公开(公告)号：US20150184259A1

公开(公告)日：2015-07-02

申请号：US14583566

申请日：2014-12-26

Applicant: API Intellectual Property Holdings, LLC

Inventor： Ryan ZEBROSKI ,  James SCHULTZE

IPC: C13K1/02 ,  C12P19/02 ,  C13K1/04 ,  C12P7/10 ,  C12P19/14

CPC classification number: C13K1/02 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/04 ,  Y02E50/16

Abstract: The present invention is capable of producing glucose and hemicellulose sugars from lignocellulosic biomass. In some variations, a process includes optionally pre-steaming a biomass feedstock; extracting the feedstock with liquid hot water to produce glucan-rich solids and an extract liquor containing dissolved solids, which include hemicellulosic oligomers and lignin; washing the glucan-rich solids; hydrolyzing the hemicellulosic oligomers by contacting the extract liquor with an acid catalyst or enzymes possessing hemicellulase activity; separately hydrolyzing the glucan by contacting the glucan-rich solids stream with an acid catalyst or enzymes possessing glucanase activity, optionally with removing the glucose in situ by microfiltration and/or ultrafiltration; and recovering or fermenting each of the hemicellulosic monomers and the glucose. Preferred configurations and conditions are disclosed.

Abstract translation: 本发明能够从木质纤维素生物质生产葡萄糖和半纤维素糖。 在一些变型中，方法包括任选地预蒸汽生物质原料; 用液体热水提取原料以产生富含葡聚糖的固体，以及含有溶解的固体的提取液，包括半纤维素低聚物和木质素; 洗涤富含葡聚糖的固体; 通过使萃取液与酸催化剂或具有半纤维素酶活性的酶接触来水解半纤维素低聚物; 通过使富含葡聚糖的固体物流与酸催化剂或具有葡聚糖酶活性的酶接触来分别水解葡聚糖，任选地通过微量过滤和/或超滤原位除去葡萄糖; 并回收或发酵每种半纤维素单体和葡萄糖。 公开了优选的配置和条件。

公开(公告)号：US20150275252A1

公开(公告)日：2015-10-01

申请号：US14670457

申请日：2015-03-27

Applicant: API Intellectual Property Holdings, LLC

Inventor： Theodora RETSINA ,  Vesa PYLKKANEN ,  Ryan ZEBROSKI

IPC: C12P19/04 ,  C12P7/56 ,  C12P7/04 ,  C12P7/54 ,  C12P19/02 ,  C12P7/10

CPC classification number: C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  Y02E50/16

Abstract: In some variations, this invention provides a process for producing fermentable sugars from cellulosic biomass, comprising: extracting biomass with steam or hot water to produce an extract liquor containing hemicellulose oligomers, dissolved lignin, and cellulose-rich solids; separating and washing the cellulose-rich solids; removing a portion of glucan contained in the washed cellulose-rich solids as glucose oligomers using a liquefaction-focused blend of enzymes; co-hydrolyzing glucose oligomers and hemicellulose oligomers, with enzymes or an acid catalyst, to produce glucose and hemicellulose monomers; and recovering the glucose and hemicellulose monomers as fermentable sugars. The liquefaction-focused blend of enzymes contains endoglucanases and exoglucanases. A rotating unit for high-solids enzymatic hydrolysis may be employed, with continuous liquid removal. Optionally, the glucose and the hemicellulose monomers may be recovered as separate streams. The residual cellulose (not hydrolyzed) as well as the lignin may be recovered and combusted, or utilized for other purposes.

