公开(公告)号：US20240101971A1

公开(公告)日：2024-03-28

申请号：US18273053

申请日：2022-01-21

Applicant: Biogen MA Inc.

Inventor： Terrence Michael Dobrowsky ,  Jenny Wang-Yuanzhen Shupe

IPC: C12N7/00 ,  C12M1/00 ,  C12N15/85

CPC classification number: C12N7/00 ,  C12M29/10 ,  C12N15/85 ,  C12N2750/14122 ,  C12N2750/14123 ,  C12N2750/14152 ,  C12N2800/107

Abstract: The present disclosure pertains to methods and compositions for the production of recombinant viral vectors, e.g., recombinant adeno-associated viruses (rAAVs), using at least one microtubule destabilizing agent described herein.

公开(公告)号：US20170029859A1

公开(公告)日：2017-02-02

申请号：US15291257

申请日：2016-10-12

Applicant: BIOGEN MA INC.

Inventor： Alan Gilbert ,  Kyle McElearney ,  Terrence Michael Dobrowsky ,  Rashmi Rohit Kshirsagar

IPC: C12P21/00 ,  C07K14/755 ,  C12N5/00

CPC classification number: C07K14/755 ,  C12N5/0018 ,  C12N2500/24 ,  C12N2500/34 ,  C12N2510/02 ,  C12N2511/00 ,  C12P21/02

Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

Abstract translation: 本发明涉及包含硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物的细胞培养基及其使用方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物补充细胞培养物。