公开(公告)号：US20140186841A1

公开(公告)日：2014-07-03

申请号：US14034153

申请日：2013-09-23

申请人： Frederic ZENHAUSERN ,  Ralf LENIGK ,  Jianing YANG ,  Zhi CAI ,  Alan NORDQUIST ,  Stanley D. SMITH ,  David MAGGIANO ,  Glen MCCARTY ,  Mrinalini PRASAD ,  Karem LINAN ,  Baiju THOMAS ,  Edward OLAYA ,  Cedric HURTH ,  Darryl COX ,  Mark RICHARD

发明人： Frederic ZENHAUSERN ,  Ralf LENIGK ,  Jianing YANG ,  Zhi CAI ,  Alan NORDQUIST ,  Stanley D. SMITH ,  David MAGGIANO ,  Glen MCCARTY ,  Mrinalini PRASAD ,  Karem LINAN ,  Baiju THOMAS ,  Edward OLAYA ,  Cedric HURTH ,  Darryl COX ,  Mark RICHARD

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0672 ,  B01L2400/0677 ,  G01N2035/00158

摘要： A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction.

摘要翻译： 用于分析生物样品的方法，系统和装置包括接收样品，其中样品包括脱氧核糖核酸（DNA），裂解样品以获得样品中包含的DNA，纯化样品中的DNA以分离 来自样品中的其他成分的DNA，扩增DNA，分离扩增的DNA片段，使用激光诱导的荧光检测分离的片段，基于检测，产生所接收的样品中DNA的谱，比较生成的分布与 存储在数据库中的DNA的简档，以及在确定生成的简档与所存储的简档中的一个匹配时，识别从其获得存储的简档的源，其中对相应部分执行接收，裂解，净化，放大和检测 的微流体装置，并且其中将样品和DNA运输到微流体装置的部分并且使得能够进行裂解， 净化，放大，分离，检测，生成，比较和识别是自动执行的，无需用户交互。

公开(公告)号：US20120082985A1

公开(公告)日：2012-04-05

申请号：US12672889

申请日：2008-08-11

申请人： Frederic Zenhausern ,  Ralf Lenigk ,  Jianing Yang ,  Zhi Cai ,  Alan Nordquist ,  Stanley D. Smith ,  David Maggiano ,  Mrinalini Prasad ,  Karem Linan ,  Edward Olaya ,  Baiju Thomas ,  Cedric Hurth ,  Darryl Cox ,  Mark Richard ,  Glen Mccarty

发明人： Frederic Zenhausern ,  Ralf Lenigk ,  Jianing Yang ,  Zhi Cai ,  Alan Nordquist ,  Stanley D. Smith ,  David Maggiano ,  Mrinalini Prasad ,  Karem Linan ,  Edward Olaya ,  Baiju Thomas ,  Cedric Hurth ,  Darryl Cox ,  Mark Richard ,  Glen Mccarty

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N27/447 ,  C12M1/34 ,  C12M1/40

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0672 ,  B01L2400/0677 ,  G01N2035/00158

摘要： A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction.

摘要翻译： 用于分析生物样品的方法，系统和装置包括接收样品，其中样品包括脱氧核糖核酸（DNA），裂解样品以获得样品中包含的DNA，纯化样品中的DNA以分离 来自样品中的其他成分的DNA，扩增DNA，分离扩增的DNA片段，使用激光诱导的荧光检测分离的片段，基于检测，产生所接收的样品中DNA的谱，比较生成的分布与 存储在数据库中的DNA的简档，以及在确定生成的简档与所存储的简档中的一个匹配时，识别从其获得存储的简档的源，其中对相应部分执行接收，裂解，净化，放大和检测 的微流体装置，并且其中将样品和DNA运输到微流体装置的部分并且使得能够进行裂解， 净化，放大，分离，检测，生成，比较和识别是自动执行的，无需用户交互。