Abstract translation: 在一些变型中，本发明提供了一种从纤维素生物质生产可发酵糖的方法，其包括：用蒸汽或热水提取生物质以产生含有半纤维素低聚物，溶解的木质素和富含纤维素的固体的提取液; 分离和洗涤富含纤维素的固体; 使用液化聚焦的酶将所洗涤的富含纤维素的固体中含有的一部分葡聚糖作为葡萄糖寡聚物除去; 用酶或酸催化剂共同水解葡萄糖低聚物和半纤维素寡聚物以产生葡萄糖和半纤维素单体; 并将葡萄糖和半纤维素单体作为可发酵糖回收。 液化聚焦的混合物​​含有内切葡聚糖酶和外切葡聚糖酶。 可以使用用于高固体酶水解的旋转单元，其中连续除去液体。 任选地，葡萄糖和半纤维素单体可以作为单独的流回收。 残留的纤维素（未水解）以及木质素可以回收和燃烧，或用于其它目的。

公开(公告)号：US20150184260A1

公开(公告)日：2015-07-02

申请号：US14583572

申请日：2014-12-26

Applicant: API Intellectual Property Holdings, LLC

Inventor： Theodora RETSINA ,  Vesa PYLKKANEN ,  Ryan ZEBROSKI

IPC: C13K1/02 ,  C12P19/02 ,  C12P19/14 ,  C13K13/00

CPC classification number: C13K1/02 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K13/002 ,  Y02E50/16

Abstract: In some variations, this invention provides a process for producing fermentable sugars from cellulosic biomass, comprising: extracting biomass with steam or hot water to produce an extract liquor containing hemicellulose oligomers, dissolved lignin, and cellulose-rich solids; separating and washing the cellulose-rich solids; removing a portion of glucan contained in the washed cellulose-rich solids as glucose oligomers using a liquefaction-focused blend of enzymes; co-hydrolyzing glucose oligomers and hemicellulose oligomers, with enzymes or chemical catalyst, to produce glucose and hemicellulose monomers; and recovering the glucose and hemicellulose monomers as fermentable sugars. The liquefaction-focused blend of enzymes contains endoglucanases and exoglucanases. Optionally, the glucose and the hemicellulose monomers may be recovered as separate streams. The residual cellulose (not hydrolyzed) as well as the lignin may be recovered and combusted, or utilized for other purposes, such as for energy-dense pellets.

Abstract translation: 在一些变型中，本发明提供了一种从纤维素生物质生产可发酵糖的方法，其包括：用蒸汽或热水提取生物质以产生含有半纤维素低聚物，溶解的木质素和富含纤维素的固体的提取液; 分离和洗涤富含纤维素的固体; 使用液化聚焦的酶将所洗涤的富含纤维素的固体中含有的一部分葡聚糖作为葡萄糖寡聚物除去; 用酶或化学催化剂共同水解葡萄糖寡聚体和半纤维素寡聚物以产生葡萄糖和半纤维素单体; 并将葡萄糖和半纤维素单体作为可发酵糖回收。 液化聚焦的混合物​​含有内切葡聚糖酶和外切葡聚糖酶。 任选地，葡萄糖和半纤维素单体可以作为单独的流回收。 残留的纤维素（未水解）以及木质素可以回收和燃烧，或用于其它目的，例如用于能量密度的颗粒。

公开(公告)号：US20190177755A1

公开(公告)日：2019-06-13

申请号：US16029929

申请日：2018-07-09

Applicant: API Intellectual Property Holdings, LLC

Inventor： Theodora RETSINA ,  Ryan ZEBROSKI

IPC: C12P19/02 ,  C12P19/14

Abstract: A method of enzymatically hydrolyzing pretreated lignocellulosic biomass at high solids concentration includes introducing pretreated biomass to a hydrolysis reactor, to hydrolyze the cellulose to glucose monomer and glucose oligomers, and circulating a liquid stream, from which glucose is removed to reduce glucose inhibition of cellulose hydrolysis. A surfactant may be added to the hydrolysis reactor. Heat and/or acid treatment of the glucose oligomers may be used to generate additional glucose monomer. Some variations introduce pretreated biomass to a hydrolysis reactor to hydrolyze cellulose to glucose monomer and glucose oligomers, followed by conveying a portion of the solid phase to a mechanical refiner and/or a unit under reduced pressure, to generate a refined and/or exploded solid phase; and recycling the refined and/or exploded solid phase, optionally reheated, back to an input of the hydrolysis reactor.

