公开(公告)号：US20240077422A1

公开(公告)日：2024-03-07

申请号：US18461044

申请日：2023-09-05

Applicant: Vincent Chabottaux ,  Thomas Glouden ,  Benoit Granier

Inventor： Vincent Chabottaux ,  Thomas Glouden ,  Benoit Granier

IPC: G01N21/64 ,  G01N21/25 ,  G01N33/543 ,  G01N33/58 ,  G01N33/94

CPC classification number: G01N21/6428 ,  G01N21/253 ,  G01N21/6452 ,  G01N21/6456 ,  G01N33/54388 ,  G01N33/582 ,  G01N33/9446 ,  G01N2021/6439 ,  G01N2021/7759 ,  G01N2201/0692

Abstract: Immuno-chromatographic diagnosis means (1) for detecting and/or quantifying a plurality of analytes present in an essentially liquid sample (E), comprising:



at least one reaction mixture (2) containing recognition biological molecules and/or competitive ligands labelled with at least one visualisation molecule which is detectable in fluorescence, said reaction mixture being present in a separate container of said recovery system (3); and
at least one recovery system (3) in the form of a solid support to which are bonded competitive ligands and/or recognition biological molecules at distinct and known recovery locations (4 and 5), which are arranged according to a two-dimensional matrix arrangement defined according to a system of coordinates, so as to identify by the localisation of said recovery locations (4 and 5) on said support, said analytes present in said sample (E).

公开(公告)号：US20230392184A1

公开(公告)日：2023-12-07

申请号：US18446048

申请日：2023-08-08

Applicant: PHAST CORP.

Inventor： Kwangmin SON ,  Roman STOCKER

IPC: C12Q1/04 ,  G02B21/00 ,  G02B21/36 ,  C40B30/06 ,  C40B40/02 ,  G01N33/94 ,  G16C10/00

CPC classification number: C12Q1/04 ,  G02B21/0084 ,  G02B21/365 ,  C40B30/06 ,  C40B40/02 ,  G01N33/9446 ,  G16C10/00

Abstract: Among other things, motility of at least one individual microorganism or a change in motility of at least one individual microorganism or both is or are characterized. The characterized motility or change in motility is used to detect the presence or count of the at least one individual microorganism, or determine the identity of a species or strain of the at least one individual microorganism, or determine a susceptibility of the at least one individual microorganism to one or more antibiotics or other antimicrobials.

公开(公告)号：US20230358741A1

公开(公告)日：2023-11-09

申请号：US18217635

申请日：2023-07-03

Applicant: HENAN POLYTECHNIC UNIVERSITY

Inventor： Jun XU ,  Lei JIA ,  Rui LI ,  Yongxin LI

IPC: G01N33/569 ,  G01N21/64 ,  G01N33/533 ,  G01N33/94 ,  C09K11/65 ,  C09K11/02

CPC classification number: G01N33/56911 ,  G01N21/6428 ,  G01N33/533 ,  G01N33/9446 ,  C09K11/65 ,  C09K11/02 ,  G01N2333/32 ,  B82Y20/00

Abstract: Provided is a dual-channel fluorescence sensor based on in-situ synthesis of carbon dots on halloysite nanotubes (HNT) and loaded with a lanthanide metal-organic framework, which can implement rapid and simultaneous visual detection of DPA and TC. By using methods for preparing and using a dual-channel visual multicolor fluorescent probe above, the sensor has high stability and sensitivity, and is conducive to quick, accurate and intuitive detection of a biomarker.

公开(公告)号：US20180011088A1

公开(公告)日：2018-01-11

申请号：US15546751

申请日：2016-01-27

Applicant: Neogen Corporation

Inventor： Andrew John Wheeler ,  Andrew John Denhartigh ,  Frank Eric Klein ,  David Phillip Ankrapp ,  Danielle Alexander

IPC: G01N33/543 ,  G01N33/545 ,  G01N33/553 ,  B01D15/38 ,  G01N33/94 ,  G01N33/558

CPC classification number: G01N33/54306 ,  B01D15/3809 ,  G01N33/54346 ,  G01N33/54386 ,  G01N33/545 ,  G01N33/553 ,  G01N33/558 ,  G01N33/9446

Abstract: The present disclosure provides a device and method for measuring an amount of an analyte in a sample, comprising a lateral flow matrix which defines a flow path and which comprises, in series: a sample receiving zone; a labeling zone comprising an unlabeled receptor and a labeled receptor, the unlabeled receptor located downstream of the labeled receptor and separated by a distance; and two serially oriented capture zones capable of providing quantitation of the amount of the analyte in the sample.

