公开(公告)号：US20240010674A1

公开(公告)日：2024-01-11

申请号：US18251412

申请日：2021-11-08

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Kristopher E. Richardson ,  Daniel J. O'Neal ,  Jerald K. Rasmussen ,  Andrew W. Vail ,  Alexei M. Voloshin

IPC: C07K1/32 ,  C07K1/34 ,  C07K1/36 ,  C07K16/06 ,  B01D39/16 ,  B01D71/56 ,  B01D37/00

CPC classification number: C07K1/32 ,  C07K1/34 ,  C07K1/36 ,  C07K16/065 ,  B01D39/1623 ,  B01D71/56 ,  B01D37/00 ,  B01D2239/0618 ,  B01D2239/0622 ,  B01D2239/1233 ,  B01D2239/1266 ,  B01D2239/1216

Abstract: Described herein is a method of purifying a target molecule from an aqueous biological composition, the method comprising: (a) contacting a cationic polymer and the aqueous biological composition to form a mixture, the mixture comprising a bio-polymer complex and the target non-binding molecule in a liquid, wherein the bio-polymer complex has an average particle diameter of at least 50 micrometers; (b) providing a filtering unit comprising (i) a housing having an inlet and an outlet, (ii) a porous, continuous filter medium which is fluidly connected to the inlet and the outlet, and (iii) a collection region upstream from the porous, continuous filter medium; (c) adding the mixture to the inlet; and (d) allowing the mixture to separate in the filtering unit, whereby the bio-polymer complex collects in the collection region and the target non-binding molecule passes through the filter medium, and wherein the majority of flow of the liquid through the porous, continuous filter medium is not substantially parallel with the direction of gravity.

公开(公告)号：US20240059731A1

公开(公告)日：2024-02-22

申请号：US17766356

申请日：2020-11-24

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Kristopher E. Richardson ,  Daniel J. O'Neal ,  Jerald K. Rasmussen ,  Andrew W. Vail

IPC: C07K1/32 ,  C07K1/34

CPC classification number: C07K1/32 ,  C07K1/34

Abstract: Described herein is a method of purifying a target molecule from an aqueous biological composition, the method comprising: (a) contacting a cationic polymer and the aqueous biological composition to form a mixture comprising a bio-polymer complex and the target molecule in a liquid, wherein the bio-polymer complex has an average particle diameter of at least 45 micrometers, (b) adding the mixture to a filtering volume of a vessel, wherein the vessel comprises loosely packed staple fibers; (c) allowing the mixture to separate through the loosely packed staple fiber; and (d) collecting a filtrate comprising the target molecule.