公开(公告)号：US08877478B2

公开(公告)日：2014-11-04

申请号：US12442207

申请日：2007-09-21

申请人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

发明人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

IPC分类号： C12N9/16 ,  C12N11/00 ,  C12P21/06 ,  C12P7/02 ,  C11C1/00 ,  C12P1/00 ,  A23L1/10 ,  A23C9/12 ,  A61K38/46 ,  C12P7/10

CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

摘要翻译： 本发明涉及植酸酶，编码它们的多核苷酸，本发明的多核苷酸和多肽的用途，以及这些多核苷酸和多肽的产生和分离。 特别地，本发明提供了在高温条件下具有植酸酶活性的多肽，以及暴露于高温后保留活性的植酸酶。 除了较高的温度之外，本发明的植酸酶还可以在低温下耐热和/或耐热。 本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以配制成食物或饲料或补充剂，用于例如帮助消化植酸盐。 本发明的食品或饲料可以是丸粒，液体，粉末等的形式。 在一个方面，本发明的植酸酶在造粒期间对于热变性是稳定的; 并且这降低了植酸酶产品的成本，同时保持了体内功效并检测了饲料中的活性。