公开(公告)号：US11857968B2

公开(公告)日：2024-01-02

申请号：US17357860

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  C12Q1/6869 ,  B01L3/502776 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2400/0487 ,  Y10T436/2575

Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.

公开(公告)号：US11926871B2

公开(公告)日：2024-03-12

申请号：US17215683

申请日：2021-03-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2565/514

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.

公开(公告)号：US11045805B2

公开(公告)日：2021-06-29

申请号：US16178300

申请日：2018-11-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

Abstract: Microfluidic systems and methods for arranging a set of objects. In an exemplary method, the set of objects may be transported in carrier fluid along a microfluidic channel structure having a reformatting zone including an object-accessible region and at least one object-excluding region. A portion of the carrier fluid may be moved from the object-accessible region to the at least one object-excluding region in an upstream section of the reformatting zone, to reduce a spacing of objects of the set. The portion of the carrier fluid may be directed into the object-accessible region from the at least one object-excluding region in a downstream section of the reformatting zone, to increase a spacing of objects of the set. The steps of moving and directing in combination may increase the spacing between objects disproportionately for a subset of the objects that are closest to one another.

公开(公告)号：US12209277B2

公开(公告)日：2025-01-28

申请号：US16260475

申请日：2019-01-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sean Cater ,  Lucas Frenz ,  Andrew Kohlway ,  Jennifer Chew ,  Rob Ray ,  Ronald Lebofsky

IPC: C12Q1/6853 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods are provided of assessing whether multiple differently-barcoded primers occur within the same partition.

公开(公告)号：US10988808B2

公开(公告)日：2021-04-27

申请号：US16802264

申请日：2020-02-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.

公开(公告)号：US20210316309A1

公开(公告)日：2021-10-14

申请号：US17357860

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.

公开(公告)号：US20170198345A1

公开(公告)日：2017-07-13

申请号：US15404980

申请日：2017-01-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2565/514

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.