利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.01 秒)

    Protease for activating clotting factor VII
    1.
    发明申请
    Protease for activating clotting factor VII 有权
    用于激活凝血因子VII的蛋白酶

    公开(公告)号：US20080175878A1

    公开(公告)日：2008-07-24

    申请号：US11984058

    申请日：2007-11-13

    申请人： Jurgen Romisch ,  Annette Feussner ,  Hans-Arnold Stohr

    发明人： Jurgen Romisch ,  Annette Feussner ,  Hans-Arnold Stohr

    IPC分类号： A61K9/00 ,  C12Q1/56 ,  A61P7/00 ,  A61K38/48

    CPC分类号： A61L15/38 ,  A61K38/00 ,  A61K38/57 ,  C07K14/745 ,  C12N9/6424 ,  C12Q1/56 ,  G01N33/86 ,  G01N2333/96447

    摘要： A protease for activating the blood clotting factor VII is described, which (a) is inhibited by the presence of aprotinin, (b) is increased in its activity by calcium ions and/or heparin or heparin-related substances, and (c) in SDS-PAGE, on subsequent staining in the non-reduced state, comprises one or more bands in the molecular weight range from 50 to 75 kDa; and in SDS-PAGE, on subsequent staining in the reduced state, comprises a band at 40 to 55 kDa, one or more bands in the molecular weight range from 10 to 35 kDa, and a band, which corresponds to a proenzyme, in the molecular weight range between 60 and 65 kDa.

    摘要翻译： 描述了用于激活凝血因子VII的蛋白酶，其中（a）被抑肽酶抑制，（b）钙离子和/或肝素或肝素相关物质的活性增加，（c） 在随后在非还原状态下染色的SDS-PAGE包含分子量范围为50至75kDa的一个或多个条带; 并且在SDS-PAGE中，在还原状态下的随后染色中，包含在40至55kDa的条带，分子量范围为10至35kDa的一个或多个条带和对应于酶原的条带 分子量范围在60和65kDa之间。

    Method for the purification of factor XIII by affinity chromatography
    2.
    发明授权
    Method for the purification of factor XIII by affinity chromatography 失效
    通过亲和层析纯化因子XIII的方法

    公开(公告)号：US5047506A

    公开(公告)日：1991-09-10

    申请号：US321133

    申请日：1989-03-09

    申请人： Hartmut Lobermann ,  Jurgen Romisch ,  Werner Stuber

    发明人： Hartmut Lobermann ,  Jurgen Romisch ,  Werner Stuber

    IPC分类号： A61K38/43 ,  A61K38/00 ,  A61P7/06 ,  C07K1/113 ,  C07K1/22 ,  C07K14/435 ,  C07K14/745 ,  C07K14/755 ,  C12N9/10

    CPC分类号： C12N9/1044 ,  A61K38/00

    摘要： The invention relates to a method for the purification of the a subunit of factor XIII by affinity chromatography, to a therapeutic composition containing the latter, and to the use of the therapeutic composition.Factor XIII has hitherto been purified either by methods which are technically very elaborate or else by use of toxic affinity chromatography materials. The invention has the aim of providing an improved method for the purification of the a subunit of factor XIII.Factor XIII is obtained according to the invention by a method in which the a subunit of factor XIII is reversibly bound to a matrix suitable for disulfide exchange reactions and is removed from the matrix by reaction with a reducing agent. The method according to the invention makes it possible to provide the biologically active a subunit of factor XIII in high purity.

