公开(公告)号：US11046961B2

公开(公告)日：2021-06-29

申请号：US16700322

申请日：2019-12-02

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US20140370569A1

公开(公告)日：2014-12-18

申请号：US14374556

申请日：2013-01-29

Applicant: BASF SE

Inventor： Tobias Kueppers ,  Victoria Steffen ,  Renee Charlott Eichstadt ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts ,  Hendrik Hellmuth ,  Thomas Weber ,  Timothy O'Connell

IPC: C12N9/26 ,  C12N9/50

CPC classification number: C12N9/2411 ,  C12N9/00 ,  C12N9/2414 ,  C12N9/50 ,  C12N9/54 ,  C12N15/75 ,  C12P21/02

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Abstract translation: 在微生物发酵中，目的是提高蛋白质的产率。 这是通过一种方法实现的，其中将表达构建体引入到包含启动子和编码该蛋白质的核酸的物种短小芽孢杆菌的微生物中，并且所述蛋白质在所述表达构建体中表达。

公开(公告)号：US20200095564A1

公开(公告)日：2020-03-26

申请号：US16700322

申请日：2019-12-02

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maure ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/75 ,  C12N15/70 ,  C12N15/62

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US20180073005A1

公开(公告)日：2018-03-15

申请号：US15712652

申请日：2017-09-22

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

CPC classification number: C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US09725704B2

公开(公告)日：2017-08-08

申请号：US14374556

申请日：2013-01-29

Applicant: BASF SE

Inventor： Tobias Kueppers ,  Victoria Steffen ,  Renee Charlott Eichstadt ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts ,  Hendrik Hellmuth ,  Thomas Weber ,  Timothy O'Connell

IPC: C12N9/26 ,  C12N9/50 ,  C12N1/20 ,  C12N15/74 ,  C12P21/06 ,  C12P19/34 ,  C12N15/75 ,  C12P21/02 ,  C12N9/00 ,  C12N9/54

CPC classification number: C12N9/2411 ,  C12N9/00 ,  C12N9/2414 ,  C12N9/50 ,  C12N9/54 ,  C12N15/75 ,  C12P21/02

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.