公开(公告)号：US20240010674A1

公开(公告)日：2024-01-11

申请号：US18251412

申请日：2021-11-08

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Kristopher E. Richardson ,  Daniel J. O'Neal ,  Jerald K. Rasmussen ,  Andrew W. Vail ,  Alexei M. Voloshin

IPC: C07K1/32 ,  C07K1/34 ,  C07K1/36 ,  C07K16/06 ,  B01D39/16 ,  B01D71/56 ,  B01D37/00

CPC classification number: C07K1/32 ,  C07K1/34 ,  C07K1/36 ,  C07K16/065 ,  B01D39/1623 ,  B01D71/56 ,  B01D37/00 ,  B01D2239/0618 ,  B01D2239/0622 ,  B01D2239/1233 ,  B01D2239/1266 ,  B01D2239/1216

Abstract: Described herein is a method of purifying a target molecule from an aqueous biological composition, the method comprising: (a) contacting a cationic polymer and the aqueous biological composition to form a mixture, the mixture comprising a bio-polymer complex and the target non-binding molecule in a liquid, wherein the bio-polymer complex has an average particle diameter of at least 50 micrometers; (b) providing a filtering unit comprising (i) a housing having an inlet and an outlet, (ii) a porous, continuous filter medium which is fluidly connected to the inlet and the outlet, and (iii) a collection region upstream from the porous, continuous filter medium; (c) adding the mixture to the inlet; and (d) allowing the mixture to separate in the filtering unit, whereby the bio-polymer complex collects in the collection region and the target non-binding molecule passes through the filter medium, and wherein the majority of flow of the liquid through the porous, continuous filter medium is not substantially parallel with the direction of gravity.

公开(公告)号：US20210396725A1

公开(公告)日：2021-12-23

申请号：US17288031

申请日：2019-12-16

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Jonathan F. Hester ,  Eric J. Olson ,  Francis E. Porbeni ,  Andrew W. Vail ,  Alexei M. Voloshin

IPC: G01N30/96 ,  C07K1/22

Abstract: Described herein is method for testing an ion exchange chromatography device. The method includes monitoring both a binding and a non-binding species and determining their breakthrough point to determine a net breakthrough value. The method can be used to determine the integrity of the chromatography device, ensure that the chromatography device possesses the expected adsorbent capacity, and/or determine viral clearance of the chromatography device.