公开(公告)号：US20140161686A1

公开(公告)日：2014-06-12

申请号：US14102367

申请日：2013-12-10

Applicant: Advanced Liquid Logic, Inc.

Inventor： Donovan E. BORT ,  Vijay SRINIVASAN ,  Patrick STERLINA ,  Uichong B. YI ,  Justin CAPPELLETTI

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/045 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0683

Abstract: Microfluidic system including a droplet actuator having an interior cavity and a series of electrodes arranged along the interior cavity for forming a droplet-operation path therethrough. The droplet actuator has a module-engaging side including an opening that is in flow communication with the interior cavity. The microfluidic system also includes a reservoir module configured to be coupled to the droplet actuator. The reservoir module includes a plurality of liquid compartments having respective outlets and at least one seal positioned along the outlets to retain liquid within the liquid compartments. The reservoir module is movable along the module-engaging side of the droplet actuator to position the outlets relative to the opening. The microfluidic system also includes a piercer having a tip configured to penetrate the seal thereby permitting the liquid within the corresponding liquid compartment to flow into the opening.

Abstract translation: 微流体系统包括具有内腔的液滴致动器和沿内腔布置的一系列电极，用于形成通过其的液滴操作路径。 液滴致动器具有包括与内腔流动连通的开口的模块接合侧。 微流体系统还包括被配置为耦合到液滴致动器的贮存器模块。 储存器模块包括多个具有相应出口的液体隔室和沿出口定位的至少一个密封件，以将液体保持在液体隔间内。 储存器模块可沿液滴致动器的模块接合侧移动，以使出口相对于开口定位。 微流体系统还包括具有尖端的穿孔器，该尖端被配置为穿透密封，从而允许相应的液体隔室内的液体流入开口。

公开(公告)号：US20170266653A1

公开(公告)日：2017-09-21

申请号：US15614944

申请日：2017-06-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. POLLACK ,  Ramakrishna SISTA ,  Zhishan HUA ,  Vamsee K. PAMULA ,  Arjun SUDARSAN ,  Vijay SRINIVASAN ,  Prasanna THWAR ,  Alexander SHENDEROV ,  Dwayne ALLEN

IPC: B01L3/02 ,  B03C1/28 ,  B01F13/08 ,  B01L3/00 ,  B01F13/00 ,  B03C1/01

CPC classification number: B01F13/0023 ,  B01F13/002 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0818 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0631 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/0478 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/284 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  Y10T436/25

Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.