    摘要翻译： 本发明涉及通过亲和层析纯化因子XIII亚单位的方法，涉及含有后者的治疗组合物和治疗组合物的用途。 因子XIII迄今为止通过技术上非常精细的方法或通过使用毒性亲和层析材料的方法进行纯化。 本发明的目的是提供一种改进因子XIII亚基的纯化方法。 因子XIII通过其中因子XIII的亚单位可逆地结合到适于二硫化物交换反应的基质的方法获得，并且通过与还原剂反应从基质中除去。 根据本发明的方法使得可以提供高纯度的因子XIII的生物活性的亚基。

    Protease for activating clotting factor VII
    3.
    发明申请
    Protease for activating clotting factor VII 审中-公开
    用于激活凝血因子VII的蛋白酶

    公开(公告)号：US20050202002A1

    公开(公告)日：2005-09-15

    申请号：US11118396

    申请日：2005-05-02

    申请人： Jurgen Romisch ,  Annette Feussner ,  Hans-Arnold Stohr

    发明人： Jurgen Romisch ,  Annette Feussner ,  Hans-Arnold Stohr

    IPC分类号： A61K38/46 ,  A61K38/00 ,  A61K38/48 ,  A61L15/38 ,  C07K14/745 ,  C12N9/64 ,  C12Q1/56 ,  G01N33/53 ,  G01N33/86

    CPC分类号： A61L15/38 ,  A61K38/00 ,  A61K38/57 ,  C07K14/745 ,  C12N9/6424 ,  C12Q1/56 ,  G01N33/86 ,  G01N2333/96447

    摘要： A protease for activating the blood clotting factor VII is described, which (a) is inhibited by the presence of aprotinin, (b) is increased in its activity by calcium ions and/or heparin or heparin-related substances, and (c) in SDS-PAGE, on subsequent staining in the non-reduced state, has one or more brands in the molecular weight range from 50 to 75 kDa and kDa in the reduced state has a band at 40 to 55 kDa and one or more bands in the molecular weight range from 10 to 35 kDa. The proenzyme of this protease is also characterized. Further, a process for obtaining this protease and its use in hemorrhage prophylaxis or hemostasis is described. Moreover, a test system for the qualitative and quantitative detection of a protease which activates the blood clotting factor VII is described.

    摘要翻译： 描述了用于激活凝血因子VII的蛋白酶，其中（a）被抑肽酶抑制，（b）钙离子和/或肝素或肝素相关物质的活性增加，（c） 在未还原状态下随后染色的SDS-PAGE在50至75kDa的分子量范围内具有一个或多个品牌，还原状态下的kDa具有40至55kDa的条带和 分子量范围为10至35kDa。 该蛋白酶的酶还具有特征。 此外，描述了获得该蛋白酶的方法及其在出血预防或止血中的用途。 此外，描述了用于定性和定量检测激活凝血因子VII的蛋白酶的测试系统。

    Method of providing a purified, virus safe antibody preparation
    6.
    发明申请
    Method of providing a purified, virus safe antibody preparation 有权
    提供纯化的病毒安全抗体制剂的方法

    公开(公告)号：US20070173638A1

    公开(公告)日：2007-07-26

    申请号：US10590900

    申请日：2005-02-25

    申请人： Andrea Buchacher ,  Gunther Iberer ,  Jurgen Romisch

    发明人： Andrea Buchacher ,  Gunther Iberer ,  Jurgen Romisch

    IPC分类号： C07K16/18

    CPC分类号： C07K16/065 ,  A61L2/0011 ,  A61L2/0017 ,  A61L2/0023

    摘要： A method of preparing a purified, virus inactivated and virus safe antibody preparation from a starting solution comprising antibodies and contaminants, the method comprising the steps of: (a) adjusting the pH of the starting solution to about 4.6 to about 4.95 in particular to about 4.8 to about 4.95 to produce an intermediate solution; (b) adding caprylate and/or heptanoate ions to the intermediate solution and maintaining the pH at about 4.6 to about 4.95 in particular pH at about 4.8 to about 4.95, whereby a precipitate is formed and the antibodies are essentially present in the supernatant; (c) incubating the supernatant solution under conditions of caprylate and/or heptanoate ion concentration, time, pH and temperature optionally concentrating and diafiltrating the filtrated solution before pH adjustment; (d) applying the filtered solution with a least one anion exchange resin and optionally with two different anion exchange resins under conditions that allow binding of contaminants to the resin while not allowing significant binding' of the antibodies to the resin, wherein a purified, virus inactivated and virus safe antibody preparation is produced.

