support substrate and the rotation element by the interaction of the rotating rotation element with the first solvent, thus forming the molecular layer.

Abstract translation: 本发明涉及一种用于自动制造由两亲分子，特别是用于脂双层膜的脂质制成的分子层的方法和装置，该装置具有用于支撑分子层的支撑基底，旋转元件 ，其可以在支撑基板的顶部上旋转;以及致动器装置，借助于该致动器装置，旋转元件可以自动旋转，其中旋转元件具有外表面，该外表面至少部分地形成为疏水性，该方法具有 以下步骤：将包含两亲分子的第一溶剂放置在载体基材上方的区域中; 使旋转元件自动旋转在支撑基板上方; 通过旋转旋转元件与第一溶剂的相互作用，在支撑基板和旋转元件之间移动第一溶剂，从而形成分子层。

公开(公告)号：US09403184B2

公开(公告)日：2016-08-02

申请号：US13979129

申请日：2012-01-10

Applicant: Jan Behrends ,  Gerhard Baaken

Inventor： Jan Behrends ,  Gerhard Baaken

IPC: B05D1/40 ,  G01N33/50 ,  B81C1/00 ,  B05C11/08 ,  B05C11/02

CPC classification number: B05D1/40 ,  B05C11/023 ,  B05C11/08 ,  B81C1/0038 ,  G01N33/5076

Abstract: The invention is related to a method and an apparatus for the automated fabrication of a molecular layer made from amphiphilic molecules, in particular lipids for a lipid bilayer membrane, in the apparatus, which has a support substrate for supporting the molecular layer, a rotation element, which can be rotated on top of the support substrate, and an actuator device, by means of which the rotation element can be automatically rotated, wherein the rotation element has an outer surface, which is formed hydrophobic at least in sections, the method having the following steps: placing a first solvent, which contains amphiphilic molecules, in a region above the support substrate; causing the automatic rotation of the rotation element above the support substrate; moving the first solvent between the support substrate and the rotation element by the interaction of the rotating rotation element with the first solvent, thus forming the molecular layer.

Abstract translation: 本发明涉及一种用于自动制造由两亲分子，特别是用于脂双层膜的脂质制成的分子层的方法和装置，该装置具有用于支撑分子层的支撑基底，旋转元件 ，其可以在支撑基板的顶部上旋转;以及致动器装置，借助于该致动器装置，旋转元件可以自动旋转，其中旋转元件具有外表面，该外表面至少部分地形成为疏水性，该方法具有 以下步骤：将包含两亲分子的第一溶剂放置在载体基材上方的区域中; 使旋转元件自动旋转在支撑基板上方; 通过旋转旋转元件与第一溶剂的相互作用，在支撑基板和旋转元件之间移动第一溶剂，从而形成分子层。

公开(公告)号：US09671441B2

公开(公告)日：2017-06-06

申请号：US13979128

申请日：2012-01-10

Applicant: Jan Behrends ,  Gerhard Baaken ,  Juergen Ruehe ,  Martin Vellinger

Inventor： Jan Behrends ,  Gerhard Baaken ,  Juergen Ruehe ,  Martin Vellinger

IPC: G01N27/327 ,  G01R27/00 ,  G01N33/487

CPC classification number: G01R27/00 ,  G01N33/48728

Abstract: The invention is related to a microstructure apparatus for the measurement of biological membranes, comprising a support substrate having an upper side for supporting the membrane, at least one microcavity of the support substrate for receiving an electrolyte, wherein the microcavity is open upward and ends in a microaperture in the upper side of the support substrate, wherein the microaperture has a first characteristic diameter D1 and has at least one electrode, which is at least partially arranged within the microcavity and which has a contact side for contacting an electrolyte, the contact side being arranged adjacent to the inner volume of the microcavity, characterized in that the contact side of the electrode has a characteristic diameter D2, being larger than D1. The invention further relates to a corresponding method for producing the microstructure apparatus.

公开(公告)号：US20140062503A1

公开(公告)日：2014-03-06

申请号：US13979128

申请日：2012-01-10

Applicant: Jan Behrends ,  Gerhard Baaken ,  Juergen Ruehe ,  Martin Vellinger

Inventor： Jan Behrends ,  Gerhard Baaken ,  Juergen Ruehe ,  Martin Vellinger

IPC: G01R27/00

CPC classification number: G01R27/00 ,  G01N33/48728

Abstract: The invention is related to a microstructure apparatus for the measurement of biological membranes, comprising a support substrate having an upper side for supporting the membrane, at least one microcavity of the support substrate for receiving an electrolyte, wherein the microcavity is open upward and ends in a microaperture in the upper side of the support substrate, wherein the microaperture has a first characteristic diameter D1 and has at least one electrode, which is at least partially arranged within the microcavity and which has a contact side for contacting an electrolyte, the contact side being arranged adjacent to the inner volume of the microcavity, characterized in that the contact side of the electrode has a characteristic diameter D2, being larger than D1. The invention further relates to a corresponding method for producing the microstructure apparatus.

Abstract translation: 本发明涉及一种用于测量生物膜的微结构装置，包括：具有用于支撑膜的上侧的支撑基板，用于容纳电解质的支撑基板的至少一个微腔，其中微腔向上敞开并以 微支架，其中所述微孔具有第一特征直径D1，并且具有至少一个电极，所述至少一个电极至少部分地布置在所述微腔内并且具有用于接触电解质的接触侧，所述接触侧 被布置成邻近微腔的内部体积，其特征在于，电极的接触侧具有大于D1的特征直径D2。 本发明还涉及用于生产微结构装置的相应方法。

公开(公告)号：US20130140192A1

公开(公告)日：2013-06-06

申请号：US13702802

申请日：2011-06-01

Applicant: Jan Behrends ,  Gerhard Baaken

Inventor： Jan Behrends ,  Gerhard Baaken

IPC: G01N27/22

CPC classification number: G01N27/22 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/16 ,  B01L2300/165 ,  G01N33/48728

Abstract: The present invention relates to a method of producing a lipid bilayer over a microcavity open on one side and to a microstructure for investigating lipid bilayers and an associated measuring arrangement. The method of producing a lipid bilayer over a microcavity open on one side comprises the following steps: filling the microcavity with an electrolyte solution; moving a fluid containing dissolved lipids in a first direction onto the microcavity; moving the fluid in a second direction away from the microcavity; monitoring the formation of the lipid bilayer over the microcavity by detecting an impedance between a counter-electrode connected to the fluid and a measuring electrode, which is arranged inside the microcavity. The microstructure has a substrate, in which at least one microcavity is formed, wherein at least one measuring electrode is arranged inside the microcavity and wherein the at least one microcavity can be connected to a fluid channel so that a laminar flow of fluid can be made to flow over the microcavity with at least two different directions of flow.

Abstract translation: 本发明涉及在一侧开放的微腔上制备脂质双层的方法以及用于研究脂质双层的微结构和相关的测量装置。 在一侧开放的微腔上制备脂质双层的方法包括以下步骤：用电解质溶液填充微腔; 将含有溶解脂质的流体沿第一方向移动到微腔上; 将流体沿远离微腔的第二方向移动; 通过检测连接到流体的对电极和布置在微腔内的测量电极之间的阻抗来监测微腔上的脂质双层的形成。 所述微结构具有基板，其中形成有至少一个微腔，其中至少一个测量电极布置在所述微腔内部，并且其中所述至少一个微腔可连接到流体通道，使得能够形成流体的层流 以至少两个不同的流动方向流过微腔。