公开(公告)号：US20220372586A1

公开(公告)日：2022-11-24

申请号：US17879871

申请日：2022-08-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20240094229A1

公开(公告)日：2024-03-21

申请号：US18463628

申请日：2023-09-08

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Soma Chaudhary ,  Kirstie Swingle

IPC: G01N33/94 ,  C12Q1/6823 ,  G01N33/53 ,  G01N33/542 ,  G01N33/543

CPC classification number: G01N33/9446 ,  C12Q1/6823 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/54388

Abstract: Provided herein are highly modular and sensitive biosensors for small molecule detection using CRISPR/C as enzymes having collateral single-stranded nucleic acid cleavage activity. Also provided herein are uses of the biosensors in detection platforms for convenient, low-cost assessment of safety and purity of consumable samples, environmental samples, and agricultural products.

公开(公告)号：US11434540B2

公开(公告)日：2022-09-06

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6858 ,  C12Q1/6897

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20200071777A1

公开(公告)日：2020-03-05

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.