公开(公告)号：US20240052400A1

公开(公告)日：2024-02-15

申请号：US18453129

申请日：2023-08-21

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Duo Ma ,  Anli Tang

IPC: C12Q1/6825 ,  C12Q1/6816

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  Y02A50/30

Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.

公开(公告)号：US11773430B2

公开(公告)日：2023-10-03

申请号：US16342722

申请日：2017-10-17

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Duo Ma ,  Anli Tang

IPC: C12Q1/6825 ,  C12Q1/6816

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  Y02A50/30 ,  C12Q1/6825 ,  C12Q2525/205 ,  C12Q1/6816 ,  C12Q2525/205 ,  C12Q2565/625

Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.

公开(公告)号：US20220372586A1

公开(公告)日：2022-11-24

申请号：US17879871

申请日：2022-08-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US11434540B2

公开(公告)日：2022-09-06

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6858 ,  C12Q1/6897

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20200071777A1

公开(公告)日：2020-03-05

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US10550440B2

公开(公告)日：2020-02-04

申请号：US16079740

申请日：2017-02-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander A. Green ,  Anli Tang

IPC: C12Q1/6897 ,  C12N15/113 ,  C12N15/67 ,  C07K14/435

Abstract: Provided herein is a RNA-based riboregulator that detects protein translation and uses this stimulus to regulate expression of an unrelated protein. In one embodiment, provided herein is a synthetic nucleic acid molecule configured as a translation-sensing riboregulator, where the synthetic nucleic acid molecule comprises a first nucleotide sequence encoding a polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence.