公开(公告)号：US20230352118A1

公开(公告)日：2023-11-02

申请号：US18311138

申请日：2023-05-02

Applicant: Merck Sharp and Dohme LLC

Inventor： Yara Seif ,  Xiao Xiao ,  Kevin Teng ,  Ming-Tang Chen ,  Alan C. Cheng ,  BoRam Lee ,  Isha Verma

IPC: G16B30/10 ,  G16B5/00

CPC classification number: G16B30/10 ,  G16B5/00

Abstract: A computer-implemented method is disclosed for candidate antibody exploration. The method includes receiving sequence reads from a sequencing system, wherein each sequence read comprises a target gene for expressing a candidate antibody, wherein each sequence read is associated with a binding affinity to a target antigen. The method includes generating a sequence representation for each sequence read, representing the amino acid sequences of the sequence read. The method includes training a binding affinity prediction model with the sequence representations and the binding affinities. The method includes generating a synthetic candidate sequence read that is different from the sequence reads. The method includes generating a sequence representation for the synthetic candidate sequence read. The method includes determining a binging affinity prediction for the synthetic candidate sequence read by applying the binding affinity prediction model to the sequence representation for the synthetic candidate sequence read.

公开(公告)号：US20250092089A1

公开(公告)日：2025-03-20

申请号：US18890289

申请日：2024-09-19

Applicant: North Carolina State University ,  Merck Sharp and Dohme LLC ,  EMD Millipore Corporation ,  Rensselaer Polytechnic Institute

Inventor： Stefano Menegatti ,  Ruben G. Carbonell ,  Jacob P. LeBarre ,  Cristiana Boi ,  Steven Cramer ,  Michael Phillips ,  David Roush

IPC: C07K1/16 ,  B01D15/34 ,  B01D15/36

Abstract: The present disclosure provides methods related to the purification of a biologic. In particular, the present disclosure methods for purifying a biologic using a multifunctional chromatography medium comprising porous beads or particles having a defined pore diameter distribution and one or more ligands conjugated to the surface of the pores capable of removing process-related impurities and product-related impurities from a target biologic.