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    Positional sequencing by hybridization
    2.
    发明授权
    Positional sequencing by hybridization 失效
    通过杂交进行位置测序

    公开(公告)号：US6007987A

    公开(公告)日：1999-12-28

    申请号：US730185

    申请日：1996-10-15

    申请人： Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

    发明人： Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

    IPC分类号： C12Q1/68 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

    摘要： This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

    摘要翻译： 本发明涉及可用于使用包括探针，探针阵列和方法的杂交技术测​​序的用于测序核酸靶的方法和试剂，从而在离散包装中快速有效地获得序列信息。 该信息可以用于特定靶核酸的检测，鉴定，纯化和完全或部分测序。 当与连接步骤相结合时，这些方法可以在单一杂交条件下进行。 本发明还涉及探针阵列的复制和用于制备和复制探针阵列的方法，所述探针阵列可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

    Nucleic acid directed immobilization arrays and methods of assembly
    6.
    发明授权
    Nucleic acid directed immobilization arrays and methods of assembly 失效
    核酸定向固定阵列和组装方法

    公开(公告)号：US07569341B2

    公开(公告)日：2009-08-04

    申请号：US08967269

    申请日：1997-11-07

    申请人： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    发明人： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    IPC分类号： C12Q1/68

    CPC分类号： B82Y5/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/557 ,  A61K47/6949 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682

    摘要： The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurality of bioreactive complexes The plurality of bioreactive complexes are hybridized to the plurality of first nucleic acids to form the supramolecular bioconjugate. Bioconjugates can be used to detect and isolate targets, to screen samples for targets such as antigens, to treat patients with multiple agents or to diagnose disorders in the form of a kit.

    摘要翻译： 本发明涉及超分子生物缀合物和组分和利用超分子生物缀合物的方法。 超分子生物缀合物包含多个第一核酸和多个介体，其中每个介体包含与所述多个第一核酸内的序列互补的第二核酸。 为了组装超分子生物缀合物，将一组或多组生物反应剂偶联到多个介体中，形成多个生物反应性复合物。多个生物反应性复合物与多个第一核酸杂交以形成超分子生物缀合物。 生物共轭物可用于检测和分离靶标，筛选靶标如抗原的样品，以治疗患有多种药物的患者或以试剂盒的形式诊断疾病。

    Biotin-binding containment systems
    8.
    发明授权
    Biotin-binding containment systems 失效
    生物素结合遏制系统

    公开(公告)号：US5681745A

    公开(公告)日：1997-10-28

    申请号：US432017

    申请日：1995-05-01

    申请人： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

    发明人： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

    IPC分类号： C07K14/36 ,  C07K14/465 ,  C12N1/21 ,  C12N15/12 ,  C12N15/70 ,  C12N5/10 ,  C12N1/13

    CPC分类号： C12N15/70 ,  C07K14/36 ,  C07K14/465

    摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

    摘要翻译： 本发明涉及表达可用于选择性破坏重组细胞如遗传工程微生物的生物素结合成分的遗传容纳系统。 这些系统可以包含链霉亲和素或抗生物素蛋白基因，其表达受可调节启动子控制。 诸如转录效应子的调节剂由另外可能被表达的基因表达，其表达由遏制系统控制。 试剂的表达可以设计成响应环境中的生理变化。 本发明还涉及用于选择性检测或跟踪重组细胞和含有这些遗传包涵体系的真核和原核细胞的遏制系统和方法。

    SUPRAMOLECULAR BIOCONJUGATES
    9.
    发明申请
    SUPRAMOLECULAR BIOCONJUGATES 审中-公开

    公开(公告)号：US20100255558A1

    公开(公告)日：2010-10-07

    申请号：US12435665

    申请日：2009-05-05

    申请人： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    发明人： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    IPC分类号： C07K17/00 ,  C07K1/00 ,  C12N9/96

    CPC分类号： B82Y5/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/557 ,  A61K47/6949 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682

    摘要： The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurality of bioreactive complexes. The plurality of bioreactive complexes are hybridized to the plurality of first nucleic acids to form the supramolecular bioconjugate. Bioconjugates can be used to detect and isolate targets, to screen samples for targets such as antigens, to treat patients with multiple agents or to diagnose disorders in the form of a kit.

    摘要翻译： 本发明涉及超分子生物缀合物和组分和利用超分子生物缀合物的方法。 超分子生物缀合物包含多个第一核酸和多个介体，其中每个介体包含与所述多个第一核酸内的序列互补的第二核酸。 为了组装超分子生物缀合物，将一组或多组生物反应剂偶联至多个介体，形成多个生物反应性复合物。 多个生物反应性复合物与多个第一核酸杂交以形成超分子生物缀合物。 生物共轭物可用于检测和分离靶标，筛选靶标如抗原的样品，以治疗患有多种药物的患者或以试剂盒的形式诊断疾病。