公开(公告)号：US6007987A

公开(公告)日：1999-12-28

申请号：US730185

申请日：1996-10-15

Applicant: Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

Inventor： Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

IPC: C12Q1/68 ,  C40B40/06

CPC classification number: C12Q1/6837 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Abstract translation: 本发明涉及可用于使用包括探针，探针阵列和方法的杂交技术测​​序的用于测序核酸靶的方法和试剂，从而在离散包装中快速有效地获得序列信息。 该信息可以用于特定靶核酸的检测，鉴定，纯化和完全或部分测序。 当与连接步骤相结合时，这些方法可以在单一杂交条件下进行。 本发明还涉及探针阵列的复制和用于制备和复制探针阵列的方法，所述探针阵列可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

公开(公告)号：US5795714A

公开(公告)日：1998-08-18

申请号：US110691

申请日：1993-08-23

Applicant: Charles R. Cantor ,  Marek Przetakiewicz ,  Cassandra L. Smith ,  Takeshi Sano

Inventor： Charles R. Cantor ,  Marek Przetakiewicz ,  Cassandra L. Smith ,  Takeshi Sano

IPC: G01N33/53 ,  B01J19/00 ,  C07H21/04 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N37/00

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6837 ,  C40B80/00 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Abstract translation: 本发明涉及用于复制探针阵列的探针阵列的复制和可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物的方法。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。