Abstract:
Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.
Abstract:
This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., d-amino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor a 2-oxo acid+NH3+reduced acceptor.
Abstract translation:本发明一般涉及酶,编码酶的多核苷酸,这种多核苷酸和多肽的用途,更具体地涉及具有转移酶活性的酶,例如转氨酶活性,例如d-氨基酸转移酶活性和/或氧化还原酶活性,例如 ,脱氢酶活性,例如,d-氨基酸脱氢酶活性和/或催化化学基团的转移,催化转氨酶,催化反应:D-丙氨酸+ 2-氧戊二酸酯<=>丙酮酸盐+ D-谷氨酸盐和/ 或催化氧化还原反应,催化氢原子的去除和/或催化反应:D-氨基酸+ H 2 O +受体<=> 2-氧代酸+ NH 3 +还原受体。
Abstract:
Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins, other phenolic, and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.