公开(公告)号：US11299522B2

公开(公告)日：2022-04-12

申请号：US15779595

申请日：2016-12-02

Applicant: BASF SE

Inventor： Stefan Haefner ,  Andreas Thywissen ,  Holger Hartmann ,  Nico Boehmer

IPC: C12P21/00 ,  C12N9/14 ,  C12N1/15 ,  C07K14/37 ,  C12N9/16 ,  C12N9/24 ,  C12N15/80

Abstract: The present invention relates to a method of producing a recombinant polypeptide a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 2 (CLR2) and to express said recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR2 and the use of this filamentous fungus in the production of a recombinant polypeptide.

公开(公告)号：US11279955B2

公开(公告)日：2022-03-22

申请号：US16614772

申请日：2018-05-18

Applicant: BASF SE

Inventor： Hartwig Schroeder ,  Stefan Haefner ,  Oskar Zelder ,  Christoph Wittmann ,  Anna Christine Schroer ,  Birgit Hoff ,  Nico Boehmer

IPC: C12N1/20 ,  C12P7/18 ,  C12P7/46

Abstract: Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

公开(公告)号：US20200308235A1

公开(公告)日：2020-10-01

申请号：US15779595

申请日：2016-12-02

Applicant: BASF SE

Inventor： Stefan Haefner ,  Andreas Thywissen ,  Holger Hartmann ,  Nico Boehmer

IPC: C07K14/37 ,  C12N9/16 ,  C12N9/24 ,  C12N15/80

Abstract: The present invention relates to a method of producing a recombinant polypeptide a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 2 (CLR2) and to express said recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR2 and the use of this filamentous fungus in the production of a recombinant polypeptide.

公开(公告)号：US20180354999A1

公开(公告)日：2018-12-13

申请号：US15779646

申请日：2016-12-02

Applicant: BASF SE

Inventor： Stefan Haefner ,  Andreas Thywissen ,  Holger Hartmann ,  Nico Boehmer

IPC: C07K14/37 ,  C12Q1/34

CPC classification number: C07K14/37 ,  C12N1/14 ,  C12N9/2437 ,  C12N15/01 ,  C12N15/80 ,  C12P19/14 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/34

Abstract: The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.

公开(公告)号：US20200181653A1

公开(公告)日：2020-06-11

申请号：US16614772

申请日：2018-05-18

Applicant: BASF SE

Inventor： Hartwig Schroeder ,  Stefan Haefner ,  Oskar Zelder ,  Christoph Wittmann ,  Anna Christine Schroer ,  Birgit Hoff ,  Nico Boehmer

IPC: C12P7/18 ,  C12N1/20

Abstract: Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

公开(公告)号：US10597429B2

公开(公告)日：2020-03-24

申请号：US15779646

申请日：2016-12-02

Applicant: BASF SE

Inventor： Stefan Haefner ,  Andreas Thywissen ,  Holger Hartmann ,  Nico Boehmer

IPC: C12P21/00 ,  C12P21/02 ,  C12Q1/34 ,  C12N1/14 ,  C12N15/01 ,  C12N15/80 ,  C12P19/14 ,  C07K14/37 ,  C12N9/42

Abstract: The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.