公开(公告)号：US5047506A

公开(公告)日：1991-09-10

申请号：US321133

申请日：1989-03-09

申请人： Hartmut Lobermann ,  Jurgen Romisch ,  Werner Stuber

发明人： Hartmut Lobermann ,  Jurgen Romisch ,  Werner Stuber

IPC分类号： A61K38/43 ,  A61K38/00 ,  A61P7/06 ,  C07K1/113 ,  C07K1/22 ,  C07K14/435 ,  C07K14/745 ,  C07K14/755 ,  C12N9/10

CPC分类号： C12N9/1044 ,  A61K38/00

摘要： The invention relates to a method for the purification of the a subunit of factor XIII by affinity chromatography, to a therapeutic composition containing the latter, and to the use of the therapeutic composition.Factor XIII has hitherto been purified either by methods which are technically very elaborate or else by use of toxic affinity chromatography materials. The invention has the aim of providing an improved method for the purification of the a subunit of factor XIII.Factor XIII is obtained according to the invention by a method in which the a subunit of factor XIII is reversibly bound to a matrix suitable for disulfide exchange reactions and is removed from the matrix by reaction with a reducing agent. The method according to the invention makes it possible to provide the biologically active a subunit of factor XIII in high purity.

摘要翻译： 本发明涉及通过亲和层析纯化因子XIII亚单位的方法，涉及含有后者的治疗组合物和治疗组合物的用途。 因子XIII迄今为止通过技术上非常精细的方法或通过使用毒性亲和层析材料的方法进行纯化。 本发明的目的是提供一种改进因子XIII亚基的纯化方法。 因子XIII通过其中因子XIII的亚单位可逆地结合到适于二硫化物交换反应的基质的方法获得，并且通过与还原剂反应从基质中除去。 根据本发明的方法使得可以提供高纯度的因子XIII的生物活性的亚基。

公开(公告)号：US5097019A

公开(公告)日：1992-03-17

申请号：US649552

申请日：1991-02-01

申请人： Hartmut Lobermann ,  Christiane Bornmann

发明人： Hartmut Lobermann ,  Christiane Bornmann

IPC分类号： A61K38/00 ,  A61L2/00 ,  A61P7/02 ,  A61P7/04 ,  C07K14/47

CPC分类号： A61L2/0023 ,  C07K14/4721 ,  A61K38/00

摘要： An agent for the therapy or prophylaxis of disturbances of hemostasis, which contains tissue protein PP4, a process for the purification of PP4 by means of a hydrophobic adsorbent which is insoluble in water, and a process for the pasteurization of a solution containing PP4 in the presence of calcium ions and of at least one mono- or oligosaccharide or sugar alcohol and, where appropriate, of an amino acid, and the use of PP4, are described.

摘要翻译： 用于治疗或预防止血障碍的药剂，其含有组织蛋白质PP4，通过不溶于水的疏水性吸附剂纯化PP4的方法，以及在含有PP4的溶液中巴氏灭菌的方法 描述了钙离子和至少一种单糖或寡糖或糖醇的存在，以及在适当情况下存在的氨基酸和PP4的用途。

公开(公告)号：US4990597A

公开(公告)日：1991-02-05

申请号：US222998

申请日：1988-07-22

申请人： Hartmut Lobermann

发明人： Hartmut Lobermann

IPC分类号： C07K1/22 ,  C07K14/00 ,  C07K14/47

CPC分类号： C07K14/4721 ,  Y10S530/851

摘要： A process for the purification of tissue protein PP4 which can be obtained from placenta is described.This process makes use of the property of binding to saccharide polysulfates or to sulfated sugars in the presence of calcium ions.

摘要翻译： 描述了可以从胎盘获得的组织蛋白质PP4的纯化方法。 该方法利用在钙离子存在下结合糖多糖或硫酸化糖的性质。

公开(公告)号：US5049545A

公开(公告)日：1991-09-17

申请号：US323156

申请日：1989-03-15

申请人： Hartmut Lobermann ,  Eric P. Paques ,  Norbert Heimburger

发明人： Hartmut Lobermann ,  Eric P. Paques ,  Norbert Heimburger

IPC分类号： C07K14/575 ,  A61K38/00 ,  A61K38/28 ,  A61P3/08 ,  C07K1/06 ,  C07K1/113 ,  C07K14/62 ,  C07K14/76 ,  C07K14/81 ,  C07K19/00

CPC分类号： C07K14/62 ,  A61K38/00

摘要： Insulin derivatives of the general formulae I and IIX--(Y.sub.1 --S--S--Y.sub.2 --Z--"Insulin").sub.m IA--S--Y.sub.3 --Z--"Insulin" IIin whichX is a radical of a physiologically tolerated carbon compound,Y.sub.1, Y.sub.2 and Y.sub.3 are, independently of one another, a chemical bond or an organic chemical bridge,S is a sulfur atom,"Insulin" denotes the biologically active peptidic portion of a natural, semisynthetic or synthetic insulin or an insulin which has been prepared by genetic engineering, or one of its biologically active analogs without the inessential amino group,Z is an amino group which is inessential for the biological activity of insulin,A is a hydrogen atom or the radical of a mercapto-containing, physiologically tolerated hydrocarbon compound, andm is an integer from 1 to 20,agents containing these derivatives, a process for the preparation of these derivatives, and their use for the treatment of metabolic disorders, are described.

摘要翻译： 通式I和II的胰岛素衍生物X-（Y1-SS-Y2-Z-“胰岛素”）m I AS-Y3-Z-“胰岛素”II，其中X是生理耐受性碳化合物的基团，Y1 ，Y2和Y3彼此独立地是化学键或有机化学桥，S是硫原子，“胰岛素”表示已经制备的天然的，半合成的或合成的胰岛素或胰岛素的生物活性肽部分 通过遗传工程，或其生物活性类似物之一，其中没有非必需氨基，Z是对胰岛素生物活性无关的氨基，A是氢原子或含巯基的生理耐受性烃化合物 ，m为1〜20的整数，含有这些衍生物的试剂，这些衍生物的制备方法及其用于治疗代谢紊乱的用途。