公开(公告)号：US20170176475A1

公开(公告)日：2017-06-22

申请号：US15302054

申请日：2015-04-09

Applicant: DREXEL UNIVERSITY

Inventor： Christopher VINNARD ,  Joseph M. SALVINO ,  Hans P. SCHLECHT

IPC: G01N33/94

CPC classification number: G01N33/9446 ,  A61K31/395 ,  A61K31/435 ,  A61K31/437 ,  A61K31/496 ,  G01N2800/52

Abstract: The present invention includes methods of identifying one or more rifamycins, or analogues or metabolites thereof, that are present in a biological sample from a subject that is being administered a rifamycin-containing medication. The present invention further includes methods of adjusting and/or optimizing the dosage regime for a rifamycin-containing medication.

公开(公告)号：US20170102382A1

公开(公告)日：2017-04-13

申请号：US15126796

申请日：2015-06-10

Applicant: Becton, Dickinson and Company

Inventor： James Raymond Petisce

IPC: G01N33/543

CPC classification number: G01N33/54386 ,  G01N33/9446

Abstract: Methods of evaluating a sample, e.g., a saliva sample, for the presence of an analyte, e.g., glucose, are provided. Aspects of the methods include: placing a sample onto a sample receiving location of a test strip device, where the test strip device includes analyte detection reagents; and then obtaining a signal from the test strip assay device to evaluate the sample for the presence of the analyte; where the methods include contacting the sample with an antibacterial agent at some point during the assay. Also provided are test strips and kits configured for use in the methods.

公开(公告)号：US09562898B1

公开(公告)日：2017-02-07

申请号：US14461742

申请日：2014-08-18

Applicant: Charm Sciences, Inc.

Inventor： Steven J. Saul ,  Robert J. Markovsky ,  David W. Douglas

IPC: G01N33/558 ,  G01N33/544

CPC classification number: G01N33/544 ,  G01N33/543 ,  G01N33/558 ,  G01N33/94 ,  G01N33/9446 ,  G01N2333/37

Abstract: Embodiments described herein include methods and assays for detecting beta-lactam analytes and mycotoxin analytes in a sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.

Abstract translation: 本文描述的实施方案包括用于检测样品中β-内酰胺分析物和霉菌毒素分析物的方法和测定。 一些实施方案包括利用多个分析物结合物检测样品中的多种分析物和含有多个控制区捕获剂的对照区。 在一些实施例中，多个控制区捕获剂在一个控制区内捕获多个粘合剂。 通过将控制区信号与测试区信号进行比较来确定测试结果。

公开(公告)号：US09441263B2

公开(公告)日：2016-09-13

申请号：US14111947

申请日：2012-04-14

Applicant: Scott C. Blanchard ,  Michael Brian Feldman ,  Leyi Wang ,  James H. Doudna Cate ,  Arto Pulk ,  Roger B. Altman ,  Michael R. Wasserman

Inventor： Scott C. Blanchard ,  Michael Brian Feldman ,  Leyi Wang ,  James H. Doudna Cate ,  Arto Pulk ,  Roger B. Altman ,  Michael R. Wasserman

IPC: C12Q1/68 ,  G01N33/542 ,  G01N33/94

CPC classification number: C12Q1/68 ,  G01N33/542 ,  G01N33/9446 ,  G01N2333/245

Abstract: The present invention relates to methods to identify molecules that binds in the neomycin binding pocket of a bacterial ribosome using structures of an intact bacterial ribosome that reveal how the ribosome binds tRNA in two functionally distinct states, determined by x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor (RRF) and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit (P/E) site. Additionally, the invention relates to various assays, including single-molecule assay for ribosome recycling, and methods to identify compounds that interfere with ribosomal function by detecting newly identified intermediate FRET states using known and novel FRET pairs on the ribosome. The invention also provides vectors and compositions with an N-terminally tagged S13 protein.