    摘要翻译： 从包含抗体和污染物的起始溶液制备纯化的病毒灭活和病毒安全的抗体制剂的方法，所述方法包括以下步骤：（a）将起始溶液的pH调节至约4.6至约4.95，特别是约 4.8至约4.95以产生中间溶液; （b）向中间体溶液中加入辛酸酯和/或庚酸酯离子，并将pH保持在约4.6至约4.95，特别是约4.8至约4.95的pH，由此形成沉淀物，并且抗体基本上存在于上清液中; （c）在辛酸盐和/或庚酸盐离子浓度，时间，pH和温度条件下将上清液温育，任选地浓缩并在pH调节之前渗滤滤液; （d）在允许污染物与树脂结合的条件下使用至少一种阴离子交换树脂和任选地含有两种不同的阴离子交换树脂的过滤溶液，同时不允许抗体与树脂的显着结合，其中纯化的病毒 产生灭活和病毒安全的抗体制备。

    Universally applicable virus inactivated blood plasma produced from portions of non-caucasian plasma
    7.
    发明申请
    Universally applicable virus inactivated blood plasma produced from portions of non-caucasian plasma 审中-公开
    普遍适用的病毒灭活血浆是由非白血病部分血浆产生的

    公开(公告)号：US20070071829A1

    公开(公告)日：2007-03-29

    申请号：US10580548

    申请日：2004-12-20

    申请人： Andrea Heger ,  Jurgen Romisch ,  Tor-Einar Svae ,  Wolfgang Marguerre

    发明人： Andrea Heger ,  Jurgen Romisch ,  Tor-Einar Svae ,  Wolfgang Marguerre

    IPC分类号： A61K35/16 ,  C12N5/08

    CPC分类号： A61K35/16

    摘要： A blood plasma for human use pooled from donors which belong to 10% or more to a non-Caucasian population, the plasma obtainable by mixing blood or blood plasma of blood groups A and B, optionally AB without admixing substantial amounts of blood or blood plasma of blood group 0 characterized in that four to eight parts of blood or blood plasma from donors having the blood group A, more than three parts to seven parts of blood or blood plasma from donors having the blood group B, zero to two parts of blood or blood plasma from donors having the blood group AB.

    摘要翻译： 属于非高加索人口10％以上的供体的人血浆是通过混合血型A和B的血液或血浆而得到的血浆，任选地AB，而不混合大量的血液或血浆 的血型0，其特征在于，具有血型A，来自具有血型B的供体的血液或血浆中的血液或血浆的血液或血浆的血液或血浆中的血液或血浆的血液或血浆为4至8份，血液为零至2份 或具有血型AB的供体的血浆。

    Process for preparing an alpha-1-antitrypsin solution
    8.
    发明申请
    Process for preparing an alpha-1-antitrypsin solution 审中-公开
    制备α-1-抗胰蛋白酶溶液的方法

    公开(公告)号：US20060194300A1

    公开(公告)日：2006-08-31

    申请号：US10566354

    申请日：2004-08-12

    申请人： Petra Schulz ,  Jurgen Romisch

    发明人： Petra Schulz ,  Jurgen Romisch

    IPC分类号： C12N9/99

    CPC分类号： A61L2/0088 ,  A61L2/0017 ,  A61L2/0023 ,  C07K14/8125

    摘要： A process for preparing A1AT from A1AT-containing solutions, comprising the following steps: (a) subjecting an A1AT-containing solution to ion-exchange chromatography; (b) adding detergents and optionally a solvent for inactivating lipid-enveloped viruses; (c) followed by increasing the salt concentration to salt out the detergents. A1AT having a purity of >90% with an activity of ≧0.8 PEU/mg in its active form.

    摘要翻译： 一种从含有A1AT的溶液制备A1AT的方法，包括以下步骤：（a）使含A1AT的溶液进行离子交换层析; （b）添加去污剂和任选的用于灭活脂质包膜病毒的溶剂; （c），然后增加盐浓度以除去洗涤剂。 纯度为> 90％，活性为> = 0.8 PEU / mg的活性形式的A1AT。