公开(公告)号：US20210207208A1

公开(公告)日：2021-07-08

申请号：US17148440

申请日：2021-01-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas HEREDIA ,  Dianna MAAR

IPC: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US20190211388A1

公开(公告)日：2019-07-11

申请号：US16361704

申请日：2019-03-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas HEREDIA ,  Dianna MAAR

IPC: C12Q1/6865 ,  C12Q1/6858 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q1/6848 ,  C12Q1/6853

CPC classification number: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2565/1015

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US20240124927A1

公开(公告)日：2024-04-18

申请号：US18403525

申请日：2024-01-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas HEREDIA ,  Dianna MAAR

IPC: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858

CPC classification number: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US20230390726A1

公开(公告)日：2023-12-07

申请号：US18233235

申请日：2023-08-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Nicholas HEREDIA

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00722

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US20200324264A1

公开(公告)日：2020-10-15

申请号：US16911961

申请日：2020-06-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Nicholas HEREDIA

IPC: B01J19/00 ,  C12Q1/6806

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.