Abstract translation: 本发明涉及使用完整的细菌核糖体的结构鉴定在细菌核糖体的新霉素结合口袋中结合的分子的方法，其揭示了核糖体如何通过x射线晶体学确定的两个功能不同的状态结合tRNA。 一个国家在肽基-tRNA结合位点定位tRNA。 第二个完全旋转状态通过核糖体循环因子（RRF）稳定，并在杂交肽基/出口（P / E）位点中以高度弯曲的构象结合tRNA。 此外，本发明涉及各种测定法，包括用于核糖体回收的单分子测定法，以及通过使用核糖体上已知和新的FRET对检测新鉴定的中间FRET状态来鉴定干扰核糖体功能的化合物的方法。 本发明还提供具有N-末端标记的S13蛋白的载体和组合物。

公开(公告)号：US20160131669A1

公开(公告)日：2016-05-12

申请号：US14893756

申请日：2014-05-30

Applicant: NANJING XIANGZHONG BIOTECHNOLOGY CO., LTD

Inventor： Zhonghui Li ,  Zhoumin Li ,  Siyun Sun

IPC: G01N33/94

CPC classification number: G01N33/9446 ,  G01N33/6845 ,  G01N33/94 ,  G01N2415/00

Abstract: The present invention provides a biochip for simultaneously detecting a variety of antibiotics, illegal additives and biotoxins in a visualized manner, wherein the biochip comprises a chip carrier fixed with a group of detection target antigens, the detection targets are the antibiotics, the illegal additives and the biotoxins, and the biochip is prepared by the following method: enabling the bovine serum albumin and the detection target antigens to perform sample application operation on the chip carrier through a biochip preparation system by taking bovine serum albumin as blank control, and then fixing in a water bath at 20-37° C. for 0.5-4 h for preparation. The invention further provides a method for simultaneously detecting a variety of antibiotics, illegal additives and biotoxins by using the biochip. The biochip has the advantages of simple structure, simple preparation process, low cost, multiple targets, high accuracy, high sensitivity, high precision, short detection time, simpleness and easiness in operation and no need of expensive detection instruments and is applicable to on-site large-scale primary screening of samples.

Abstract translation: 本发明提供了一种用于以可视化方式同时检测各种抗生素，非法添加剂和生物毒素的生物芯片，其中生物芯片包括固定有一组检测靶抗原的芯片载体，检测目标是抗生素，非法添加剂和 生物毒素和生物芯片通过以下方法制备：使牛血清白蛋白和检测靶抗原通过生物芯片制备系统通过将牛血清白蛋白作为空白对照，在芯片载体上进行样品施用操作，然后固定在 20-37℃的水浴0.5-4小时进行制备。 本发明还提供了一种通过使用生物芯片同时检测各种抗生素，非法添加剂和生物毒素的方法。 该生物芯片结构简单，制备工艺简单，成本低，目标多，精度高，灵敏度高，精度高，检测时间短，操作简单，操作方便，无需昂贵的检测仪器， 现场大规模初步筛选样品。

公开(公告)号：US09234889B1

公开(公告)日：2016-01-12

申请号：US12640764

申请日：2009-12-17

Applicant: Steven J. Saul ,  David W. Douglas

Inventor： Steven J. Saul ,  David W. Douglas

IPC: G01N33/537 ,  G01N33/543

CPC classification number: G01N33/537 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/558 ,  G01N33/9446 ,  G01N2333/38 ,  G01N2333/385

Abstract: A method and test strip for detecting one or more analytes in a test solution. The method and test strip include a detectable component, such as a gold particle, that is linked to a binder, such as an antibody, through a linkage. The linkage can include an antibody binding protein and an antispecies antibody.

Abstract translation: 用于检测测试溶液中一种或多种分析物的方法和测试条。 方法和测试条包括可通过连接键连接到诸如抗体的粘合剂的可检测组分，例如金颗粒。 连接可以包括抗体结合蛋白和抗病毒抗体。