公开(公告)号：US20150232580A1

公开(公告)日：2015-08-20

申请号：US14623853

申请日：2015-02-17

Applicant: API Intellectual Property Holdings, LLC

Inventor： Theodora RETSINA ,  Jean-Pierre MONCLIN ,  Ryan ZEBROSKI ,  Anastasios TRYPAKIS ,  Vesa PYLKKANEN

IPC: C08B15/00

CPC classification number: C08B15/00 ,  C08H8/00

Abstract: The disclosure provides a process for separating fermentation inhibitors from a biomass-derived hydrolysate, comprising: introducing a biomass-derived liquid hydrolysate stream to a stripping column; introducing a steam-rich vapor stream to the stripping column to strip fermentation inhibitors (such as acetic acid) from the liquid hydrolysate stream; recovering a stripped liquid stream and a stripper vapor output stream; compressing the stripper vapor output stream; introducing the compressed vapor stream, and a water-rich liquid stream, to an evaporator; recovering, from the evaporator, an evaporated liquid stream and an evaporator output vapor stream; and recycling the evaporator output vapor stream to the stripping column as the steam-rich vapor stream. Other variations utilize a rectification column to recover a rectified liquid stream and a rectification column vapor stream, and recycle the rectification column vapor stream to the stripping column as the steam-rich vapor stream.

Abstract translation: 本公开提供了从生物质衍生的水解产物中分离发酵抑制剂的方法，包括：将生物质衍生的液体水解产物流引入汽提塔; 将蒸汽蒸汽流引入汽提塔以从液体水解产物流中分离出发酵抑制剂（如乙酸）; 回收剥离的液体流和汽提蒸气输出流; 压缩汽提蒸汽输出流; 将压缩蒸汽流和富水液体流引入蒸发器; 从蒸发器中回收蒸发的液体流和蒸发器输出蒸气流; 并将蒸发器输出蒸气流循环到汽提塔作为富蒸气蒸气流。 其他变化使用精馏塔来回收精馏液流和精馏塔蒸气流，并将精馏塔蒸气流作为富蒸气流再循环至汽提塔。

公开(公告)号：US20170342444A1

公开(公告)日：2017-11-30

申请号：US15605930

申请日：2017-05-25

Applicant: API Intellectual Property Holdings, LLC

Inventor： Shrikant SURVASE ,  Ryan ZEBROSKI ,  Georgios ADAMOS ,  Vesa PYLKKANEN

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P19/02 ,  C12P7/04 ,  C12P7/52 ,  C12P7/54 ,  C12P19/14

CPC classification number: C12P7/10 ,  C12P7/04 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C5 and C6 sugars to fermentation products, the method comprising: fermenting a C6-rich sugar feed to a first fermentation product; fermenting a C5-rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C6-rich fermentor.

公开(公告)号：US20170204440A1

公开(公告)日：2017-07-20

申请号：US15276834

申请日：2016-09-27

Applicant: API Intellectual Property Holdings, LLC

Inventor： Theodora RETSINA ,  Ryan ZEBROSKI

IPC: C12P19/02

CPC classification number: C12P19/02 ,  C12P19/14 ,  C12P2203/00

Abstract: A method of enzymatically hydrolyzing pretreated lignocellulosic biomass at high solids concentration includes introducing pretreated biomass to a hydrolysis reactor, to hydrolyze the cellulose to glucose monomer and glucose oligomers, and circulating a liquid stream, from which glucose is removed to reduce glucose inhibition of cellulose hydrolysis. A surfactant may be added to the hydrolysis reactor. Heat and/or acid treatment of the glucose oligomers may be used to generate additional glucose monomer. Some variations introduce pretreated biomass to a hydrolysis reactor to hydrolyze cellulose to glucose monomer and glucose oligomers, followed by conveying a portion of the solid phase to a mechanical refiner and/or a unit under reduced pressure, to generate a refined and/or exploded solid phase; and recycling the refined and/or exploded solid phase, optionally reheated, back to an input of the hydrolysis reactor.