公开(公告)号：US20230140486A1

公开(公告)日：2023-05-04

申请号：US17913473

申请日：2021-04-14

Applicant: Academia Sinica

Inventor： Shih-Hsiung WU ,  I-Ming LEE

IPC: C07K14/005 ,  C12Q1/04

Abstract: A fusion protein including a phage tail-spike protein ΦAB6TSP and a signal indicator is provided. Also, a method of detecting bacteria having pseudaminic acid (Pse) is provided, including steps of contacting a sample with a phage tail-spike protein ΦDAB6TSP; and detecting a signal from the sample. The fusion protein and the method of detecting bacteria can be applied to a set of practical diagnosis and therapeutic alternative against Pse-coated antibiotic resistant pathogenic bacteria.

公开(公告)号：US20230102930A1

公开(公告)日：2023-03-30

申请号：US17955546

申请日：2022-09-29

Applicant: Academia Sinica ,  I-MEI FOODS CO., LTD.

Inventor： Shih-Hsiung WU ,  Ya-Chu LIEN ,  Chi-Ming KAO ,  Ken-Pei WONG

IPC: C12P21/06 ,  C12N1/12

Abstract: Disclosed herein are novel methods for hydrolyzing eggshell membrane (ESM). In one embodiment, the method includes cultivating thermophilic bacteria in a solution containing 1-10% (wt %) ESM to decompose the ESM into the ESM hydrolysate; wherein, the thermophilic bacteria grow on the ESM as their sole source of nutrient. In another embodiment, the method includes treating ESM with a keratinase in the presence of a reducing agent at a condition sufficient to produce the ESM hydrolysate, in which the keratinase, the reducing agent, and the ESM are present in a weight ratio of 1:120:600. The thus produced ESM hydrolysate is enriched in essential amino acids, collagen, peptides and glycosaminoglycans.

公开(公告)号：US20210123927A1

公开(公告)日：2021-04-29

申请号：US17084125

申请日：2020-10-29

Applicant: National Defense Medical Center ,  Academia Sinica

Inventor： Ann CHEN ,  Shuk-Man KA ,  Shih-Hsiung WU

IPC: G01N33/68

Abstract: The present invention provides a lectin from Crenomytilus grayanus or Mytilus trossulus as diagnostic reagents for IgA nephropathy. The present invention also provides a diagnostic kit for detecting Galactose-deficient IgA1 (Gd-IgA1), comprising RussiaSea-001 (also called as CGL) isolated from Crenomytilus grayanus or RussiaSea-002 (also called as MTL) isolated from Mytilus trossulus. The present invention further provides a method for detecting Gd-IgA1 using RussiaSea-001 or RussiaSea-002

公开(公告)号：US20160145593A1

公开(公告)日：2016-05-26

申请号：US14903194

申请日：2014-07-17

Applicant: ACADEMIA SINICA

Inventor： Meng-Chiao HO ,  Shih-Hsiung WU ,  Wan-Ling WU

IPC: C12N9/52 ,  C12P21/06 ,  C07K14/39 ,  C12N15/52

CPC classification number: C12N9/52 ,  C07K14/39 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/50 ,  C12N15/52 ,  C12P21/06 ,  C12Y304/21

Abstract: A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.

Abstract translation: 公开了一种编码台湾华ensis WR-220角蛋白酶的融合基因。 融合物包含：（a）编码位于融合基因N末端的蛋白质分泌信号肽的第一DNA序列; （b）编码与第一DNA序列连接的翻译框架的台湾华ensis WR-220角蛋白酶的抑制结构域的第二DNA序列; 和（c）编码与第二DNA序列连接的翻译框架的台湾华南WR-220角蛋白酶的催化结构域的第三DNA序列，其中融合基因是非天然存在的嵌合DNA。 还公开了一种制备M. taiwanensis WR-220角蛋白酶的催化结构域的方法，并使用了台湾华氏WR-220角蛋白酶。

公开(公告)号：US20160297829A1

公开(公告)日：2016-10-13

申请号：US14681553

申请日：2015-04-08

Applicant: Academia Sinica

Inventor： Shih-Hsiung WU ,  Ching-Ming CHANG

IPC: C07D493/08 ,  C07D307/33 ,  A61K45/06 ,  A61K31/365

CPC classification number: C07D493/08 ,  A61K31/365 ,  A61K36/06 ,  A61K45/06 ,  C07D307/33 ,  Y02A50/469 ,  Y02A50/473 ,  A61K2300/00

Abstract: Disclosed herein are novel uses of avenaciolide derivatives and the preparation method of producing the same. The avenaciolide derivatives may suppress or inhibit the growth of gram-positive bacteria, including the notorious methicillin-resistant Staphylococcus aureus. Accordingly, the avenaciolides derivatives are potential lead compounds for the development of next generation antibiotics for the treatment of disease and/or disorders related to infection caused by gram-positive bacteria.

Abstract translation: 本文公开了一种新戊烯衍生物的新用途及其制备方法。 阿维菌素衍生物可抑制或抑制革兰氏阳性菌的生长，包括臭名昭着的耐甲氧西林金黄色葡萄球菌。 因此，阿维菌素衍生物是用于开发用于治疗与由革兰氏阳性菌引起的感染相关的疾病和/或病症的下一代抗生素的潜在的铅化合物。